The aim of this study was to investigate the in vitro antioxidant profiles of genistein and other isoflavonoids. The reactivity of genistein towards stable radical and reactive oxygen species including ${\bullet}\;ABTS^+$, ${\bullet}{O_2}^-$, $H_2O_2$ and HOCl has be...
The aim of this study was to investigate the in vitro antioxidant profiles of genistein and other isoflavonoids. The reactivity of genistein towards stable radical and reactive oxygen species including ${\bullet}\;ABTS^+$, ${\bullet}{O_2}^-$, $H_2O_2$ and HOCl has been investigated, and the effects were compared with other isoflavonoids and antioxidants. All the tested isoflavonoids showed remarkable ${\bullet}\;ABTS^+$ scavenging activity and genistein was more potent than BHT and ascorbic acid. Genistein was more effective in scavenging hypochlorous acid than superoxide and hydrogen peroxide. At $10\;{\mu}M$ concentrations of genistein and genistin showed about 90% inhibitory effect on HOCl, while BHT and ascorbic acid showed lower than 50% inhibitory effect. Moreover, genistein could inhibit plasmid DNA cleavage, protein degradation and cell death from HOCl attack, while daidzein, BHT and ascorbic acid could not protect them effectively. These results suggest that genistein is a more potent radical scavenger than other isoflavonoids, and it can remarkably reduce cellular damage induced by HOCl.
The aim of this study was to investigate the in vitro antioxidant profiles of genistein and other isoflavonoids. The reactivity of genistein towards stable radical and reactive oxygen species including ${\bullet}\;ABTS^+$, ${\bullet}{O_2}^-$, $H_2O_2$ and HOCl has been investigated, and the effects were compared with other isoflavonoids and antioxidants. All the tested isoflavonoids showed remarkable ${\bullet}\;ABTS^+$ scavenging activity and genistein was more potent than BHT and ascorbic acid. Genistein was more effective in scavenging hypochlorous acid than superoxide and hydrogen peroxide. At $10\;{\mu}M$ concentrations of genistein and genistin showed about 90% inhibitory effect on HOCl, while BHT and ascorbic acid showed lower than 50% inhibitory effect. Moreover, genistein could inhibit plasmid DNA cleavage, protein degradation and cell death from HOCl attack, while daidzein, BHT and ascorbic acid could not protect them effectively. These results suggest that genistein is a more potent radical scavenger than other isoflavonoids, and it can remarkably reduce cellular damage induced by HOCl.
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제안 방법
The objectives of the present study were to determine the radical scavenging properties of genistein and isoflavonoids by in vitro assays, to evaluate the specific ROS scavenging activity of genistein on HOC1 in order to understand its role in the prevention of ROS-induced damage in human diseases and to elucidate the relation ships between the structure of isoflavonoids and its antioxidant activity.
대상 데이터
Genistin, genistein (4' , 5, 7-trihydroxyisoflavone), daidzein (4'7-dihydroxyisoflavone), biochanin A, butylated hydroxy toluene (BHT), L-ascorbic acid, nitroblue tetrazolium (NBT), p-nicotinamide adenine dinucleotide reduced form (NADH), phenazine methosulfate (PMS), guaiacol, horseradish peroxidase (HRP), 2, 2-azino-bis (3-ethyl-benzthiazoline-6-sulfdnic acid) (ABTS), and trolox were purchased from Sigma Chemical Company (St. Louis, MO, USA). Sodium hypochloride was from Aldrich Chemical Company (Milwaukee, WI, USA).
Louis, MO, USA). Sodium hypochloride was from Aldrich Chemical Company (Milwaukee, WI, USA). Watersoluble tetrazolium, which is the sodium salt of 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5 ・tetrazoliol-1, 3 -benzene disulfate (WST-1), was purchased from Roche (Germany).
데이터처리
All statistical tests were performed using SPSS for windows (version 13). Statistical analysis of group differences was examined by using the non parametric Mann-Whitney-test. P values of <0.
성능/효과
Genistein had more than 80% of relative inhibition at 10 jiM concentration (Table 2). All the tested isoflavonoids in this assay showed remarkable HOC1 scavenging activities, while BHT and ascorbic acid showed lower than 40% of relative inhibition. In terms of HOC1 scavenging, the number of oxidizing groups like - OH and methoxyl group are more important than the position of hydroxyl group in B ring.
In conclusion, o니r results demonstrated that genistein showed inhibitory effect on several major ROS as previously reported, but genistein was also evaluated as a potent HOCI scavenger and it could protect proteins, DNA and live cells from HOCI attack. This study implies the possibility of therapeutic effect of genistein on inflammatory diseases.
The number and the position of hydroxyl groups and oxidizing gro니ps for electron donation are the keys for the activity of antioxidants. These results showed that the number of oxidizing groups like -OH and methoxyl groups in their structure is the critical factor for HOCI scavenging activity, because -OH and methoxyl groups could be chlorinated by HOCI (30). The chlorination of three -OH groups in genistein is a possible mechanism of antioxidant activity in HOCl-induced protein degradation, DNA strand cleavage and cell death.
후속연구
Also, the intake of soy containing food might contribute positive effect in allergic patients. Further studies are necessary to elucidate anti-inflammatory activity of genistein at the cellular and molecular levels. Acknowledgments
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