[논문]Expression of Serum and Muscle Endocrine Factors at Antemortem and Postmortem Periods and Their Relationship with Pig Carcass Grade

Kim, W.K.; Kim, M.H.; Ryu, Y.H.; Ryu, Y.C.; Rhee, M.S.; Seo, D.S.; Lee, C.Y.; Kim, B.C.; Ko, Y.

doi:10.5713/ajas.2005.716

Expression of Serum and Muscle Endocrine Factors at Antemortem and Postmortem Periods and Their Relationship with Pig Carcass Grade 원문보기

Asian-Australasian journal of animal sciences, v.18 no.5, 2005년, pp.716 - 722

Kim, W.K. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Kim, M.H. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Ryu, Y.H. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Ryu, Y.C. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Rhee, M.S. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Seo, D.S. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Lee, C.Y. (Regional Animal Industry Research Center) , Kim, B.C. (Department of Animal Science, College of Life and Environmental Sciences, Korea University) , Ko, Y. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)

Abstract ▼ AI-Helper

Carcass weight and backfat thickness are primary yield grading factors. Insulin-like growth factor (IGF)-I/-II, transforming growth factor $\beta$1 (TGF-$\beta$1), and epidermal growth factor (EGF) regulate the proliferation and differentiation of cells including adipocytes. Also, interleukin (IL)-2/-6, cortisol, and dehydroepiandrosterone-sulfate (DHEA-S) are known to be related to muscle growth and fat depth. However, the relationships between endocrine factors and carcass grade have not been studied. Therefore, this study aimed to measure the concentrations of endocrine factors in serum and muscle, and to investigate the relationship of endocrine factors with carcass grade. A total of 60 crossbred gilts (Duroc${\times}$Yorkshire${\times}$Landrace) were used. Blood from the jugular vein was collected at antemortem (7 days before slaughter) and postmortem periods, and M. Longissimus was collected at 45 min and 24 h after slaughter. The concentrations of IGF-I/-II, EGF, TGF-$\beta$1, IL-2/-6, cortisol and DHEA-S were analyzed by radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA). In general, IGF and EGF concentrations in serum and muscle of grade A carcasses were found to be higher than those of grade C carcasses at antemortem and postmortem periods, whereas the pattern of TGF-$\beta$1 concentration was reversed. In particular, the concentrations of muscle IGF-I (24 h postmortem) and serum TGF-$\beta$1 (antemortem) were significantly different between grades A and C (p<0.05). The present results indicate that serum and muscle growth factors affect carcass weight and backfat thickness, and indirectly suggest the possibility that carcass grade could be predicted by expression of serum and/or muscle growth factors.

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, 1993). These studies point toward a positive relationship between EGF expression and fat deposition. Although the concentrations of serum and muscle EGF between grades A and C showed no significant differences, it is of interest that grade C, with lower backfat thickness, showed a tendency toward lower EGF expression.

제안 방법

, 2003b). For these reasons, we measured concentrations of endocrine factors in serum and muscle in order to investigate the relationship between endocrine factors and carcass grade.
Collectively, the findings of all these reports make strong implications that endocrine factors affect carcass weight and backfat thickness. Therefore, the present study was performed to measure the concentrations of endocrine factors in serum and muscle at antemortem and postmortem periods, and to investigate the relationship between endocrine factor expression and carcass grades.
To investigate the possible effect of cytokines and hormones on carcass grade, the concentrations of cytokines and hormones were measured and compared on the basis of carcass grade. Serum concentration of IL-2 in grade B was higher than that in other grades at antemortem and that of grade C at postmortem, although these difference were not significant (Table 5).
To investigate the relationship between growth factors and carcass grade, the levels of growth factors were analyzed in serum and muscle at antemortem and postmortem periods.

대상 데이터

A total of sixty 21-week-old crossbred gilts (DurocxYorkshirexLandrace) were used. Pigs were slaughtered by electrical-stunning, and exsanguinated for 5 min, and then scalded at 65℃ hot water.
A total of sixty 21-week-old crossbred gilts (DurocxYorkshirexLandrace) were used. Pigs were slaughtered by electrical-stunning, and exsanguinated for 5 min, and then scalded at 65℃ hot water.
Ater evisceration, carcasses were weighed and backfat thickness was also measured at the 11th and last thoracic vertebrae. The carcasses were graded according to the Korean Grading System for Animal Products (Table 1) and placed in the chill room.

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