[논문]Association of SNP Marker in IGF-I and MYF5 Candidate Genes with Growth Traits in Korean Cattle

Chung, E.R.; Kim, W.T.

doi:10.5713/ajas.2005.1061

Association of SNP Marker in IGF-I and MYF5 Candidate Genes with Growth Traits in Korean Cattle 원문보기

Asian-Australasian journal of animal sciences, v.18 no.8, 2005년, pp.1061 - 1065

Chung, E.R. (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University) , Kim, W.T. (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University)

Abstract ▼ AI-Helper

Growth rate is one of the economically important quantitative traits that affect carcass quantity in beef cattle. Two genes, bovine insulin-like growth factor I (IGF-I) and myogenic factor 5 (MYF5), were chosen as candidate genes for growth traits due to their important role in growth and development of mammals. The objectives of this study were to determine gene-specific single nucleotide polymorphism (SNP) markers of the IGF-I and MYF5 positional candidate genes and to investigate their associations with growth traits in Korean cattle. Genotyping of the SNP markers in these candidate genes was carried out using the single strand conformation polymorphism (SSCP) analysis. The frequencies of A and B alleles were 0.72 and 0.28 for IGF-I gene and 0.39 and 0.61 for MYF5 gene, respectively, in Korean cattle population examined. The gene-specific SNP marker association analysis indicated that the SNP genotype in IGF-I gene showed a significant association (p<0.05) with weight at 3 months (W3), and cows with AB genotype had higher W3 than BB genotype cows. The SNP genotype of MYF5 gene was found to have a significant effect (p<0.05) on the weight at 12 months (W12) and average daily gain (ADG), and cows with BB and AB genotypes had higher W12 and ADG compared with cows with AA genotype, respectively. However, no significant association between the SNP genotypes and any other growth traits was detected. The gene-specific SNP markers in the IGF-I and MYF5 candidate genes may be useful for selection on growth traits in Korean cattle.

주제어

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문제 정의

, 1999). In this study, we examined the association between the two SNP marker genotypes of IGF-I and MYF5 candidate genes and the growth traits in Korean cattle. In bovine, a SNP (T to C transition) was detected at 512 bp 5’ to the first codon ofthe first exon of the IGF-I gene (Ge et al.
Therefore, the IGF-I and MYF5 genes are important candidate genes for the identification of genetic markers for growth and carcass traits in livestock. The objectives of this study were to determine gene-specific single nucleotide polymorphisms (SNP) of these two candidate genes and to investigate their associations to growth traits in Korean cattle.

제안 방법

Using the PCR-SSCP analysis, we detected two SNP allelels in IGF-I and MYF5 genes of Korean cattle, respectively. Our results showed that PCR-SSCP analysis is useful technique to screen SNP genotype of candidate genes and can be used instead ofRFLP and sequence analysis.

대상 데이터

The population of Korean cattle sampled in this study was taken at the Hanwoo Experiment Station of National Livestock Research Institute in Rural Development Administration of Korea. Data were collected from a total of 280 cows registered in the official performance-testing program.

데이터처리

Allele frequencies at the two candidate loci were calculated by simple gene counting method. HardyWeinberg equilibrium in a population was tested by comparing expected and observed genotype frequencies using a chi-square test. The PROC GLM procedure of SAS (SAS, Inst.
Allele frequencies at the two candidate loci were calculated by simple gene counting method. HardyWeinberg equilibrium in a population was tested by comparing expected and observed genotype frequencies using a chi-square test. The PROC GLM procedure of SAS (SAS, Inst.
Additive genetic effects were estimated by pair-wise comparison of the two homozygous genotypes and the dominance effects were calculated as the deviation of the heterozygote effect from the average of the two homozygous genotypes. The estimated effects were tested for significance using the t-test.

이론/모형

HardyWeinberg equilibrium in a population was tested by comparing expected and observed genotype frequencies using a chi-square test. The PROC GLM procedure of SAS (SAS, Inst. Inc., Cary NC) was used to test the association between SNP marker genotypes of the IGF-I or MYF5 candidate gene and growth traits. The linear model used was as follows:

성능/효과

Additive genetic effects were estimated by pair-wise comparison of the two homozygous genotypes and the dominance effects were calculated as the deviation of the heterozygote effect from the average of the two homozygous genotypes. The estimated effects were tested for significance using the t-test.
An association study using more SNP of MYF5 including SNP in the promoter and exon regions and further functional tests of candidate genes may lead to the identification of causative mutation(s) in the gene(s) that controls growth traits in beef cattle. Consequently, our results indicate that the IGF-I and MYF5 are candidate genes that influences some growth traits in Korean cattle and these candidate genes could be used in a marker assisted selection program to improve growth traits in Korean cattle. Further investigations are needed in other populations of Korean cattle to verify the associated effects of the gene-specific SNP marker, as well as the effect ofthe other DNA polymorphisms in positional candidate genes.

후속연구

Consequently, our results indicate that the IGF-I and MYF5 are candidate genes that influences some growth traits in Korean cattle and these candidate genes could be used in a marker assisted selection program to improve growth traits in Korean cattle. Further investigations are needed in other populations of Korean cattle to verify the associated effects of the gene-specific SNP marker, as well as the effect ofthe other DNA polymorphisms in positional candidate genes.
Consequently, our results indicate that the IGF-I and MYF5 are candidate genes that influences some growth traits in Korean cattle and these candidate genes could be used in a marker assisted selection program to improve growth traits in Korean cattle. Further investigations are needed in other populations of Korean cattle to verify the associated effects of the gene-specific SNP marker, as well as the effect ofthe other DNA polymorphisms in positional candidate genes.

참고문헌 (22)

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상세보기
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