선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- However, the changes in development of gastrointestinal tract and pancreatic enzymes with age have not been extensively examined in geese. The aim of this experiment was to investigate the development of gastrointestinal tract and digestive enzymes in the pancreas of geese during the embryonic period to first 28 days after hatching
- One hundred and twenty breeding White Roman geese eggs on day 22 of incubation got from Chang-Hua animal breeding station, Taiwan Livestock Research Institute, Council of Agriculture (COA-TLRI) were used. The eggs were continuously hatched in our laboratory, and thirty eggs were sampled randomly at days 22, 24 and 26 of incubation and at hatching, respectively. The embryos were removed from the eggs and weighed.
대상 데이터
- The birds were fed with starter diet (containing 200 g protein/kg and 2,900 kcal MEn/kg) (Table 1). At 1, 3 and 7 days of age, sixteen goslings, 8 males and 8 females, were selected, respectively. In addition, eight goslings, 4 males and 4 females, at 11, 14, 21 and 28 days of age were selected, respectively.
- At 1, 3 and 7 days of age, sixteen goslings, 8 males and 8 females, were selected, respectively. In addition, eight goslings, 4 males and 4 females, at 11, 14, 21 and 28 days of age were selected, respectively. Before being sacrificed, the geese were fasted for 12 h and then fed for 3 h.
- One hundred and twenty breeding White Roman geese eggs on day 22 of incubation got from Chang-Hua animal breeding station, Taiwan Livestock Research Institute, Council of Agriculture (COA-TLRI) were used. The eggs were continuously hatched in our laboratory, and thirty eggs were sampled randomly at days 22, 24 and 26 of incubation and at hatching, respectively.
이론/모형
- All data were analyzed using the General Linear Models Procedures of SAS (SAS, 1996). Comparison of treatment means was made using a Least Squares Means test.
- All data were analyzed using the General Linear Models Procedures of SAS (SAS, 1996). Comparison of treatment means was made using a Least Squares Means test. A significance level of p<0.
- The feed constituents and nutrient compositions in the residue yolk were analyzed according to the standard procedures (AOAC, 1990). The a-amylase (EC 3.2.1.1) activity was determined according to the method of Onodera et al. (1988) using soluble starch (Wako Pure Chemical Industry Ltd., Japan) as substrate. One unit of aamylase was expressed as 1 mg glucose released per minute at 37°C.
- The entire pancreas of the embryos and goslings were removed immediately for determining the activities of amylase, trypsin, chymotrypsin and lipase during experimental period. The crude pancreatic enzymes were prepared according to the method of Kidder and Manners (1980). Samples were weighed and homogenized with a Potter Elvenhjem device (TRI-R, Instruments, Model-k41) at 4°C in a saline solution that was 4 times of the sample weight.
- (1986). The protein content of crude enzyme samples were measured according to the method of Bradford (1976).
- The trypsin and chymotrypsin activities were assayed using the method of Rick (1974) with N-a- Benzoyl-L-arginine ethyl-ester (BAEE, Sigma B-4500) and N-Benzoyl-L-tyrosine ethyl ester (BTEE, Sigma B-612.5) as substrates, respectively.
- One unit of aamylase was expressed as 1 mg glucose released per minute at 37°C. The trypsin and chymotrypsin activities were assayed using the method of Rick (1974) with N-α-Benzoyl-L-arginine ethyl-ester (BAEE, Sigma B-4500) and N-Benzoyl-L-tyrosine ethyl ester (BTEE, Sigma B-612.5) as substrates, respectively. The activities of one unit of trypsin and chymotrypsin were expressed as 1 μmole of Benzoyl-arginine and Benzoyl-tyrosine, respectively, released per min at 25°C.
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