[논문]Effect of Removal of Follicles through Repeated Transvaginal Follicle Aspiration on Subsequent Follicular Populations in Murrah Buffalo (Bubalus bubalis

Akshey, Y.S.; Palta, P.; Manik, R.S.; Vivekananad, Vivekananad; Chauhan, M.S.

doi:10.5713/ajas.2005.632

Effect of Removal of Follicles through Repeated Transvaginal Follicle Aspiration on Subsequent Follicular Populations in Murrah Buffalo (Bubalus bubalis 원문보기

Asian-Australasian journal of animal sciences, v.18 no.5, 2005년, pp.632 - 636

Akshey, Y.S. (Animal Biotechnology Centre, National Dairy Research Institute) , Palta, P. (Animal Biotechnology Centre, National Dairy Research Institute) , Manik, R.S. (Animal Biotechnology Centre, National Dairy Research Institute) , Vivekananad, Vivekananad (Animal Biotechnology Centre, National Dairy Research Institute) , Chauhan, M.S. (Animal Biotechnology Centre, National Dairy Research Institute)

Abstract ▼ AI-Helper

This study was conducted to investigate the effects of removal of all ovarian follicles through repeated transvaginal follicle aspiration (TVFA) on the subsequent follicular populations in buffaloes. This information is crucial for determining the optimum time interval between successive aspirations for recovering oocytes from live buffaloes through Transvaginal Oocyte Retrieval (TVOR). The oestrus of cycling buffaloes (n=5) were synchronized by a single PGF injection schedule. All the ovarian follicles were removed once every 7 days for 6 weeks through TVFA, starting from Day 7 of the oestrous cycle (Day 0 = day of oestrus). The number and size of individual ovarian follicles was recorded at Day 3 and Day 5 (Day 0 = day of TVFA) through transrectal ultrasonography. The follicles were classified on the basis of their diameter as small (3-5 mm), medium (6-9 mm) and large ($\geq$10 mm). There was no difference in the number of small and medium follicles, and the number of total follicles between Day 3 and Day 5. However, the number of large follicles was significantly higher (p<0.05) at Day 5 than that at Day 3. There was a significant (p<0.05) decrease in the proportion of small follicles and an increase (p<0.05) in the proportion of large follicles from Day 3 to Day 5, with no change in the proportion of medium follicles. The number of total follicles at Day 3 or Day 5 did not differ during the 6 TVFA sessions. It can be concluded that an interval of 3 days is more suitable than that of 5 days between successive aspirations for recovering oocytes through TVOR in a twice weekly schedule and that repeated removal of follicles through TVFA does not adversely affect the number of total follicles 3 or 5 days after TVFA.

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제안 방법

These were maintained under general feeding and management conditions at the National Dairy Research Institute (NDRI) animal farm. The experiment was completed between February and April.
The number and size of individual ovarian follicles was recorded on Day 3 and Day 5 after each TVFA session through transrectal ultrasonography. The study continued for a total of 6 TVFA sessions.

대상 데이터

m injection of 25 mg of Lutalyse, a PGF?a analog (Dinoprost tromethamin). Five out of the total 11 animals, which exhibited oestrus 72 h after PGF injection were subjected to transvaginal follicular aspiration (TVFA). Occurrence of oestrus was confirmed by rectal palpation and by behavioral oestrus signs.
Five out of the total 11 animals, which exhibited oestrus 72 h after PGF injection were subjected to transvaginal follicular aspiration (TVFA).

데이터처리

The number of total follicles at Days 3 and 5 were compared between different weeks by one way ANOVA after log transformation of data.

성능/효과

, 1996). Our results are in agreement with this report in which the authors reported that in Italian Mediterranean buffaloes subjected to TVFA every 3 to 5 days for 2 or 4 months, the number of total follicles was similar 3, 4 and 5 days after removal of all follicles through TVFA. In a similar study, Boni et al.
Our results suggest that that there was no effect of once weekly repeated TVFA on the number of total follicles at day 3 or day 5 during the 6-week period.
Garcia and Salahheddine (1998) who carried out daily ultrasonographic examination of ovaries in cows subjected to TVFA observed that emergence of new follicular growth was initiated within 2 days after the puncture procedure and suggested that TVFA induces and synchronizes subsequent follicular wave emergence. The results of our study confirm these results and suggest that the new follicular wave started after removal of all follicles during previous TVFA reaches its full numerical development within 3 days of TVFA and that there is no further increase in the number of follicles from day 3 to day 5 after TVFA.
The results of our study confirm these results and suggest that the new follicular wave started after removal of all follicles during previous TVFA reaches its full numerical development within 3 days of TVFA and that there is no further increase in the number of follicles from day 3 to day 5 after TVFA.
The results of the present study suggest that there is no difference in the number of total follicles present 3 or 5 days after removal of all follicles through TVFA.

참고문헌 (25)

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상세보기
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DOI : 10.5713/ajas.2005.632 [무료]
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