Zimmermann, B.
(University of Hohenheim, Institute of Animal Nutrition)
,
Mosenthin, R.
(University of Hohenheim, Institute of Animal Nutrition)
,
Rademacher, M.
(Degussa AG)
,
Lynch, P.B.
(Teagasc, Moorepark Research Centre)
,
Esteve-Garcia, E.
(Department of Animal Nutrition, IRTA)
A study consisting of 3 trials was designed to assess the relative biological efficacy of DL-methionine (DL-Met) in comparison to liquid methionine hydroxy analogue (MHA-FA) in growing pigs. In trial I a basal diet was supplemented with three graded levels of DL-methionine (0.25, 0.50 and 0.75 g/kg)...
A study consisting of 3 trials was designed to assess the relative biological efficacy of DL-methionine (DL-Met) in comparison to liquid methionine hydroxy analogue (MHA-FA) in growing pigs. In trial I a basal diet was supplemented with three graded levels of DL-methionine (0.25, 0.50 and 0.75 g/kg) or liquid MHA-FA (0.0285, 0.0570 and 0.0855 g/kg) on equimolar basis. The basal diet contained 18.3% CP, 0.22% Met and 0.51% Met+Cys, which is below the methionine requirement for weaned pigs between 10 and 20 kg BW according to NRC (1998) but adequate in all other essential nutrients and energy. Using an exponential model, the efficacy of the two methionine sources was estimated from nitrogen retention data obtained in 42 piglets with an initial BW between 11.0 kg (Exp. 1) and 11.7 kg (Exp. 2). In trials II and III, with a total of 192 and 96 pigs, and with an initial BW of 10.6 and 21 kg, respectively, growth response criteria were assessed to determine if in agreement with previous studies in pigs and poultry a biological effectiveness of about 65% on average could be confirmed for liquid MHA-FA in comparison to DL-met. Based on N-retention (trial I) the biological efficacy of liquid MHA-FA on a weight-to-weight basis was calculated to be 62% relative to DL-met. Basically, these results were confirmed using growth response criteria as measures; the results of trial II and III revealed no significant differences in growth performance and feed conversion between treatments indicating that 100 parts of liquid MHA-FA can be replaced by 65 parts of DL-met.
A study consisting of 3 trials was designed to assess the relative biological efficacy of DL-methionine (DL-Met) in comparison to liquid methionine hydroxy analogue (MHA-FA) in growing pigs. In trial I a basal diet was supplemented with three graded levels of DL-methionine (0.25, 0.50 and 0.75 g/kg) or liquid MHA-FA (0.0285, 0.0570 and 0.0855 g/kg) on equimolar basis. The basal diet contained 18.3% CP, 0.22% Met and 0.51% Met+Cys, which is below the methionine requirement for weaned pigs between 10 and 20 kg BW according to NRC (1998) but adequate in all other essential nutrients and energy. Using an exponential model, the efficacy of the two methionine sources was estimated from nitrogen retention data obtained in 42 piglets with an initial BW between 11.0 kg (Exp. 1) and 11.7 kg (Exp. 2). In trials II and III, with a total of 192 and 96 pigs, and with an initial BW of 10.6 and 21 kg, respectively, growth response criteria were assessed to determine if in agreement with previous studies in pigs and poultry a biological effectiveness of about 65% on average could be confirmed for liquid MHA-FA in comparison to DL-met. Based on N-retention (trial I) the biological efficacy of liquid MHA-FA on a weight-to-weight basis was calculated to be 62% relative to DL-met. Basically, these results were confirmed using growth response criteria as measures; the results of trial II and III revealed no significant differences in growth performance and feed conversion between treatments indicating that 100 parts of liquid MHA-FA can be replaced by 65 parts of DL-met.
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제안 방법
Treatment effects were analysed as main effects, and the effect of room was included in the model as a covariate with mean weight of the pair on d 11. Single degree of freedom comparisons were carried out to compare the control (unsupplemented) treatment with the mean of the two supplemented treatments and, to compare the liquid MHA-FA diet with the DL-Met supplemented diet.
Statistical analyses : The trial was designed as a randomized complete block design with 8 blocks (4 blocks of males and 4 blocks of females) and three dietary treatments. Blocks included sex, weight and location effects.
The experimental diets were fed to pigs ranging from 29-35 kg in BW. The daily N retention showed no significant differences in response to the supplementation of DL-Met or MHA-FA which is not in agreement with the results oftrial I in this study.
대상 데이터
The animals had free access to water and feed. The trial was carried out in four rooms each holding 24 pairs/pens of pigs. Temperature was maintained at 28℃ in the first wk and reduced by 2℃ per wk to 22℃ in wk four.
데이터처리
Blocks included sex, weight and location effects. Comparisons between the three treatment groups were made using t-test procedures (SAS 1996).
Statistical analyses : The experimental data were analyzed using the GLM Procedure of SAS (1996). The LSQ-test was used to test differences between treatment means.
05. The results were subjected to nonlinear regression analysis. The efficacy of the two methionine sources was estimated from different response data using an exponential model (Y = a+bx[1 - e -(C1X1+C2X2))]), whereas Y means the response parameter, x1 the added analyzed DL-Met and x2 the added analyzed liquid MHA-FA product.
이론/모형
Statistical analyses : Statistical analysis was carried out using the GLM Procedure of SAS (1996). The pair/pen was considered as the experimental unit.
1 N HCl (Commission Directive, 1998). Supplemented liquid MHA-FA was analyzed using the method described by Naumann et al. (1976).
Statistical analyses : The experimental data were analyzed using the GLM Procedure of SAS (1996). The LSQ-test was used to test differences between treatment means. The statistical significance level was claimed at p<0.
There is a controversial discussion about the biological efficacy of DL-Met in relation to liquid MHA-FA. The results of this study support earlier findings by Schmidt (2000) who also used the N balance technique for testing the efficacy of liquid MHA-FA in relation to DL-Met. The author used pigs in the BW range from 20 to 50 kg.
성능/효과
05) at the highest level ofDL-Met and liquid MHA-FA supplementation, respectively. Consequently, the utilisation ofN was improved, which resulted in an increase (p[0.05) in N retention from 4.6 g/d in pigs fed the basal diet to 5.9 g/d when the diets with the highest level of supplemental DL-Met or liquid MHA-FA were given. However, at the lowest level of liquid MHA-FA supplementation to the basal diet there was no effect (p]0.
The results of this report are in accordance with those obtained in trial II (Figure 2) and trial III (Table 6) of the present study, confirming that 65 parts of DL-Met can replace 100 parts of liquid MHA-FA in diets for growing pigs. In both trials, the basal diet was clearly deficient in methionine, confirmed by a significant response in growth rate and feed efficiency, irrespective of the source of supplemental methionine.
Supplementation of either 65 parts of DL-Met or 100 parts ofliquid MHA-FA of a methionine-deficient basal diet resulted in a significantly (p<0.01) higher daily weight gain (501 and 488 g vs. 432 g) and improved (p<0.05) feed conversion (1.48 and 1.48 vs. 1.61) compared with the pigs of the control group. During the growth period from 20 to 30 kg BW no significant differences were found between both methionine sources for the variables that were measured.
Supplementation of either 65 parts of DL-Met or 100 parts ofliquid MHA-FA of a methionine-deficient basal diet resulted in a significantly (p<0.01) higher daily weight gain (501 and 488 g vs. 432 g) and improved (p<0.05) feed conversion (1.48 and 1.48 vs. 1.61) compared with the pigs of the control group. During the growth period from 20 to 30 kg BW no significant differences were found between both methionine sources for the variables that were measured.
05) in growth response and feed conversion between both methionine sources. The results of this report are in accordance with those obtained in trial II (Figure 2) and trial III (Table 6) of the present study, confirming that 65 parts of DL-Met can replace 100 parts of liquid MHA-FA in diets for growing pigs. In both trials, the basal diet was clearly deficient in methionine, confirmed by a significant response in growth rate and feed efficiency, irrespective of the source of supplemental methionine.
The results of this report are in accordance with those obtained in trial II (Figure 2) and trial Ⅲ (Table 6) of the present study, confirming that 65 parts of DL-Met can replace 100 parts of liquid MHA-FA in diets for growing pigs.
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