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논문 상세정보

Abstract

Large White, an introduced European pig breed, and Meishan, a Chinese indigenous pig breed, were hybridized directly and reciprocally and a total of 260 pigs, including purebreds, Large White and Meishan, and their hybrids, White${\times}$Meishan (LM) and Meishan${\times}$Large White (ML) pigs, were bred in our laboratory. The mRNA differential display PCR (DD-PCR) was used to detect the age-dependent changes of differential gene expression in backfat tissue between hybrids and parents. Some measures were taken to reduce the false positives in our experiment. Among the total of 2,686 bands obtained, 1,952 bands (about 72.67%) were reproducible and eight patterns (fifteen kinds) of gene expression were observed. The percentage of differentially expressed genes between hybrids and parents is 56.86% at the age of four months and 57.71% at the age of six months. This indicated that the differences of gene expression between hybrids and their parents were very obvious. U-test was used to compare the patterns of gene expression between the age of four and six months, and results showed that bands occurring in only one hybrid and bands displayed in one hybrid and one parent were significantly different at p<0.05, and bands visualized in only two hybrids were significantly different at p<0.01. These indicated that differential gene expression between hybrids and parents changed at different ages.

참고문헌 (17)

  1. Liu, G. Y., Y. Z. Xiong, C. Y. Deng, B. Zuo and J. H. Zhang. 2004. Comparison of gene expression patterns in Longissimus dorsi of pigs between the high-parent heterosis cross combination Landrace${\times}$Large White and the mid-parent heterosis cross combination Large White${\times}$Meishan. Asian-Aust. J. Anim. Sci. 17(9):1192-1196. 
  2. Smith, N. R., M. Aldersley, A. Li, A. High, T. P. Moynihan, A. F. Markam and P. A. Robinson. 1997. Autoamted differential display using a fluorescently labeled universal primer. Biotechniques. 23:274-279. 
  3. Xiong, L. Z., G. P. Yang, Q. F. Zhang and M. A. S. 1998. Relationship of differential gene expression in leaves with heterosis and heterozygosity in a rice diallel cross. Molecular Breeding 4:129-136. 
  4. Yumoto, H., H. Nakae, K. Fujinaka, S. Ebisu and T. Matsuo. 1999. Interleukin-6 (IL-6) and IL-8 Are Induced in Human Oral Epithelial Cells in Response to Exposure to Periodontopathic Eikenella corrodens. Infect Immun. 67:384-394. 
  5. Wang, D., Y. Zhang, Y. Yu, G. Y. Xu and J. Y. Li. 2004. Gene differential expression of liver tissues in crossbred versus purebred chicken and their relationship with heterosis of meat trait. 31:257-264. 
  6. Doss, R. P. 1996. Differential display without radioactivity-a modified procedure Biotechniques. 21:408-412. 
  7. Romagnoli, S., M. Maddaloni, C. Livini and M. Motto. 1990. Relationship between gene expression and hybrid vigor in primary root tips of young maize (Ed. L. Zea mays) plantlets. Theor. Appl. Genet. 80:767-775. 
  8. Mo, D. L., B. Liu, Z. G. Wang, S. H. Zhao, M. Yu, B. Fan, M. H. Li, S. L. Yang, G. X. Zhang, T. A. Xiong and K. Li. 2003. Genetic variation and genetic relationship of seventeen Chinese indigenous pig breeds using ten serum protein loci. Asian-Aust. J. Anim. Sci. 16(7):939-945. 
  9. Yamauchi, T., J. Kamon, H. Waki and Y. Terauchi. 2001. The fatderived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat. Med. 7:941-946. 
  10. Kern, P. A., M. Saghizadeh, J. M. Ong, R. J. Bosch, R. Deem and R. B. Simsolo. 1995. The expression of tumor necrosis factor in human adipose tissue. Regulation by obesity, weight loss, and relationship to lipoprotein lipase. J. Clin Invest. 95:2111-2119. 
  11. Rowe, A., J. Obeiro, C. I. Newbold and K. Marsh. 1995. Plasmodium falciparum rosetting is associated with malaria severity in Kenya. Infect Immun. 636:2323-2326. 
  12. Luheshi, G. N., J. D. Gardner, D. A. Rushforth, A. S. Loudon and N. J. Rothwell. 1999. Leptin actions on food intake and body temperature are mediated by IL-1. Luheshi.Proc. Natl. Acad. Sci. USA. 96:7047-7052. 
  13. Liang, P., W. M. Zhu, X. Y. Zhang, Z. Guo, R. O’Connell, L. Averboukh, F. L. Wang and A. B. Pardee. 1994. Differential display using one-base anchored oligo-dT primers. Nucleic Acids Res. 22:5763-5764. 
  14. Liang, P., L. Averboukh and A. B. Pardee. 1993. Distribution and cloning of enkaryotic mRNA by means of differential display: refinements and optimization. Nucleic Acids Res. 21:3269-3275. 
  15. Liang, P. and A. B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Sci. 257:967-970. 
  16. Xie, X. D., ZH F. Ni, F. R. Men, L. M. Wu, ZH. K. Wang and Q. X. Sun. 2003. Relationship between difference of gene expression in early developing seeds of hybrid versus parents and heterosis in wheat. Acta Genetica Sinica. 30:260-266. 
  17. Tsaftaris, A. S. 1995. Molecular aspects of heterosis in plants. Physiologia Plantarum. 94:362-370. 

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