Abstract

To develop functional food and anti-obesity drug through inhibition of dietary lipid absorption, inhibitory effects of herb extracts on pancreatic lipase were investigated. Due to high yield and simplicity of isolation, lipase inhibitor (ELI) was isolated from ethyl acetate extract of Eugenia aromaticum, which showed highest inhibitory activity, and characterized for development of novel functional material. Stability of ELI at high temperature and low pH was investigated. Results showed ELI is relatively stable under thermal and acidic conditions, reversible, and noncompetitive inhibitor of pancreatic lipase.

주제어

#Lipase inhibitor   #Eugenia aromaticum   #Obesity   #Pancreatic lipase  

저자의 다른 논문

참고문헌 (22)

1. Orlistat:its current status as an anti-obesity drug , Ballinger, A.;Peikin, S.I.R. , Eur. J. Pharm. / v.440,pp.109-117, 2002
   
2. The underlying basis for obesity:relationship to cancer , Bray, G.A. , J. Nutr. / v.132,pp.3451S-3455S, 2002
   
3. Dietary fat intake does affect obesity , Bray, G.A.;Popkin, B.M. , Am. J. Clin. Nutr. / v.68,pp.1157-1173, 1998
   
4. Dietary fat affects obesity rate , Bray, G.A.;Popkin, B.M. , Am. J. Clin. Nutr. / v.70,pp.572-573, 1999
   
5. Orlistat (Ro 18-0647), a lipase inhibitor,in the treatment of human obesity:a multiple dose study , Drent, M.L.;Larsson, I.;William-Olsson, T.;Quaade, F.;Czubayko, F.;Bergmann, K.;Strobel, W.;Sjostrom, L.;van der Veen, E.A. , Int. J. Obesity / v.19,pp.221-226, 1995
   
6. Structure and function of gastrointestinal lipases , Embleton, J.K.;Pouton, C.W. , Adv. Drug. Deliv. Rev. / v.25,pp.15-32, 1997
   
7. Prevalence and trends in obesity among US adults,1999-2000 , Flegal, K.M.;Carroll, M.D.;Ogden, C.L.;Johnson, C.L. , JAMA / v.288,pp.1723-1727, 2002
   
8. Covalent inhibition of digestive lipases an in vitro study , Garugouri, Y.;Ransac, S.;Verger, R. , Biochimica. et Biophysica. Acta. / v.1334,pp.6-37, 1997
   
9. Studies on the inhibition of pancreatic and microbial lipases by soybean proteins , Garugouri, Y.;Julien, R.;Pieroni, G.;Verger, R.;Sarda, L. , J. Lipid Res. / v.25,pp.1214-1221, 1984
   
10. Anti-obesity effects in rodents of dietary teasaponin, lipase inhibitor , Han, L.K.;Kimura, Y.;Kawashima, M.;Takaku, T.;Taniyama, T.;Hayashi, T.;Zheng, Y.N.;Okuda, H. , Int. J. Obes. Rel. Metab Dis. / v.25,pp.1459-1464, 2001
    
11. Flavin dimers with lipase inhibitory activity from Cassia nomame , Hatano, T.;Yamashita, A.;Hashimoto, T.;Ito, H.;Kubo, N.;Yoshiyama, M.;Shimura, S.;Itoh, Y.;Okuda, T.;Yoshida, T. , Phytochemistry / v.46,pp.893-900, 1997
    
12. Lipase inhibitor for the treatment of acne , Higaki, S. , J. Molecular Catalysis B : Enzymatic. / v.22,pp.377-384, 2003
    
13. Green tea extract $(AR25^{\circledR})$ inhibits lipolysis of triglycerides in gastric and duodenal medium in vitro , Juhel, C.;Armand, M.;Pafumi, Y.;Rosier, C.;Vandermander, J.;Lairon, D. , J. Nutr. Biochem. / v.11,pp.45-51, 2000
    
14. Control of canine gastric emptying of fat by lipolytic products , Meyer, J.H.;Elashoff, J.D.;Domeck, M.;Levy, A.;Jehn, D.;Hlinka, M.;Lake, R.;Graham, L.S.;Gu, Y.G. , Am. J. Physiol. / v.266,pp.1017-1035, 1994
    
15. Role of small intestine in caloric compensations to oil premeals in rats , Meyer, J.H.;Hlinka, M.;Khatibi, A.;Raybould, H.E.;Tso, P. , Am. J. Physiol. / v.275,pp.R1320-R1333, 1998
    
16. A new spectrophotometric method for the detection of lipase activity using 2,4-dinitrophenyl butyrate as a substrate , Mosmuller, E.W.J.;Van Heemst, J.D.H.;Van Delden, C.J.;Franssen, M.C.R.;Engbersen, J.F.J. , Biocatalysis / v.5,pp.279-287, 1992
    
17. Effect of Orlistat-Assisted Weight Loss in Decreasing Coronary Heart Disease Risk in Patients with Syndrome X , Reaven, G.;Segal, K.;Hauptman, J.;Boldrin, M.;Lucas, C. , Am. J. Cardiol. / v.87,pp.827-831, 2001
    
18. Inhibitor Effect on Lipase Activity of Extracts from Medicinal Herbs , Shimura, S.;Tsuzuki, W.;Kobayashi, S.;Suzuki, T. , Biosci. Biotech. Biochem. / v.56,pp.1478-1479, 1992
    
19. Degree of in vivo inhibition of human gastric and pancreatic lipases by Orlistat (Tetrahydrolipstatin,THL) in the stomach and small intestine , Sternby, B.;Hartmann, D.;Borgsrom, B.;Nilsson, A. , Clin. Nutr. / v.21,pp.395-402, 2002
    
20. Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame herba, on rats fed a high-fat diet , Yamamoto, M.;Shimura, S.;Itoh, Y.;Ohsaka, T.;Egawa, M.;Inoue, S. , Int .J. Obes. / v.24,pp.758-764, 2000
    
21. Obesity , Yanovski, S.Z.;Yanovski, J.A. , N. Engl. J. Med. / v.346,pp.591-602, 2002
    
22. Salacia reticulata and its polyphenolic constituents with lipase inhibitory and lipolytic activities have mild antiobesity effects in rats , Yoshikawa, M.;Shimoda, H.;Nishida, N.;Takada, M.;Matsuda, H. , J. Nutr. / v.132,pp.1819-1824, 2002
    

이 논문을 인용한 문헌 (0)

1. 이 논문을 인용한 문헌 없음