This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of guineagrass silage were also investigated. The pH value of the silages treated with FJLB rapidly decreased, and reached to the lowest value within 7 days of start of fermentation, as compared to the control. The number of lactic acid bacteria (LAB) in the treated silages increased for the first 3 days, thereafter the number of LAB declined gradually up to the end of the experiment. Silages treated with FJLB had larger populations of LAB than the control. Ammonia-nitrogen production increased throughout the ensiling period, which in the control and no-sugar added FJLB silages were higher than the other treated silages. Lactic acid levels varied with the time of ensiling and among the silage treatments. For any sugar FJLB treated silages, the lactic acid increased initially, and then slightly reduced to less than 50 g/kg of dry matter until 49 days after ensiling, except the silage treated with glucose added FJLB. Nevertheless, lactic acid content of the control decreased constantly from the beginning of ensiling and was not found after 35 days. Moreover, acetic acid content increased throughout the ensiling period. All the FJLB treated silages had significantly (p<0.05) lower pH and ammonia-nitrogen content, while significantly (p<0.05) higher lactic acid content and V-score value compared with the control. This study confirmed that the applying of FJLB with any sugar substrate improved fermentative quality of silage.
Kozaki, M., T. Uchimura and S. Okada. 1992. Experimental Manual of Lactic acid bacteria. Asakurashoten, Tokyo, Japan (Jpn). pp. 6-16.
Muck, R. E. 1990. Prediction of lactic acid bacterial numbers on Lucerne. Grass and Forage Sci. 45:273-280.
Murphy, R. P. 1958. A method for the extraction of plant sample and the determination of total soluble carbohydrates. J. Sci. Food Agric. 9:714-717.
Shao, T., N. Ohba, M. Shimojo and Y. Masuda. 2004. Effects of adding glucose, sorbic acid and pre-fermented juice on the fermentation quality of guineagrass (Panicum maximum Jacq.) silage. Asian-Aust. J. Anim. Sci. 17:808-813.
Shockey, W. L., B. A. Dehority and H. R. Conrad. 1988. Effects of microbial inoculant on fermentation of poor quality alfalfa. J. Dairy Sci. 71:722-726.
Ohmomo, S., O. Tanaka, H. K. Kitamoto and Y. Cai. 2002. Silage and microbial performance, Old story but new problems. Jpn. Agric. Res. Quart. 36:59-71.
Yahaya, M. S., M. Goto, W. Yimiti, B. Smerjai and Y. Kawamoto. 2004. Evaluation of fermentation quality of a tropical and temperature forage crops ensiled with additives of fermented juice of epiphytic lactic acid bacteria (FJLB). Asian-Aust. J. Anim. Sci. 17:942-946.
Hellings Ph., G. Bertin and M. Vambelle. 1985. Effect of lactic acid bacteria on silage fermentation. Proceedings of 15th International Grassland Congress, August 24-31, Kyoto, Japan. pp. 932-933.
Japan Grassland Farming Forage Seed Association. 1994. Guide Book for Quality Evaluation of forage. Tokyo, Japan (Jpn). pp. 82-87.
Playne, M. J. and P. McDonald. 1966. The buffering constituents of herbage and of silage. J. Sci. Food Agric. 17:264-268.
Niimi, M. and O. Kawamura. 1998. Degradation of cell wall constituents of Guineagrass (Panicum maximum Jacq.) during ensiling. J. Jpn. Grassl. Sci. (Jpn) 43:413-417.
Imura, Y., T. Namihira and Y. Kawamoto. 2001. Fermentation quality of phasey bean and guineagrass silages. In Proceedings of 19th Int.Grass. Congr.,Soa Pedro, Sao Paulo, Brazil, pp. 784-785.
SAS. 1998. The SAS systems for windows v6.12. SAS Inst. Inc., Cary, N.C., USA.
Lin, C., K. K. Bolsen, B. E Brent, R. A. Hart, A. M. Feyerherm and W. R. Aimutis. 1992. Epiphytic microflora on alfalfa and whole-plant corn. J. Dairy Sci. 75:2484-2493.
Woolford, M. K. and M. K. Sawczyc. 1984. An investigation into the effect of cultures of lactic acid bacteria on fermentation in silage 1. Strain selection. Grass and Forage Sci. 39:139-184.
Cai, Y., Y. Benno, M. Ogawa, S. Ohmomo, S. Kumai and T. Nakase. 1998. Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Appl. Envi. Microb. 64:2982-2987.
Lindgren, S. E., L.T. Axelsson and R. F. Mcfeeters. 1990. Anaerobic L-lactate degradation by Lactobacillus Plantarum. FEMS Microbiol. Lett. 66:209-214.
Ohshima, M., E. Kimura and H. Yokota. 1996. A method of making good quality silage from direct cut alfalfa by spraying previously fermented juice. Anim. Feed Sci. Technol. 66:129-137.
이 논문을 인용한 문헌 (4)
2007. "" Asian-Australasian journal of animal sciences, 20(6): 920~924
2007. "" Asian-Australasian journal of animal sciences, 20(4): 523~528
2007. "" Asian-Australasian journal of animal sciences, 20(6): 887~893
2011. "" Asian-Australasian journal of animal sciences, 24(3): 358~363