• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.

참고문헌 (22)

  1. Chrenck, P., J. Huba and D. Vasicek. 2003. The relation between genetic polymorphism markers and milk yield in brown Swiss cattle imported to Slovakia. Asian-Aust. J. Anim. Sci. 16:1397-1401. 
  2. Deguchi, Y. and S. Kishimoto. 1990. Enhanced expression of the heat shock protein gene in peripheral blood mononuclear cells of patients with active systemic lupus erythematosus. Ann. Rheum. Dis. 49:893-895. 
  3. Favatier, F., L. Bornman, L. E. Hightower, E. Gunther and B. S. Polla. 1997. Variation in hsp gene expression and Hsp polymorphism: do they contribute to differential disease susceptibility and stress tolerance? Cell Stress Chaperones 2:141-155. 
  4. Holter, J. B., J. W. West and M. L. McGilliard. 1997. Predicting ad libitum dry matter intake and yield of Holstein cows. J. Dairy. Sci. 80:2188-2199. 
  5. Milner, C. M. and R. D. Campbell. 1990. Structure and expression of the three MHC-linked Hsp70 genes. Immunogenetics. 32:242-251. 
  6. Ross, T., R. Olivier and L. Monney. 1998. Bcl-2 prolongs cell survival after Bax-induced release of cytochrome. Nature 391:496-499. 
  7. Villunger, A., E. M. Michalak and M. Coultas. 2003. p53 and drug-induced apoptitic response mediated by BH3-only proteins puma and noxa. Science 302:1036-1038. 
  8. Milner, C. M. and R. D. Campbell. 1992. Polymorphic analysis of three MHC-linked Hsp70 genes. Immunogenetics 36:357-362. 
  9. Pociot, F., K. S. Ronningen and J. Nerup. 1993. Polymorphic analysis of the human MHC-linked heat shock protein 70 (HSP70-2) and HSP70-Hom genes in insulin-dependent diabetes mellitus (IDDM). Scand. J. Immunol. 338: 491-495. 
  10. Ahn, S. G. and D. J. Thiele. 2003. Redox regulation of mammalian heat shock factor 1 is essential for Hsp gene activation and protection from stress. Gene and Development 17:516-528. 
  11. Yic, C., C. M. Knudson and S. J. Korsmeyer. 1997. Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Nature 385:637-640. 
  12. Lyashko, V. N., V. K. Vikulova, V. G. Chernikov, V. I. Ivanov, K. A. Ulmasov and O. G. Zatsepina. 1994. Comparison of the heat shock response in ethnically and ecologically different human populations. Proc. Natl. Acad. Sci. USA 91:12492-12495. 
  13. Shimizu, S., K. Nomura, M. Ujihara, K. Sakamoto, H. Shibata and T. Susuki. 1996. An allele-specific abnormal transcript of the heat shock protein 70 gene in patients with major depression. Biochem. Biophys. Res. Commun. 219:745-752. 
  14. Holter, J. B., W. J. West, M. L. McGilliard and A. N. Pell. 1996. Predicting ad libitum dry matter intake and yields of Jersey cows. J. Dairy Sci. 79:912-921. 
  15. Perfettini, J. L., R. T. Kroemer and G. Kroemer. 2004. Fatal liaisons of p53 with Bax and Bak. Nature Cell Biology. 6:386-388. 
  16. Aron, Y., M. Busson, B. S. Polla, D. Dusser, A. Lockhart and E. Swierczewski. 1999. Analysis of hsp70-1 gene polymorphism in allergic asthma. Allergy 54:165-170. 
  17. Mosser, D. D., A. W. Caron and L. Bourget. 1997. Role of the human heat shock protein HSP70 in protection against stressinduced apoptosis. Mol. Cell. Biol. 17:5317-5321. 
  18. Favatier, F., M. R. Jacquier-Sarlin, E. Swierczewski and B. S. Polla. 1999. Polymorphism in the regulatory sequence of the human hsp70-1 gene does not affect heat shock factor binding or heat shock protein synthesis. Cell. Mol. Life Sci. 56:701-708. 
  19. Badola, S., T. K. Bhattacharya and T. K. Biswas. 2004. A comparison on polymorphism of Bata-lactoglobulin gene in Bos Taurus and indicine${\times}$Taurine crossed cattle. Asian-Aust. J. Anim. Sci. 17:733-736. 
  20. Silvia, E., Valtorta and Miriam R. Gallardo. 2003. Evaporative cooling for Holstein dairy cows under grazing conditions. International Journal of Biometeorology 25:59-87. 
  21. Block, C. J., C. Chase and P. J. Hansen. 2002. Inheritance of resistance of bovinepreimplantation embryos to heat shock: Relative importance of thematernal versus paternal contribution. Mol. Repro. Dev. 63:32-37. 
  22. Dhillon, V. B., S. McCallum, P. Norton, B. M. Twomey, F. Erkeller-Yuksel and P. Lydyard. 1993. Differential heat shock protein overexpression and its clinical relevance in systemic lupus erythematosus. Ann. Rheum. Dis. 52:436-442. 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일