To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of ...
To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of nucleotide sequences with the database at the NCBI. Among them, 1,230 ESTs were confirmed as novel genes, which, to date, have not been identified. Attaching the genes to a cDNA microarray slide revealed expression patterns of genes in muscle and fat according to the growth stages of swine. As specific genes expressed in the muscle tissues of swine with body weight of 30 kg, 60 genes including actin, myosin, tropomysin, transfer RNA-trp synthetase, Kel-like protein 23, KIAA0182 and COI, Foocen-m, etc were obtained. In addition, 18 novel genes were obtained. As specific genes expressed in fat tissues of swine with body weight of 30 kg, 47 genes including annexin II, Collagen, Fibronectin, Pleckstrin homology domain, serine protease, etc were obtained. 21 novel genes were also obtained. The genes specifically expressed in the muscle and fat tissues of swine affect contraction and relaxation of the muscle and the fat. However, studies on the expression mechanisms of the genes are insufficient. To reveal species of structural genes in swine muscle and fat tissue, interrelation studies in expression and function of genes by using the cDNA chip should be conducted.
To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of nucleotide sequences with the database at the NCBI. Among them, 1,230 ESTs were confirmed as novel genes, which, to date, have not been identified. Attaching the genes to a cDNA microarray slide revealed expression patterns of genes in muscle and fat according to the growth stages of swine. As specific genes expressed in the muscle tissues of swine with body weight of 30 kg, 60 genes including actin, myosin, tropomysin, transfer RNA-trp synthetase, Kel-like protein 23, KIAA0182 and COI, Foocen-m, etc were obtained. In addition, 18 novel genes were obtained. As specific genes expressed in fat tissues of swine with body weight of 30 kg, 47 genes including annexin II, Collagen, Fibronectin, Pleckstrin homology domain, serine protease, etc were obtained. 21 novel genes were also obtained. The genes specifically expressed in the muscle and fat tissues of swine affect contraction and relaxation of the muscle and the fat. However, studies on the expression mechanisms of the genes are insufficient. To reveal species of structural genes in swine muscle and fat tissue, interrelation studies in expression and function of genes by using the cDNA chip should be conducted.
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제안 방법
In this study, we generate a functional cDNA chip composed of only specific genes by searching expression profiles of genes specifically expressed in the muscle and fat tissues of Kagoshima Berkshire using cDNA microarray technology. We further attempt to apply the cDNA chip in establishing high quality seed-swine and evaluating meat quality according to varieties of swine and tissues of pork.
To discover major genes and mechanisms in the formation of muscle and fat in swine, patterns of gene expression between muscle and fat in the early growth stage of swine with body weight 30 kg were investigated using cDNA microarray (Figure 1). The genes were very well hybridized in cDNA microarray and genes specifically expressed in the muscle and fat tissues were widely distributed.
대상 데이터
The genes having information were isolated and purified by PCR. The genetic locus and map for the total 4,434 cDNAs (ESTs) were constructed. The total 4,434 cDNAs (ESTs) and 90 yeast controls were arrayed in an area of 1.
The genes having information were isolated and purified by PCR. The genetic locus and map for the total 4,434 cDNAs (ESTs) were constructed. The total 4,434 cDNAs (ESTs) and 90 yeast controls were arrayed in an area of 1.
후속연구
The benefit of finding the mechanisms is that they can be applied to the development of new seed-swine. Also, the cDNA chip prepared in this study can provide a foundation for the comparison of gene expression according to varieties and tissues of swine, gene mutation screening, genetic polymorphism interpretation, and the development of new drugs for disease treatment and disease diagnosis.
Further, it will help studies on formation of muscle in the The cDNA microarray constructed in this study makes it human body by revealing related mechanisms between easy to discover patterns of gene expression in the muscle, muscle and fat.
Obtaining related genes can help the swine-raising industry economically by controlling the growth of swine via genes that enhance muscle and fat formation in a short period. Further, it will help studies on formation of muscle in the human body by revealing related mechanisms between muscle and fat. Differences in gene expression patterns of muscle and fat in the early growth stage of swine with body weight of 30 kg were investigated using a cDNA chip of swine in order to examine genes specifically expressed in swine muscles and fat (Table 3).
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