Abstract

This study evaluated the binding abilities of rabbit anti-ovalbumin (OVA) immunoglobulin G (IgG) and egg-allergic patient IgE on gamma-irradiated OVA during proteolysis using pepsin and trypsin. The concentrations of both the intact and the irradiated OVAs decreased during proteolysis when detected with IgG However, when detected by patient IgE the concentration of the intact OVA decreased up to 30 min after the trypsin treatment and increased thereafter. Irradiated OVA detected by patient IgE showed a lower initial concentration (0.16%) than that of the intact OVA, and this reduced concentration was maintained stably. The results indicate that irradiation, rather than enzymatic treatment, could reduce the binding of the irradiated and enzyme-treated OVA. Therefore, gamma irradiation has potential as an effective method to reduce OVA-induced allergy and may enhance the safety of egg-allergic individuals.

주제어

#ovalbumin   #gamma irradiation   #proteolysis   #IgE-binding ability  

저자의 다른 논문

참고문헌 (17)

1. Food allergy. Food allergy Part 1: immunopathogenesis and clinical disorders , Sampson, H.A. , J. Allergy Clin. Immunol. / v.103,pp.717-728, 1999
   
2. The chemistry and biology of food allergens , Hefle, S.L. , Food Technol. / v.,pp.86-89, 1996
   
3. Stability of food allergens to digestion in vitro , Astwood, J.D.;Leach, J.N.;Ruchs, R.L. , Nat. Biotechnol. / v.14,pp.1269-1273, 1996
   
4. Digestibility of food allergens and nonallergic proteins in simulated gastric fluid and simulated intestinal fluid-a comparative study , Fu, T.J.;Abbott, U.R.;Hatzos, C. , J. Agric. Food Chem. / v.50,pp.7154-7160, 2002
   
5. The nature and mechanism of food allergies and related diseases , Metcalfe, D.D. , Food Technol. / v.,pp.136-139, 1992
   
6. The role of epithelial cells in immune regulation in the gut , Shao, L.;Serrano, D.;Mayer, L. , Semi. Immunol. / v.13,pp.163-175, 2001
   
7. Antigen absorption;Food allergy: Adverse reactions to foods and food additives , Murphy, S.;Walker, W.A. , / v.,pp.93-106, 1997
   
8. 9. Food allergy , Sampson, H.A. , J. Allergy Clin. Immunol. / v.111,pp.S540-S547, 2003
   
9. Mechanism and control of food allergy , Hayakawa, K.;Kinko, Y.;Linko, P. , Lebensm-Wiss. U-. Technol. / v.32,pp.1-11, 1999
   
10. Change in structural and antigenic properties of proteins by radiation , Kume, T.;Matsuda, T. , Radiat. Phys. Chem. / v.46,pp.225-231, 1995
    
11. Effects of heat treatment on the antigenicity and gamma-irradiated egg white albumin , Lee, J.W.;Yook, H.S.;Cho, K.H.;Kim, C.J.;Byun, M.W. , J. Korean Soc. Food Sci. Nutr. / v.30,pp.848-853, 2001
    원문보기 상세보기
12. The changes of allergenic and antigenic properties of egg white albumin (Gal d 1) by gamma irradiation , Lee, J.W.;Yook, H.S.;Cho, K.H.;Lee, S.Y.;Byun, M.W. , J. Korean Soc. Food Sci. Nutr. / v.30,pp.500-504, 2001
    원문보기 상세보기
13. Characterization of anti-irradiation-denatured ovalbumin monoclonal antibodies. Immunochemical and structural analysis of irradiation-denatured ovalbumin , Masuda, T.;Koseki, S.Y.;Yasumoto, K.;Kitabatake, N. , J. Agric. Food Chem. / v.48,pp.2670-2674, 2000
    
14. Reduced allergenicities of irradiated egg white ovalbumin determined by skin prick test and ELISA inhibition test , Jeon, G.R.;Lee, J.W.;Byun, M.W.;Lee, S.Y. , J. Asthma Allergy Clin. Immunol. / v.22,pp.711-719, 2002
    
15. Change of an egg allergen in a white layer cake containing gamma-irradiated egg white , Seo, J.H.;Lee, J.W.;Lee, Y.S.;Lee, S.Y.;Kim, M.R.;Yook, H.S.;Byun, M.W. , J. Food Prot. / v.67,pp.1463-1468, 2004
    
16. Immunochemical and structural analysis of pepsin-digested egg white ovomucoid , Kovacs-Nolan, J.;Zhang, J.W.;Shigeru, H.;Mine, Y. , J. Agric. Food Chem. / v.48,pp.6261-6266, 2000
    
17. SAT/STAT: User's Guide , SAS , / v.,pp., 1998
    

이 논문을 인용한 문헌 (2)

1. 2006. "" Food science and biotechnology, 15(6): 954~958  원문보기 상세보기
2. 2008. "" Food science and biotechnology, 17(5): 919~924  원문보기 상세보기