An experiment was conducted (28 to 44 weeks) to study the laying performance, shell quality, and nutrient retention of White Leghorn layers fed different levels of non-phytate phosphorus (NPP). Six levels of NPP (0.15, 0.18, 0.21, 0.24, 0.27 and 0.30%) at a constant calcium (Ca) level (3.5%) in maize-soya-deoiled rice bran based diets were formulated, and each experimental diet was offered ad libitum for 16 weeks to five replicates with five birds in each replicate. The body weight of WL layers fed diet containing 0.15% NPP was significantly (p<0.05) lower than those fed diet with 0.30% NPP, at 44 weeks of age. However, the hen day egg production, egg weight, daily feed intake and feed consumed per dozen eggs were not influenced by the variation in the NPP levels in the diet. The bone ash content was significantly (p<0.05) higher in the birds fed 0.30% NPP as compared with those fed diets up to 0.24% NPP. Bone ash content was intermediate in the birds fed diet containing 0.27% NPP. The tibia strength followed the same trend as that of bone ash. Dietary NPP content had no influence on serum Ca and protein concentration and activity of alkaline phosphatase. However, serum inorganic P concentration increased linearly with NPP content in the diet. The concentration of P was significantly (p<0.05) higher in the birds fed 0.27% NPP or higher as compared with those fed 0.15% NPP. Levels of dietary NPP had no influence on egg quality parameters like shell wt, shell thickness, shell strength and specific gravity. The retention of nutrients such as DM, N and Ca were comparable among the WL layers fed different levels of NPP. However, the retention of P decreased linearly with increase in the level of NPP in the diet. The retention of P in the birds fed diets up to 0.24% NPP in the diet was comparable, however further increasing the content of NPP (either 0.27% or 0.30%) reduced the retention of P. Based on the results of the present study, 0.15% NPP (180 mg/b/d) in the diets of WL layers is adequate for optimum production performance during 28 to 44 weeks of age, however, WL layers require 0.27% NPP (324 mg /b/d) in the diet for optimum production with better bone mineralization.
AOAC. 1990. Official Methods of Analysis of the Association of Official Analytical Chemists (Virginia, USA, Association of Official Analytical Chemists).
Boling, S. D., M. W. Douglas, M. L. Johnson, X. Wang, C. M. Parsons, K. W. Koelkebeck and R. A. Zimmerman. 2000. The effects of dietary available phosphorus levels and phytase on performance of young and older laying hens. Poult. Sci. 79:224-230.
Garlich, J. D., R. L. James and J. B. Ward. 1985. Effect of short terms phosphorus deprivation of laying hens. Poult. Sci. 64:1193-1199.
Gordon, R. W. and D. A. Roland, Sr. 1997. Performance of commercial laying hens fed various phosphorus levels with and without supplemental phytase. Poult. Sci. 76:1172-1177.
Hartel, H. 1990. Evaluation of the dietary interaction of calcium, and phosphorus in the high producing laying hen. Br. Poult. Sci. 31:473-494.
Keshavarz, K. 2003. The effect of different levels of nonphytate phosphorus with and without phytase on the performance of four strains of laying hens. Poult. Sci. 82:71-91.
National Research Council. 1994. Nutrient requirements of poultry 9th rev. ed. National Academy Press, Washington, DC.
Parsons, C. M. 1999. The effect of dietary available phosphorus and phytase level on long-term performance of laying hens. Pages 24-33 in: BASF Technical Symposium, Use of Natuphos Phytase in Layer Nutrition and Management, Atlanta, GA.
Pointillart, A., A. Fourdin and N. Fontaine. 1987. Importance of cereal phytase activity for phytate phosphorus utilization by growing pigs fed diets containing triticale corn. J. Nutr. 117:907-913.
Rama Rao, S. V., R. V. Reddy and V. R. Reddy. 1999a. Enhancement of phytate phosphorus availability in the diets of commercial broilers and layers. Anim. Feed Sci. Tech. 79:211-222.
Snedecor, G. W. and W. G. Cochran. 1989. Statistical Methods. Oxford and IBH Publishing Company, New Delhi.
Sohail, S. S. and D. A. Roland, Sr. 2002. Influence of dietary phosphorus on performance of Hy-Line W36 hens. Poult. Sci. 81:75-83.
Summers, J. D. 1995. Reduced dietary phosphorus levels for layers. Poult. Sci. 74:1977-1983.
Van der Klis, J. D., H. A. J. Versteegh and P. C. M. Simons. 1996. Natuphos in laying hen nutrition. Pages 71-82 in: BASF Technical Symposium, Phosphorus and Calcium Management in Layers, Atlanta, GA. BASF Corporation, Mt. Olive, NJ.
Keshavarz, K. 2000. Nonphytate phosphorus requirement of laying hens with and without phytase on a phase feeding program. Poult. Sci. 79:748-763.
Owings, W. J., L. Sell and S. L. Balloun. 1977. Dietary phosphorus needs of laying hens. Poult. Sci. 56:2056-2060.
Parsons, C. M. 1999.The effect of dietary available phosphorus and phytase level on long term performance of laying hens. Pages 24-33 in: BASF Technical Symposium, Atlanta, GA.
Rama Rao, S. V., V. R. Reddy and R. V. Reddy. 1999b. Non-phytin phosphorus requirements of commercial broilers and White leghorn layers. Anim. Feed Sci. Tech. 80:1-10.
Doumas, B. T., W. A. Watson and H. G. Biggs. 1971. Albumin standard and the measurement of serum albumin with bromocresol green. Clinical Chem. Acta 31:87.
Duncan, D. B. 1955. Biometrics, 11:1-42.
Roland, D. A., Sr. and R. W. Gordon. 1996. Phosphorus and calcium optimization in layer diets with phytase. Pages 305-316 in: BASF Technical Symposium, Phytase in Animal Nutrition and Waste Management, Atlanta, GA. BASF Corporation, Mt. Olive, NJ.
Paik, I. 2003. Application of phytase, microbial or plant origin, to reduce phosphorus excretion in poultry production. Asian-Aust. J. Anim. Sci. 16:124-135.
Keshavarz, K. 1996. The effect of different levels of vitamin C, and cholecalciferol with adequate or marginal levels of dietary calcium on performance, and egg shell quality of laying hens. Poult. Sci. 75:1227-1235.
Selle, P. H., V. Ravindran, P. H. Pittolo and W. L. Bryden. 2003. Effects of phytase supplementation of diets with two specifications on growth performance and protein efficiency ratios of broiler chickens. Asian-Aust. J. Anim. Sci. 16:1158-1164.
Bergmeyer, H. U. 1974. In Methods of Enzymatic Analysis. Vol. 11.Academic Press. Inc; USA.
Miles, R. D., P. T. Costa and R. H. Harms. 1983. The influence of dietary phosphorus levels on laying hen performance, egg shell quality and various blood parameters. Poult. Sci. 62:1033-1037.
Sell, J. L. 1979. Phosphorus requirement of laying hens: A basic approach, Feed Manage. 30:24-29.
Scott, M. L., M. C. Neshim and R. J. Young. 1982. Nutrition of the chicken. ML Scott and Associates,Ithaca, NY.
Usayran, N. and D. Balnave. 1995. Phosphorus requirements of laying hens fed on wheat based diets. Br. Poult. Sci. 36:285-301.
Sell, J. L., S. E. Scheideler and B. E. Rahn. 1987. Influence of different phosphorus phase feeding programmes and dietary calcium level on performance and body phosphorus of laying hens. Poult. Sci. 6:1524-1530.
Fiske, C. H. and Y. Subba Row. 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66:375-400.
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2007. "" Asian-Australasian journal of animal sciences, 20(6): 933~938