[논문]Effects of Green Tea Polyphenols and Fructo-oligosaccharides in Semi-purified Diets on Broilers' Performance and Caecal Microflora and Their Metabolites

Cao, B.H.; Karasawa, Y.; Guo, Y.M.

doi:10.5713/ajas.2005.85

Effects of Green Tea Polyphenols and Fructo-oligosaccharides in Semi-purified Diets on Broilers' Performance and Caecal Microflora and Their Metabolites 원문보기

Asian-Australasian journal of animal sciences, v.18 no.1, 2005년, pp.85 - 89

Cao, B.H. (College of Animal Science and Technology, China Agricultural University) , Karasawa, Y. (Faculty of Agriculture, Shinshu University) , Guo, Y.M. (College of Animal Science and Technology, China Agricultural University)

Abstract ▼ AI-Helper

This study was conducted to examine the effects of green tea polyphenols (GTP) and fructo-oligosaccharides (FOS) supplement on performance, counts of caecal microflora and its metabolites production. In female broiler chickens fed on semi-purified diets from 28 to 42 d of age, dietary green tea polyphenols (GTP) and fructo-oligosaccharides (FOS) significantly reduced mortality (p<0.05). Dietary GTP significantly decreased the total count of caecal microflora, each colonic population count and caecal flora metabolites contents when compared to other groups (p<0.05). Dietary FOS did not influence the total count of caecal flora but it selectively increased Bifidobacteri and Eubacteria counts (p<0.05) and decreased the count of other microflora and concentrations of caecal phenols and indole (p<0.0.5). These results suggest that GTP and FOS in semi-purified diets can decrease mortality and change the caecal colonic flora population, but GTP shows antibiotic-like effects of non-selectively decreasing all colonic flora and then metabolites, and FOS acts selectively by increasing profitable microflora and decreasing production of caecal microflora metabolites besides volatile fatty acids.

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문제 정의

The purpose of our experiments is to investigate the effects of GTP and FOS supplementation on performance and counts and metabolites of caecal microflora in female broiler chickens fed on semi-purified diets.

제안 방법

39 MJ/kg) and housed in a semi-open henhouse. The selected birds were assigned randomly to control, GTP and FOS groups were housed in same semiopen henhouse which was disinfected adequately for a concrete floor, and supplied with wood shaving and an automatic feeder and water lines. Each group was averagely assigned to 6 treatments for 250 birds/treatment.

대상 데이터

The birds were supplied almost equal amounts of dietary CP and ME in all groups. The experimental diets were pelleted, crumbled and stockpiled in tanks.
With an average body weight of 1,152±15 g 4,500 female commercial broilers (Arbor Acre) were selected at 28 d of age from 10,000 birds fed on commercial feed (CP 200 g/kg, AME 13.39 MJ/kg) and housed in a semi-open henhouse.

데이터처리

Data were subjected to analysis of variance using SPSS Ver.11.0 (SPSS Institute, 2001) and means compared using the Tukey-Kramer method when the F test showed significance at p<0.05.

이론/모형

Measurement of other metabolites was carried out by the method described by Yoshihara (1981) using gas chromatography (Shimatsu, type GC-14C, Japan; column: Shimadsu, type 17% Silicone SE-30, 2.0 mx3 mm, Japan) under column temperature 152°C and pressure of N2 1.0 fkg/cm2, H2 1.5 fkg/cm2 and air 1.0 fkg/cm2.
Measurement of other metabolites was carried out by the method described by Yoshihara (1981) using gas chromatography (Shimatsu, type GC-14C, Japan; column: Shimadsu, type 17% Silicone SE-30, 2.0 mx3 mm, Japan) under column temperature 152℃ and pressure of N2 1.0 fkg/cm2, H2 1.5 fkg/cm2 and air 1.0 fkg/cm2.
The composition of volatile fatty acids (VFA) was determined by the method of Koh et al. (1997): to 0.3 g of fresh caecal contents was added with 0.3 ml distilled water, then 0.2 ml, 40 mM crotonic acid standard solution. The mixed liquid was centrifuged at 10,000 rpm, for 10 minutes at 0°C.

성능/효과

The FOS supplement gave significantly lower counts of Peptococcaceae, Lecithinase-positive Clostridia, Streptococci, Staphylococci and Bacilli than the control treatment (p<0.05), but significantly greater counts of Bifidobacteria and Eubacteria (p<0.05).
The concentrations (Table 4) of acetic acid, butyric acid, propionic acid, valeric acid, phenol and indole of the GTP group were significantly lower than those of the other groups (p<0.05), and cresol and ethyl phenol values were lower than those of the control group (p<0.05).
The results observed in the present study lasting two weeks were obtained in a situation in which birds experienced stresses such as feed changes, prohibition of veterinary drugs and ingestion of GTP and FOS. In this situation, intestinal changes in the microflora community might not have achieved equilibrium; hence it is necessary to investigate the effects of longtime supplementation and their mechanism.
05) than the other groups (Table 3). This was due to the counts of Bifidobacteria, Bacteroidaceae, Peptococcaceae and Lactobacilli in the GTP group being lower than those of other groups, the count ofEubacteria ofthe GTP group being lower than that of the FOS group, and the counts of Lecithinase-positive Clostridia, Streptococci, Staphylococci and Bacilli in the GTP group being lower than those ofthe control group. The FOS supplement gave significantly lower counts of Peptococcaceae, Lecithinase-positive Clostridia, Streptococci, Staphylococci and Bacilli than the control treatment (p<0.
05) than the other groups (Table 3). This was due to the counts of Bifidobacteria, Bacteroidaceae, Peptococcaceae and Lactobacilli in the GTP group being lower than those of other groups, the count ofEubacteria ofthe GTP group being lower than that of the FOS group, and the counts of Lecithinase-positive Clostridia, Streptococci, Staphylococci and Bacilli in the GTP group being lower than those ofthe control group. The FOS supplement gave significantly lower counts of Peptococcaceae, Lecithinase-positive Clostridia, Streptococci, Staphylococci and Bacilli than the control treatment (p<0.

참고문헌 (32)

Bao, T. Z., N. Taneja, D. P. Loder, D. P., Balentine and A. H. Conney. 1998. Effects of tea polyphenols and flavonoids on liver microsomal glucuronidation of estradiol and estrone. J. Steroid Biochem. Molecular Biol. 64:207-215.
Chio, K. H., H. Namkung and I. K. Paik. 1994. Effects of dietary fructooligosaccharides on the suppression of intestinal colonization of Salmonella typhimurium in broiler chickens. Korean J. Anim. Sci. 36:271-284.
Giordani, G., A. Meluzzi, C. Cristofori, F. Sirri and F. Calini. 1994. Nutritional control of body fat in broiler chickens. Zootec. Nutr. Anim. 20:159-169.
Gonzales, E., J. Buyse, M. M. Loddi, T. S. Takita, N. Buys and E. Decuypere. 1998. Performance, incidence of metabolic disturbances and endocrine variables of food-restricted male broiler chickens. Br. Poult. Sci. 39:671-678.
Gonzalez, A. J. M., O. M. E. Suarez, M. A. Pro and C. C. Lopez. 2000. Feed restriction and salbutamol to control ascites syndrome in broilers: 1. Productive performance and carcass traits. Agrociencia. 34:283-292.
Hara, H., N. Orita, S. Hatano, H. Ichikawa, Y. Hara, N. Matsumoto, Y. Kimura, A. Terada and T. Mitsuoka. 1995. Effect of tea polyphenols on fecal flora and fecal metabolic products of pigs. J. Vet. Med. Sci. 57:45-49.
Hidaka, H., T. Edita, T. Takazawa, T. Tokunaga and Y. Tashiro. 1986. Effects of fructooligosaccharides on intestinal flora and human health. Bifidobact. Microflo. 5:37-50.
Hong, J. W., I. H. Kim, O. S. Kwon, B. J. Min, W. B. Lee and K. S. Shon. 2004. Influence of plant extract supplementation on performance and blood characteristics in weaned pigs. Asian-Aust. J. Anim. Sci. 17(3):374-378.
Koh, K., Y. Karasawa and N. Miyano. 1997. Effects of dietary granularity of Japanese oak leaf on nutrient utilization and caecal fermentation in grouse. J. Facul. Agric. Shinshu Univ. 34:11-18.
Leeson, S. and J. D. Summers. 1997. Commercial Poultry Nutrition: Chapter 5: Feeding programs for broilers. Univ. Books Ontario, Canada. 207-254.
Mitsuoka, T., H. Hidaka and T. Eida. 1987. Effect of fructooligosaccharides on intestinal microflora. Nahrung. 31:5-6, 427-436.
Mitsuoka, T., K. Ohno, Y. Benno, K. Suzuki and K. Nanba. 1976. Die faekalflora bei menschen: 4. Mitteilung: vergleich des entwickelten verfaherns mit bischerigen blichen verfaherns zur darmflora analyse. Zentr. Bakter. Parasitenk. Infek. Hygi. Abteil.1, Origin. A234:219-233.
Mohammad, J. 2002. Fructooligosaccharide and diarrhea. Biosci. Microflo. 21:31-34.
Monsan, P. F. and F. Paul. 1995. Oligosaccharide feed additives. In Biotechnology in Animal Feeds and Feeding (Ed. R. J. Wallace and A. Chesson), VCH Verlagsgesellschaft, Weinheim and New York, pp. 233-245.
Oku, T. 1986. Metabolism of new sweetener fructooligosaccharide (Neosugar？) and its application. J. Nutr. (Jap.). 44:291-306.
Okumura, J., M. Furuse, T. Kawamura, K. Toyoshima, M. Sugawara, T. Suzuki, G. Seo and H. Soga. 1994. Effects of glucooligosaccharide and biobacteria on egg production rate and cecal bacterial population in the chicken. Jpn. Poult. Sci. 31:189-194.
Oyarzable, O. A. and D. E. Conner. 1996. Application of direct fed microbial bacteria and fructooligosaccharides for Salmonella control in broilers during feed withdrawal. Poult. Sci. 75:186-190.
Pearson, D. A., E. N. Frankel, R. Aeschbach and J. B. German. 1998. Inhibition of endothelial cell mediated low-density lipoprotein oxidation by green tea extracts. J. Agric. Food Chem. 46:1445-1449.
Ryu, E. 1980. Prophylactic effect of tea on pathogenic microorganism infection to human and animal. (1) Growth inhibitive and bactericidal effect of tea on food poisoning and other pathogenic enterobacterium in vitro. Intl. J. Zoono. 7:164-170.
Samarasinghe, K., C. Wenk, K. F. S. T. Silva and J. M. D. M. Gunasekera. 2003. Turmeric (Curcuma longa) root powder and mannanoligosaccharides as alternatives to antibiotics in broiler chicken diets. Asian-Aust. J. Anim. Sci. 10:1495-1500.
Sano, M., Y. Takahashi, K. Yoshino, K. Shimoi, Y. Nakamura, I. Tomita, I. Oguni and H. Konomoto. 1995. Effect of tea (Camellia sinensis L.) on lipid peroxidation in rat liver and kidney: a comparison of green and black tea feeding. Biol. Pharmaceutic. Bul. 18:1006-1008.
Tamura, M., H. Suzuki, K. Hirayama and K. Itoh. 1997. Effects of guar gum and fructooligosaccharides on plasma lipids and cecal short-chain fatty acids in adult mice. J. Clin. Biochem. Nutr. 23:131-137.
Terada, A., H. Hara, K. Ikegame, M. Sasaki and T. Mitsuoka. 1994a. Recommended method of enumeration of lecithinasepositive clostridia in human feces. Bifidobact. Microflo. 13:29-32.
Terada, A., H. Hara, S. Nakajyo, H. Ichikawa, Y. Hara, K. Fukai, Y. Kobayashi and T. Mitsuoka. 1993. Effect of supplements of tea polyphenols on the caecal flora and caecal metabolites of chicks. Microb. Ecolo. Health Dis. 6:3-9.
Terada, A., H. Hara, J. Sakamnoto, N. Sato, S. Takagi, T. Mitsuoka, R. Mino, K. Hara, I. Fujimori and T. Yamada. 1994b. Effects of dietary supplementation with lactosucrose (4G-beta-Dgalactosylsucrose) on cecal flora, cecal metabolites, and performance in broiler chickens. Poult. Sci. 73:1663-1672.
Villiers, W. J. S., C. J. McClain, G. W. Varilek and F. J. Yang. 1998. Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model. J. Nutr. 128:2334-2340.
Waldroup, A. L., J. T. Skinner, R. E. Hierholzer and P. W. Waldroup. 1993. An evaluation of fructooligosaccharide in diets for broiler chickens and effects on salmonellae contamination of carcasses. Poult. Sci. 72:643-650.
Weisburger, J. H., J. R. Hosey, E. Larios, B. Pittman, E. Zang, Y. Hara and G. Cheraux. 2001. Investigation of commercial MitoLife as an antioxidant and antimutagen. Nutr. 17:322-325.
Yang, C. J., I. Y. Yang, D. H. Oh, I. H. Bae, S. G. Cho, I. G. Kong, D. Uuganbayar, I. S. Nou and K. S. Choi. 2003. Effect of green tea by-product on performance and body composition in broiler chicks. Asian-Aust. J. Anim. Sci. 16:867-872.
Yokozawa, T., T. Nakagawa and K. Kitani. 2002. Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J. Agric. Food Chem. 50:3549-3552.
Yoshihara, I. 1981. Isothermal gas chromatographic analysis of putrefactive products in gastrointestinal contents and urine using the same dual column system. Agric. Biollo. Chem. 45:1873-1875.
Zhang, A. Q., P. T. Chan, Y. S. Luk, K. K. Ho and Z. Y. Chen. 1997. Inhibitory effect of jasmine green tea epicatechin isomers on LDL-oxidation. J. Nutr. Biochem. 8:334-340.

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