Olowofeso, O.
(Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
,
Wang, J.Y.
(Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
,
Shen, J.C.
(Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
,
Chen, K.W.
(Institute of Poultry Science)
,
Sheng, H.W.
(Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
,
Zhang, P.
(Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
,
Wu, R.
(Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polyme...
To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polymerase chain reaction (PCR) with ten microsatellite markers. Amplified PCR-products with the selected markers were separated on a 12% polyacrylamide gel with pBR322DNA/MspI used as internal standard marker. Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus, effective number of alleles ($N_e$) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. The mean allele number for all loci ranged between 4.00${\pm}$0.33 (Rugao) to 4.90${\pm}$0.48 (Cshiqishi) and across populations for all loci was 4.60${\pm}$0.20, while (H) ranged from 0.65${\pm}$0.03 (Rugao) to 0.69${\pm}$0.03 (Jiangchun) among loci and across populations, (H) was 0.67${\pm}$0.01. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the four Haimen chicken populations was 98.75%.
To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polymerase chain reaction (PCR) with ten microsatellite markers. Amplified PCR-products with the selected markers were separated on a 12% polyacrylamide gel with pBR322DNA/MspI used as internal standard marker. Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus, effective number of alleles ($N_e$) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. The mean allele number for all loci ranged between 4.00${\pm}$0.33 (Rugao) to 4.90${\pm}$0.48 (Cshiqishi) and across populations for all loci was 4.60${\pm}$0.20, while (H) ranged from 0.65${\pm}$0.03 (Rugao) to 0.69${\pm}$0.03 (Jiangchun) among loci and across populations, (H) was 0.67${\pm}$0.01. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the four Haimen chicken populations was 98.75%.
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문제 정의
Several authors have reported the beneficial values of microsatellite markers in several population studies. The this work was to appraise further the additional usefulness of microsatellite markers with chickens in China whose genetic information have not been included in the common chicken populations. Based on the results of this investigation, the generated mean allele number across populations ranged from 2.
제안 방법
The allele frequencies (data unpublished) obtained by the microsatellite markers were used to determine the within locus (hi) heterozygosity, effective number of alleles (Ne) and polymorphism information content (PIC) among the respective populations. The mean allele number among loci ranged from 4.
In the current investigation however, we have amplified chicken genomic DNAs, determine the genetic diversity indices in the chicken populations under consideration and integrate the unbiased average heterozygosity value in each population into the global formula of cumulative power of discrimination (CPD) to generate this important parameter. The objective of this study was therefore to estimate the CPD in Haimen chicken populations using ten microsatellite markers.
대상 데이터
The sample collection was carried out in Haimen Integrated Poultry Company and analyses were carried out at the research laboratory of the Genetics and Breeding Unit, Yangzhou University, Jiangsu Province, East China, between April, 2003 to June, 2004. The sample size by population were Rugao yellow chicken (RYC) 62, Jiangchun yellow chicken (JYC) 62, Wan-Nan yellow chicken (WYC) 63 and Cshiqishi yellow chicken (CYC) 65, respectively.
이론/모형
(1988). Concentration of the DNA solution was measured based on the micro-gel method (Sambrook et al., 1989) and each DNA was adjusted to 100 ng/μl. Genotyping of DNA samples at the ten selected microsatellite markers were carried out using the isolated DNA from chickens of different populations.
(1988). Concentration of the DNA solution was measured based on the micro-gel method (Sambrook et al., 1989) and each DNA was adjusted to 100 ng/μl. Genotyping of DNA samples at the ten selected microsatellite markers were carried out using the isolated DNA from chickens of different populations.
성능/효과
The this work was to appraise further the additional usefulness of microsatellite markers with chickens in China whose genetic information have not been included in the common chicken populations. Based on the results of this investigation, the generated mean allele number across populations ranged from 2.50 (MCW0294) to 6.25 (MCW0183) indicating that the microsatellite markers used were variable and polymorphic with Haimen chicken populations. The observed mean allele number among loci in each of the chicken population as well as the unbiased average heterozygosities in this work were consistent with what was reported by Barker (1994) and Wimmers et al.
The this work was to appraise further the additional usefulness of microsatellite markers with chickens in China whose genetic information have not been included in the common chicken populations. Based on the results of this investigation, the generated mean allele number across populations ranged from 2.50 (MCW0294) to 6.25 (MCW0183) indicating that the microsatellite markers used were variable and polymorphic with Haimen chicken populations. The observed mean allele number among loci in each of the chicken population as well as the unbiased average heterozygosities in this work were consistent with what was reported by Barker (1994) and Wimmers et al.
, 2002). In this work, we observed a CPD of 98.75% showing clearly that there is high genetic variations among the Haimen chicken populations tested.
, 2002). In this work, we observed a CPD of 98.75% showing clearly that there is high genetic variations among the Haimen chicken populations tested.
The results of this experiment revealed that microsatellite markers is/are sufficient enough as molecular marker to estimate all the genetic information in species populations. This survey apart from showing the within and among chicken population genetic information, it revealed the cumulative power of discrimination among the four Haimen chicken populations in China to be exactly 98.
The results of this experiment revealed that microsatellite markers is/are sufficient enough as molecular marker to estimate all the genetic information in species populations. This survey apart from showing the within and among chicken population genetic information, it revealed the cumulative power of discrimination among the four Haimen chicken populations in China to be exactly 98.75%.
참고문헌 (19)
Barker, J. S. F. 1994. A global protocol for determining genetic distances among domestic livestock breeds. In: Proceedings of the 5th World Congress of Genetics Applied to Livestock Production. 21:501-508.
Bartfai, R., S. Egedi, G. H. Yue, B. Kovacs, B. Urbanyi, G. Tamas, L. Horvath and L. Orban. 2003. Genetic analysis of two common carp broodstocks by random amplified polymorphic DNA (RAPD) and microsatellite markers. J. Aquaculture 219:157-167.
Botstein, D., R. L. White and M. Skolnick. 1980. Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331.
Chen, G. H., X. S. Wu, D. Q. Wang, J. Qin, S. L. Wu, Q. L. Zhou, F. Xie, R. Cheng, Q. Xu, B. Liu, X. Y. Zhang and O. Olowofeso. 2004. Cluster analysis of 12 Chinese native chicken populations using microsatellite markers. Asian-Aust. J. Anim. Sci. 17(8):1047-1052.
Chen, H., L. Leibenguth and Z. Bi-cai. 2002. DNA fingerprinting in five animal species using microsatellite probes. In: Proceedings of the 8th National Symposium on Animal Genetic Markers. 8(1):67-71, Oct., Yangling, China.
Chenyambuga, S. W., O. Hanotte, J. Hirbo, P. C. Watts, S. J. Kemp, G. C. Kifaro, P. S. Gwakisa, P. H. Petersen and J. E. O. Rege. 2004. Genetic characterization of indigenous Goats of Subsaharan Africa using microsatellite DNA markers. Asian-Aust. J. Anim. Sci. 17(4):445-452.
Emara, M. G. and H. Kim. 2003. Genetic markers and their application in Poultry Breeding. Poult. Sci. 82:952-957.
Fan, B., B. Liu and K. Li. 2002. The application of microsatellite DNA marker in animal individual verification and breed assignment. In: Proceedings of the 8th National Symposium on Animal Genetic Markers. 8(1):35-38, Oct., Yangling, China.
Miller, S. A., D. D. Dykes and H. F. Plosky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:1215.
Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.
Ponsuksili, S., K. Wimmers, F. Scmoll, P. Horst and K. Schellander. 1999. Comparison of multilocus DNA fingerprints and microsatellites in an estimate of genetic distances in chicken. J. Hered. 6:656-659.
Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular cloning- A laboratory manual. Vol. 2, 2nd edition. Cold Spring Harbour, Laboratory Press, USA.
Sano, A., Z. Huiling, M. Kimura, C. Hong and K. Nosawa. 1997. Genetic variability in commercial Quail populations in Shaanxi, China. In: Studies on Animal Genetic Resources in China, Edited by Chang Hong, Nov., Pages 223-229.
Shen, J. C. 2004. Study on genetic diversity of nine indigenous Chinese chicken breeds using microsatellite markers. M.Sc. Thesis, Yangzhou University, Yangzhou, China.
Vanhala, T., M. Tuiskala-Haavisto, K. Elo, J. Vilkki and A. Maki-Tanila. 1998. Evaluation of genetic variability and genetic distances between eight chicken lines using microsatellite markers. Poult. Sci. 77:783-790.
Wimmers, K., S. Ponsuksili, T. Hardge, A. Valle-Zarate, P. K. Mathur and P. Horst. 2000. Genetic distinctness of African, Asian and South American local chickens. Anim. Genet. 31:159-165.
Wu, X. S. 2004. Study on genetic diversity in Chinese indigenous chicken breeds using microsatellite markers and their relationships between performance and microsatellite loci. Ph.D. Thesis, Yangzhou University, Yangzhou, China.
Yang, Z. P., H. Chang, W. Sun, R. Q. Gen, Y. J. Mao and K. Tsunoda. 2004. A comparison of two kinds of markers applied in analysis of genetic diversity in Sheep and Goat populations. Asian-Aust. J. Anim. Sci. 17(7):892-896.
Zhang, X., F. C. Leung, D. K. O. Chan, G. Yang and C. Wu. 2002. Genetic diversity of Chinese native chicken breeds based on protein polymorphism, random amplified polymorphic DNA and microsatellite polymorphism. Poult. Sci. 81:1463-1472.
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