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논문 상세정보

Abstract

A simple and convenient method of immobilizing dextransucrase via an affinity interaction is described, along with the use of this system to synthesize leucrose. Dextransucrase was produced in sucrose-free medium by fermenting a constitutive mutant of Leuconostoc mesenteroides NRRL B-512F and was separated using an ultrafiltration membrane. The purified enzyme was free of dextran polymer, which previously was always found with the sucrose-induced enzyme. Therefore, it was possible to immobilize the enzyme on dextran-based resins using an affinity interaction. Sephadex G-200 was the best resin for immobilizing the dextransucrase and gave a fast flow rate through the packed column. The immobilized dextransucrase retained more than 80% of its specific activity after immobilization ($K_m\;=\;18.1\;mM$ and $k_{cat}\;=\;450\;sec^{-1}$ vs. 13.1 mM and $640\;sec^{-1}$, respectively, for the free enzyme). The immobilized dextransucrase showed improved stability over a pH range of 4.0 to 6.5 and at moderately high temperatures over $40^{\circ}C$. When immobilized dextransucrase was used to synthesize leucrose via the transfer reaction with sucrose and fructose, about 74% of the sucrose was converted into leucrose after one day, and the half-life of the enzyme activity was 15 days. Regeneration of the resin by supplementation with dextransucrase enabled the recovery of the initial activity of the system, but both the reaction and the flow rate were lower, probably owing to the accumulation of dextran inside the resin.

참고문헌 (34)

  1. Dextrans;Industrial Gums , De Belder, A.N.;Whistler, R.L.(ed.);BeMiller, J.N.(ed.) , / v.,pp.399-425, 1993
  2. The mechanism of acceptor reactions of Leuconostoc mesenteroides B-512F dextransucrase , Robyt, J.F.;Walseth, T.E. , Carbohydr. Res. / v.61,pp.433-445, 1978
  3. Relative, quantitative effects of acceptors in the reaction of Leuconostoc mesenteroides B-512F dextransucrase , Robyt, J.F.;Eklund, S.H. , Carbohydr. Res. / v.121,pp.279-286, 1983
  4. Leucrose, a ketodisaccharide of industrial design;Carbohydrates as Organic Raw Materials , Schwengers, D.;Lichtenthaler, F.W.(ed.) , / v.,pp.183-195, 1991
  5. Immobilization and properties of Leuconostoc mesenteroides dextransucrase , Kaboli, H.;Reilly, P.J. , Biotech. Bioeng. / v.22,pp.1055-1069, 1980
  6. On the production of dextran by free and immobilized dextransucrase , Monsan, P.;Lopez, A. , Biotech. Bioeng. / v.23,pp.2027-2037, 1981
  7. Investigation of production of dextran and dextransucrase by Leuconostoc mesenteroides immobilized within porous stainless steel , El-Sayed, A.M.M.;Abdul-Wahid, K.;Coughlin, R.W. , Biotech. Bioeng. / v.40,pp.617-624, 1992
  8. Unconventional immobilization of dextransucrase with alginate , Reischwitz, A.;Reh, K.D.;Buchholz, K. , Enzyme Microb. Technol. / v.17,pp.457-461, 1995
  9. Productivity of immobilized dextransucrase for leucrose formation , Reh, K.D.;Noll-Borchers, M.;Buchholz, K. , Enzyme Microb. Technol. / v.19,pp.518-524, 1996
  10. Hemoglobin encapsulation in chitosan/calcium alginate beads , Huguet, M.L.;Groboillot, A.;Neufeld, R.J.;Poncelet, D.;Dellacherie, E. , J. Appl. Polymer Sci. / v.51,pp.1427-1432, 1994
  11. Calcium-alginate beads coated with polycationic polymers: comparison of chitosan and DEAE-dextran , Huguet, M.L.;Neufeld, R.I.;Dellacherie, E. , Process Biochem. / v.31,pp.347-353, 1996
  12. Cloning and sequencing of a gene coding for a novel dextransucrase from Leuconostoc mesenteroides NRRL B-1299 synthesizing only ${\alpha}$(1-6) and ${\alpha}$(1-3) linkages , Monchois, B.;Willemot, R.M.;Remaud-Simeon, M.;Croux, C.;Monsan, P. , Gene / v.182,pp.23-32, 1996
  13. Aggregated form of dextransucrases from Leuconostoc mesenteroides NRRL B-512F and its constitutive mutant , Funane, K.;Yamada, M.;Shiraiwa, M.;Takahara, H.;Yamamoto, N.;Ichishima, E.;Kobayashi, M. , Biosci. Biotech. Biochem. / v.53,pp.776-780, 1995
  14. Large-scale preparation of highly purified dextransucrase from a high-producing constitutive mutant of Leuconostoc mesenteroides B-512FMC , Kitaoka, M.;Robyt, J.F. , Enzyme Microb. Technol. / v.23,pp.386-391, 1998
  15. Production and selection of mutants of Leuconostoc mesenteroides constitutive for glucansucrases , Kim, D.;Robyt, J.F. , Enzyme Microb. Technol. / v.16,pp.659-664, 1994
  16. Mechanism of dextran activation of dextransucrase , Robyt, J.F.;Kim, D.;Yu, L. , Carbohydr. Res. / v.266,pp.293-299, 1995
  17. Properties of Leuconostoc mesenteroides B-512FMC constitutive dextransucrase , Kim, D.;Robyt, J.F. , Enzyme Microb. Technol. / v.16,pp.1010-1015, 1994
  18. Milligram to gram scale purification and characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F , Miller, A.W.;Eklund, S.H.;Robyt, J.F. , Carbohydr. Res. / v.147,pp.119-133, 1986
  19. Characterization of the multiple forms and main component of dextransucrase from Leuconostoc mesenteroides NRRL B-512F , Kobayashi, M.;Matsuda, K. , Biochem. Biophys. Acta / v.614,pp.46-62, 1980
  20. Immobilization of thermostable maltogenic amylase from Bacillus stearothermophilus for continuous production of branched oligosaccharides , Kang, G.J.;Kim, M.J.;Kim, J.W.;Park, K.H. , J. Agric. Food Chem. / v.45,pp.4168-4172, 1997
  21. Covalent immobilization of ${\alpha}$-amylase onto PHEMA microspheres: preparation and application to fixed bed reactor , Agric, M.Y.;Hasirci, V.;Alaeddinoglu, N.G. , Biomaterials / v.13,pp.704-709, 1995
  22. Entrapment of urease in glycol containing polymeric matrices and estimation of effective diffusion coefficients of urea , Dermircioglu, H.;Beyenal, H.;Tanyolac, A.;Hasirci, N. , Polymer / v.36,pp.4091-4096, 1995
  23. Mechanisms in the glucansucrase synthesis of polysaccharides and oligosaccharides from sucrose , Robyt, J.F. , Adv. Carbo. Chem. Biochem. / v.51,pp.133-168, 1995
  24. The preparation, properties and structure of the disaccharide leucrose , Stodola, F.H.;Sharpe, E.S.;Koepsell, H.J. , J. Am. Chem. Soc. / v.78,pp.2514-2518, 1956
  25. Dextran synthesis using immobilized Leuconostoc mesenteroides dextransucrase , Monsan, P.;Paul, F.;Auriol, D.;Lopez, A. , Methods Enzymol. / v.136,pp.239-254, 1987
  26. In vitro synthesis of oligosaccharides by acceptor reaction of dextransucrase from Leuconostoc mesenteroides , Pereira, A.M.;Costa, F.A.;Rodrigues, M.I.;Maugeri, F. , Biotechnol. Lett. / v.20,pp.397-401, 1998
  27. Bioaffinity based immobilization of enzymes , Saleemuddin, M. , Adv. Biochem. Eng. Biotechnol. / v.64,pp.203-226, 1999
  28. Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli , Schatz, P.J. , Biotechnology / v.11,pp.1138-1143, 1993
  29. Calmodulin as a versatile tag for antibody fragments , Neri, D.;De Lalla, C.;Petrul, H.;Neri, P.;Winter, G. , Biotechnology / v.13,pp.373-377, 1995
  30. Solid-phase refolding of cyclodextrin glycosyltransferase adsorbed on cationic-exchange resin , Kweon, D.H.;Lee, D.H.;Han, N.S.;Seo, J.H. , Biotech. Prog. / v.20,pp.277-283, 2004
  31. Improved adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding domain from Aspergillus glucoamylase , Chen, L.J.;Ford, C.;Kusnadi, A.;Nikolov, Z.L. , Biotechnol. Prog. / v.7,pp.225-229, 1991
  32. Separation of MBP fusion proteins through affinity membranes , Cattoli, F.;Sarti, G.C. , Biotechnol. Prog. / v.18,pp.94-100, 2002
  33. Cellulose-binding domains: biotechnological applications , Levy, I.;Shoseyov, O. , Biotechnol. Adv. / v.20,pp.191-213, 2002
  34. Conserved repeat motifs and glucan binding by glucansucrases of oral streptococci and Leuconostoc mesenteroides , Shah, D.S.;Joucla, G.;Remaud-Simeon, M.;Russell, R.R. , J. Bacteriol. / v.186,pp.8301-8308, 2004

이 논문을 인용한 문헌 (2)

  1. 2005. "" Food science and biotechnology, 14(6): 818~822 
  2. 2009. "" Food science and biotechnology, 18(5): 1083~1090 

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