Zhang, J.H.
(Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture College of Animal Science and Veterinary Medicine, Huazhong Agricultural University)
,
Xiong, Y.Z.
(Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture College of Animal Science and Veterinary Medicine, Huazhong Agricultural University)
,
Deng, C.Y.
(Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture College of Animal Science and Veterinary Medicine, Huazhong Agricultural University)
Correlation of microsatellite heterozygosity with performance or heterosis was reported in wild animal populations and domestic animal populations, but the correlation with heterosis in a crossbreeding F$_1$ pig population remained uncertain. To explore this, we had random selected and ma...
Correlation of microsatellite heterozygosity with performance or heterosis was reported in wild animal populations and domestic animal populations, but the correlation with heterosis in a crossbreeding F$_1$ pig population remained uncertain. To explore this, we had random selected and mated Yorkshire${\times}$Meishan (F, n = 82) and their reciprocal (G, n = 47) to F$_1$, and used the two straightbreds as control groups (Yorkshire = 34, Meishan = 55), and observed the heterosis of birth weight (BWT), average daily gain (ADG) and feed and meat ratio (FMR). Two Kinds of measurement-individual heterozygosity (IH) and individual mean d$^2$ (lg value, ID) were used as index of heterozygosity and variance from 39 microsatellite marker loci to perform univariate regression analysis against heterosis. We detected significant correlation of IH with BWT in all of F$_1$ (F+G) and in F. We observed significant correlation of ID with ADG in all of F$_1$ (F+G), and with FMR in all of F$_1$ (F+G) and in F. There was significant maternal effect on heterosis, which was indicated by significant difference of means and distribution of heterosis between F and G. This difference was consistent with distributions of IH and ID, and with difference of means in F and G. From this study, it would be suggested that the two kinds of genetic index could be used to explore the genetic basis of heterosis in crossbreeding populations but could not determine which is better.
Correlation of microsatellite heterozygosity with performance or heterosis was reported in wild animal populations and domestic animal populations, but the correlation with heterosis in a crossbreeding F$_1$ pig population remained uncertain. To explore this, we had random selected and mated Yorkshire${\times}$Meishan (F, n = 82) and their reciprocal (G, n = 47) to F$_1$, and used the two straightbreds as control groups (Yorkshire = 34, Meishan = 55), and observed the heterosis of birth weight (BWT), average daily gain (ADG) and feed and meat ratio (FMR). Two Kinds of measurement-individual heterozygosity (IH) and individual mean d$^2$ (lg value, ID) were used as index of heterozygosity and variance from 39 microsatellite marker loci to perform univariate regression analysis against heterosis. We detected significant correlation of IH with BWT in all of F$_1$ (F+G) and in F. We observed significant correlation of ID with ADG in all of F$_1$ (F+G), and with FMR in all of F$_1$ (F+G) and in F. There was significant maternal effect on heterosis, which was indicated by significant difference of means and distribution of heterosis between F and G. This difference was consistent with distributions of IH and ID, and with difference of means in F and G. From this study, it would be suggested that the two kinds of genetic index could be used to explore the genetic basis of heterosis in crossbreeding populations but could not determine which is better.
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문제 정의
China (G2000016105) and National High Technology Development Project. The authors also gratefully acknowledge the teachers and postgraduate students of the Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, and the Swine Breeding Center of China for their cooperation.
The present study was intended to investigate the extent of correlation between marker heterozygosity and heterosis in pig population. We found several instances of significant effect of genetic variation (both IH and ID) on heterosis of three traits in this study, all of them were positive correlation, although the correlation was low.
제안 방법
In this paper, we attempt to examine the relationship between molecular marker heterozygosity and heterosis of two main growth traits and birth weight by two different genetics indexes-individual heterozygosity and individual mean d2 in F1 population from YorkshirexMeishan and its reciprocal. This two different measurement of heterozygosity had been used in several papers about animal fitness (Health et al.
The pig population had been raised at a herd with the same equipment and with the same ordinary food standard. Variables measured in this study were birth weight (BWT), average daily gain (ADG), feed and meat ratio (FMR). Birth weight was recorded within 24 h after birth.
대상 데이터
Thirty nine microsatellites on SSC4, SSC6, SSC7, SSC8, and SSC13 were selected based on the USDA- MARC Genome Database map (http://www.genome.iastate. edu/maps/), on which the loci position of the SSR was picked out by the interval of about 20-30 cM, and not having too many or less number of alleles so as to be apt to scoring. All of the microsatellite DNA primer was presented by US Pig Genome Coordinator (Max F.
데이터처리
The two measurements were used for calculating the correlations of heterozygosity and variance with heterosis by univariate regression. The analysis was carried out with REG procedure of SAS software, and the results were presented by tables and figures of linear regression.
The two measurements were used for calculating the correlations of heterozygosity and variance with heterosis by univariate regression. The analysis was carried out with REG procedure of SAS software, and the results were presented by tables and figures of linear regression.
성능/효과
Genotype score of individual was inferred by their parents' at the same polyacrylamide gels to avoid redundant (to expel from disturbing of nonspecific band by referring to the parental genotypes), the different microsatellite DNA fragment at the same loci on different polyacrylamide gels was distinguished by standard DNA marker (pBR322/Msp I). Allele size of microsatellite PCR amplification production was determined by method of Rickwood and Hames (1982) and referred to the standard DNA marker, so there was some error, but it was consistent and identical in all population, and the results was credible in this study.
Among the 39 microsatellite DNA markers used in this study, considerable allelic variations were observed in allele size range, as well as in number of alleles and heterozygosity across all of Fi (Table 2). Average observed heterozygosity was range from 0.
Highly significant (p<0.01) differences in heterosis of birth weight and average daily gain was observed between F and G, progeny from G has better performance and heterosis than from F, but the significant difference was not found for FMR, although there was better heterosis in progeny from G than from F (Table 1). The highest heterosis (22%) was observed in average daily gain in G, and this heterosis was best (15.
01) differences in heterosis of birth weight and average daily gain was observed between F and G, progeny from G has better performance and heterosis than from F, but the significant difference was not found for FMR, although there was better heterosis in progeny from G than from F (Table 1). The highest heterosis (22%) was observed in average daily gain in G, and this heterosis was best (15.5%) in all of Fi (F+G) among the three traits.
참고문헌 (32)
Behl, R., R. Kaul, N. Sheoran, J. Behl, M. S. Tantia and P. K. Vijh. 2002. Genetic identity of two indian pig types using microsatellite markers. Anim. Gene. 33:158-159.
Bidanel, J. P. and M. Rothschild. 2002. Current status of quantitative trait locus mapping in pigs. Pig News and Information 23:39N-54N.
Blanchfield, P. J., M. S. Ridgway and C. C. Wilson. 2003. Breeding success of male brook trout (Salvelinus fontinalis) in the wild. Molecular Ecology 12:2417-2428.
Bohn, M. Frisch. 2003. Genetic distance based on simple sequence repeats and heterosis in tropical maize population. Crop Sci. 43:1275-1282.
Bruce, A. B. 1910. The mendelian theory of heredity and augmentation of vigor. Sci. 32:627-628.
Coltman, D. W., W. D. Bowen and J. M. Wright. 1998. Birth weight and neonatal survival of harour seal pups are positively correlated with genetic variation measured by microsatellites. Proc. R. Soc. Lond B Biol. Sci. 265:803-809.
Coulson, T. N., J. M. Pemberton, S. D. Albon, M. Beaumont, T. C. Marshall, J. Slate, F. E. Guinness and T. H. Clutton-Brock. 1998. Microsatellites reveal heterosis in red deer. Proc. R. Soc. Lond. B 265:489-495.
Curik, I., P. Zechner, J. Solkner, R. Achmann, I. Bodo, P. Dove, T. Kavar, E. Marti and G. Brem. 2003. Inbreeding, microsatellite heterozygosity and morphological traits in lipizzan horses. J. Hered. 94:125-132.
Dieringer, D. and C. Schlotterer. 2003. Microsatellite analyzer (MSA) - a platform independent analysis tool for large microsatellite data sets. Molecular Ecology Notes 3:167-169.
Falconer, D. S. and T. F. C. Mackay. 1996. Introduction to Quantitative Genetics. Fourth edition, Longman Group Ltd. Press, p. 259.
Gavora, J. S., R. W. Fairfull, B. F. Benkel, W. J. Cantwell, J. R. Chambers. 1996. Prediction of heterosis from DNA fingerprints in chickens. Genetics 144:777-784.
Hansson, B., S. Bensch, D. Hasselquist and M. Akesson. 2001. Microsatellite diversity predicts recruitment of sibling great reed warblers. Proc. R. Soc. Lond. B. 268:1287-1291.
Health, D. D., C. A. Bryden, J. M. Shrimpton, G. K. Iwama, J. Kelly and J. W. Heath. 2002. Relationships between heterozygosity, allelic distance (d2), and reproductive traits in chinook salmon, Oncorhynchus tshawytscha Can. J. Fish. Aquat. Sci. 59:77-84.
Jiang, X. P., Y. Z. Xiong, G. Q. Liu, C. Y. Deng and Y. C. Qu. 2003. The effect of individual heterozygosity of gene on growth performance in pig. Acta Genetica Sinica 30:82-87.
Laval, G., N. Iannuccelli, C. Legault, D. Milan, M. A. M. Groenen, E. Giuffra, L. Andersson and Nissen P. H. 2000. genetic diversity of eleven European pig breeds. Genet. Sel. Evol. 32:187-203
Li, C. C., Z. G. Wang, B. Liu, S. L. Yang, Z. M. Zhu, B. Fan, M. Yu, S. H. Zhao and K. Li. 2004. Evaluation of the genetic relationship among ten chinese indigenous pig breeds with twenty-six microsatellite markers. Asian-Aust. J. Anim. Sci. 17:441-444.
Mallet, A. L., E. Zouros, K. E. Gartner-Kepkay, K. R. Freeman and L. M. Dickie. 1985. Larval viability and heterozygote deficiency in populations of marine bivalves: evidence from pair matings of mussels. Marine Biology 87:165-172.
Nicolas, B., S. Launey, Y. Naciri-Graven and F. Bonhomme. 1998. Early effect of inbreeding as revealed by microsatellite analyses on ostrea edulis larvae. Genetics 148:1893-1906.
Reif, J. C., A. E. Melchinger, X. C. Xia, M. L. Warburton, D. A. Hoisington, S. K. Vasal, G. Srinivasan, M. Bohn and M. Frisch. 2003. Genetic distance based on simple sequence repeats and heterosis in tropical maize mopulation. Crop Sci. 43:1275-1282.
Rickwood, D. and B. D. Hames. 1982. Gel Electrophoresis of Nucleic Acids: A Practical Approach. IRL Press Limited (Translated to Chinese by Zhao Dajian et al., Science Press, Beijing, 1989, pp. 40-41).
Shikano, T. and N. Taniguchi. 2003. DNA markers for estimation of inbreeding depression and heterosis in the guppy Poecilia reticulate. Aquaculture Research 34:905-911.
Slate, J. L., E. B. Kruuk, T. C. Marshall, J. M. Pemberton and T. H. Clutton-brock. 2000. Inbreeding depression influences lifetime breeding success in a wild population of red deer (Cervus elaphus). Proc. R. Soc. Lond. B 267:1657-1662.
Shull, G. H. 1908. The composition of a field of maize. Am. Breed Assoc. 4:298-301.
Sun, C. Q., T. B. Jiang, Y. C. Fu and X. K. Wang. 2002. Indica？japonica differentiation of paddy rice and its relationship with heterosis. Plant Breeding 121:330-337.
Wang, X., H. H. Cao, S. M. Geng and H. B. Li. 2004. Genetic diversity of 10 indigenous pig breeds in China by using microsatellite markers. Asian-Aust. J. Anim. Sci. 17:1219-1222.
Wu, X. L., L. Xun and Merete Fredholm. 2001. The research of correlation of genomic heterzygosity with economic traits in pig population under successive inbred by microsatellite DNA marker. Acta Genetic Sinica 28:20-28.
Xiong, Y. Z. and C. Y. Deng. 1999. Principle and method of Swine Testing. Chinese Agricultural Press, Beijing
Yu, S. B., J. X. Li, C. G. Xu, Y. F. Tan, Y. J. Gao, X. H. Li, Qifa Zhang and M. A. Saghai Maroof. 1997. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc. Natl. Acad. Sci. USA 94:9226-9231.
Zhang, Qifa, Y. J. Gao, S. H. Yang, R. A. Ragab, M. A. Saghai Maroof and Z. B. Li. 1994. A diallel analysis of heterosis in elite hybrid rice based on RFLPs and microsatellite. Theor. Appl. Genet. 89:185-192.
Zhang, Qifa, Z. Q. Zhou, G. P. Yang, C. G. Xu, K. D. Liu and M. A. Saghai Maroof. 1996. Molecular marker heterozygosity and hybrid performance in indica and japonica rice. Theor. Appl. Genet. 96:1218-1224.
Zouros, E. and D. W. Foltz. 1984. Possible explanations of heterozygote deficiency in bivalve molluscs. Malacologia 25:583-591.
Zuo, B., Y. Z. Xiong, Y. H. Su, C. Y. Deng, R. Zheng and S. W. Jiang. 2003. Mapping quantitative trait loci for meat quality on pig chromosome 3, 4 and 7. Asian-Aust. J. Anim. Sci. 16:320-324.
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