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Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene

Asian-Australasian journal of animal sciences, v.18 no.1, 2005년, pp.3 - 7  

Kim, J.Y. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ,  Yoon, D.H. (National Livestock Research Institute, RDA) ,  Park, B.L. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ,  Kim, L.H. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ,  Na, K.J. (National Livestock Research Institute, RDA) ,  Choi, J.G. (National Livestock Research Institute, RDA) ,  Cho, C.Y. (National Livestock Research Institute, RDA) ,  Lee, H.K. (Department of Biotechnology, Hankyong National University) ,  Chung, E.R. (Department of Biotechnology, Sangi University) ,  Sang, B.C. (Department of Dairy Science, Graduate School, Chungnam National University) ,  Cheong, I.J. (National Livestock Research Institute, RDA) ,  Oh, S.J. (National Livestock Research Institute, RDA) ,  Shin, Hyoung Doo (Department of Genetic Epidemiology, SNP Genetics, Inc.)

Abstract AI-Helper 아이콘AI-Helper

The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivit...

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제안 방법

  • Among identified SNPs, five SNPs; IGFBP3 G-854C in promoter, IGFBP3 G-100A in 5’UTR, IGFBP3 G+421T, IGFBP3 T+1636A in intron 1 and IGFBP3 C+3863A in intron 2 were selected for large scale screening on the base of the location and frequency.

대상 데이터

  • Among identified SNPs, five SNPs; IGFBP3 G-854C in promoter, IGFBP3 G-100A in 5’UTR, IGFBP3 G+421T, IGFBP3 T+1636A in intron 1 and IGFBP3 C+3863A in intron 2 were selected for large scale screening on the base of the location and frequency. And then these five common SNPs were screened in 650 Korean cattle (National Livestock Research Institute, Korea).
  • Among identified SNPs, five SNPs; IGFBP3 G-854C in promoter, IGFBP3 G-100A in 5’UTR, IGFBP3 G+421T, IGFBP3 T+1636A in intron 1 and IGFBP3 C+3863A in intron 2 were selected for large scale screening on the base of the location and frequency. And then these five common SNPs were screened in 650 Korean cattle (National Livestock Research Institute, Korea). The frequencies of those SNPs were 0.
  • DNA samples for genotyping were isolated from 650 Korean cattle in National Livestock Research Institute, Korea. Primer extension reactions were performed with SNaPSHOT ddNTP Primer Extension Kit (Applied Biosystems, Foster City, CA).
  • Full gene including -1,500 bp promoter region were PCR-amplified and directly sequenced using ABI PRISM 3700 genetic analyzer (Applied Biosystems, Foster City CA). Seventeen primer sets for the amplification and sequencing analysis were designed based on GenBank sequences (AF_305712) (Table 1). Sequence variants were verified by chromatograms.
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참고문헌 (19)

  1. Baxter, R. C. 1993. IGF binding protein-3 and the acid-labile subunit: formation of the ternary complex in vitro and in vivo. Adv. Exp. Med. Biol. 343:237-244. 

  2. Clemmons, D. R. 1993. IGF binding proteins and their functions. Mol. Reprod. Dev. 35(4):368-74; discussion 374-375. 

  3. Clemmons, D. R. 1997. Insulin-like growth factor binding proteins and their role in controlling IGF actions. Cytokine Growth Factor Rev. 8(1):45-62. 

  4. Collett-Solberg, P. F. and P. Cohen. 1996. The role of the insulinlike growth factor binding proteins and the IGFBP proteases in modulating IGF action. Endocrinol. Metab. Clin. North Am. 25(3):591-614. 

  5. D'Ercole, A. J. 1987. Somatomedins/insulin-like growth factors and fetal growth. J. Dev. Physiol. 9(6):481-495. 

  6. Ferry, R. J., Jr., R. W. Cerri and P. Cohen. 1999. Insulin-like growth factor binding proteins: new proteins, new functions. Horm. Res. 51(2):53-67. 

  7. Ferry, R. J., Jr., L. E. Katz, A. Grimberg, P. Cohen and S. A. Weinzimer. 1999. Cellular actions of insulin-like growth factor binding proteins. Horm. Metab. Res. 31(2-3):192-202. 

  8. Hedrick, P. W. 1987. Gametic disequilibrium measures: proceed with caution. Genetics 117(2):331-341. 

  9. Jones, J. I. and D. R. Clemmons. 1995. Insulin-like growth factors and their binding proteins: biological actions. Endocr. Rev. 16(1):3-34. 

  10. Kappes, S. M., J. W. Keele, R. T. Stone, R. A. McGraw, T. S. Sonstegard, T. P. Smith, N. L. Lopez-Corrales and C. W. Beattie. 1997. A second-generation linkage map of the bovine genome. Genome. Res. 7(3):235-249. 

  11. Liu, F., D. R. Powell and R. L. Hintz. 1990. Characterization of insulin-like growth factor-binding proteins in human serum from patients with chronic renal failure. J. Clin. Endocrinol. Metab. 70(3):620-628. 

  12. Maciulla, J. H., H. M. Zhang and S. K. DeNise. 1997. A novel polymorphism in the bovine insulin-like growth factor binding protein-3 (IGFBP3) gene. Anim. Genet. 28(5):375. 

  13. Oh, Y., H. L. Muller, G. Lamson and R. G. Rosenfeld. 1993. Insulin-like growth factor (IGF)-independent action of IGFbinding protein-3 in Hs578T human breast cancer cells. Cell surface binding and growth inhibition. J. Biol. Chem. 268(20):14964-14971. 

  14. Phillips, L. S., C. I. Pao and B. C. Villafuerte. 1998. Molecular regulation of insulin-like growth factor-I and its principal binding protein, IGFBP3. Prog. Nucleic Acid Res. Mol. Biol. 60:195-265. 

  15. Ryu, Y., J. Yang, D. Seo, and Y. Ko. 2003. Effect of Serum IGF-I on Progesterone Concentration during Early Pregnancy in Korean Native Cattle (Hanwoo). Asian-Aust. J. Anim. Sci. 16(1):176-179. 

  16. Stewart, C. E. and P. Rotwein. 1996. Growth, differentiation, and survival: multiple physiological functions for insulin-like growth factors. Physiol. Rev. 76(4):1005-1026. 

  17. Thissen, J. P., J. M. Ketelslegers and L. E. Underwood. 1994. Nutritional regulation of the insulin-like growth factors. Endocr. Rev. 15(1):80-101. 

  18. Thue, T. D. and F. C. Buchanan. 2002. A new polymorphism in the cattle IGFBP3 gene. Anim. Genet. 33(3):242. 

  19. Valentinis, B., A. Bhala, T. DeAngelis, R. Baserga and P. Cohen. 1995. The human insulin-like growth factor (IGF) binding protein-3 inhibits the growth of fibroblasts with a targeted disruption of the IGF-I receptor gene. Mol. Endocrinol. 9(3):361-367. 

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