Bureenok, S.
(Faculty of Agriculture, University of the Ryukyus)
,
Namihira, T.
(Faculty of Agriculture, University of the Ryukyus)
,
Tamaki, M.
(Faculty of Agriculture, University of the Ryukyus)
,
Mizumachi, S.
(Faculty of Agriculture, University of the Ryukyus)
,
Kawamoto, Y.
(Faculty of Agriculture, University of the Ryukyus)
,
Nakada, T.
(Faculty of Agriculture, University of the Ryukyus)
This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of gu...
This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of guineagrass silage were also investigated. The pH value of the silages treated with FJLB rapidly decreased, and reached to the lowest value within 7 days of start of fermentation, as compared to the control. The number of lactic acid bacteria (LAB) in the treated silages increased for the first 3 days, thereafter the number of LAB declined gradually up to the end of the experiment. Silages treated with FJLB had larger populations of LAB than the control. Ammonia-nitrogen production increased throughout the ensiling period, which in the control and no-sugar added FJLB silages were higher than the other treated silages. Lactic acid levels varied with the time of ensiling and among the silage treatments. For any sugar FJLB treated silages, the lactic acid increased initially, and then slightly reduced to less than 50 g/kg of dry matter until 49 days after ensiling, except the silage treated with glucose added FJLB. Nevertheless, lactic acid content of the control decreased constantly from the beginning of ensiling and was not found after 35 days. Moreover, acetic acid content increased throughout the ensiling period. All the FJLB treated silages had significantly (p<0.05) lower pH and ammonia-nitrogen content, while significantly (p<0.05) higher lactic acid content and V-score value compared with the control. This study confirmed that the applying of FJLB with any sugar substrate improved fermentative quality of silage.
This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of guineagrass silage were also investigated. The pH value of the silages treated with FJLB rapidly decreased, and reached to the lowest value within 7 days of start of fermentation, as compared to the control. The number of lactic acid bacteria (LAB) in the treated silages increased for the first 3 days, thereafter the number of LAB declined gradually up to the end of the experiment. Silages treated with FJLB had larger populations of LAB than the control. Ammonia-nitrogen production increased throughout the ensiling period, which in the control and no-sugar added FJLB silages were higher than the other treated silages. Lactic acid levels varied with the time of ensiling and among the silage treatments. For any sugar FJLB treated silages, the lactic acid increased initially, and then slightly reduced to less than 50 g/kg of dry matter until 49 days after ensiling, except the silage treated with glucose added FJLB. Nevertheless, lactic acid content of the control decreased constantly from the beginning of ensiling and was not found after 35 days. Moreover, acetic acid content increased throughout the ensiling period. All the FJLB treated silages had significantly (p<0.05) lower pH and ammonia-nitrogen content, while significantly (p<0.05) higher lactic acid content and V-score value compared with the control. This study confirmed that the applying of FJLB with any sugar substrate improved fermentative quality of silage.
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제안 방법
Ltd, Vacuum sealer, SQ202). The silos were kept at 25℃ and samples were taken 1, 3, 7, 14, 21, 35 and 49 days after ensiling for chemical analysis.
However, very few researches reported about ensiling of tropical pasture species treated with FJLB. This study is purposed to clarify if the applying FJLB with addition of glucose, sucrose, and molasses as a substrate would enhance the fermentation of the guineagrass silage.
데이터처리
Data obtained on chemical composition of silage ensiled for 49 days were analyzed using the GLM procedure of SAS and the significance of the difference among treatment means was determined by the Duncan’s multiple-range test (SAS, 1998).
Data obtained on chemical composition of silage ensiled for 49 days were analyzed using the GLM procedure of SAS and the significance of the difference among treatment means was determined by the Duncan’s multiple-range test (SAS, 1998).
이론/모형
Dry matter content of silage was determined by oven drying at 70℃ to a constant weight. The content of water soluble carbohydrate (WSC) was estimated colorimetrically using Anthrone method (Murphy, 1958). Buffering capacity of material grass was determined according to the method as described by Playne and McDonald (1966).
성능/효과
, 2004). In this study, the applying of any sugar treated FJLB as a silage additive showed improved the fermentative quality of silage with a high concentration of lactic acid and a low concentration of NH3-N.
This experiment also showed significantly different results with the variety of sugar added in FJLB. It was found that the glucose-added FJLB was able to decrease the pH value more rapidly than the others, and fortunately, lactic acid content constantly remained during the ensiling period. Although, these FJLB were made from the same crop and the LAB counts were similar before the used of silage additives, the difference of the fermentative quality was may be due to the change of LAB species and their percentages in each substrate during incubation and after ensiling.
Moreover, the LAB number of these silages was lower than the treated silages in any ensiling time. The study clearly revealed that all sugar FJLB silages at the earlier ensiling period were able to decrease pH more rapidly and increase the LAB number dramatically in the same time.
Although, these FJLB were made from the same crop and the LAB counts were similar before the used of silage additives, the difference of the fermentative quality was may be due to the change of LAB species and their percentages in each substrate during incubation and after ensiling. This study suggests that the FJLB treated with glucose probably consisted of effective LAB species with the highest percentage than that in other sugar substrate.
후속연구
The results obviously confirmed that using of any sugar treated FJLB additive somehow would be one of the ways to improve the fermentative quality of silage in the tropical area. Though, the FJLB also contain many kinds of microorganism and a variety of strains as mentioned, further researches must be needed to identify the actual useful species of bacteria, and to clarify the mechanism of the bacteria for improving quality of silage.
참고문헌 (18)
Cai, Y., Y. Benno, M. Ogawa, S. Ohmomo, S. Kumai and T. Nakase. 1998. Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Appl. Envi. Microb. 64:2982-2987.
Hellings Ph., G. Bertin and M. Vambelle. 1985. Effect of lactic acid bacteria on silage fermentation. Proceedings of 15th International Grassland Congress, August 24-31, Kyoto, Japan. pp. 932-933.
Imura, Y., T. Namihira and Y. Kawamoto. 2001. Fermentation quality of phasey bean and guineagrass silages. In Proceedings of 19th Int.Grass. Congr.,Soa Pedro, Sao Paulo, Brazil, pp. 784-785.
Japan Grassland Farming Forage Seed Association. 1994. Guide Book for Quality Evaluation of forage. Tokyo, Japan (Jpn). pp. 82-87.
Kozaki, M., T. Uchimura and S. Okada. 1992. Experimental Manual of Lactic acid bacteria. Asakurashoten, Tokyo, Japan (Jpn). pp. 6-16.
Lin, C., K. K. Bolsen, B. E Brent, R. A. Hart, A. M. Feyerherm and W. R. Aimutis. 1992. Epiphytic microflora on alfalfa and whole-plant corn. J. Dairy Sci. 75:2484-2493.
Lindgren, S. E., L.T. Axelsson and R. F. Mcfeeters. 1990. Anaerobic L-lactate degradation by Lactobacillus Plantarum. FEMS Microbiol. Lett. 66:209-214.
Muck, R. E. 1990. Prediction of lactic acid bacterial numbers on Lucerne. Grass and Forage Sci. 45:273-280.
Niimi, M. and O. Kawamura. 1998. Degradation of cell wall constituents of Guineagrass (Panicum maximum Jacq.) during ensiling. J. Jpn. Grassl. Sci. (Jpn) 43:413-417.
Ohmomo, S., O. Tanaka, H. K. Kitamoto and Y. Cai. 2002. Silage and microbial performance, Old story but new problems. Jpn. Agric. Res. Quart. 36:59-71.
Ohshima, M., E. Kimura and H. Yokota. 1996. A method of making good quality silage from direct cut alfalfa by spraying previously fermented juice. Anim. Feed Sci. Technol. 66:129-137.
Playne, M. J. and P. McDonald. 1966. The buffering constituents of herbage and of silage. J. Sci. Food Agric. 17:264-268.
SAS. 1998. The SAS systems for windows v6.12. SAS Inst. Inc., Cary, N.C., USA.
Shao, T., N. Ohba, M. Shimojo and Y. Masuda. 2004. Effects of adding glucose, sorbic acid and pre-fermented juice on the fermentation quality of guineagrass (Panicum maximum Jacq.) silage. Asian-Aust. J. Anim. Sci. 17:808-813.
Shockey, W. L., B. A. Dehority and H. R. Conrad. 1988. Effects of microbial inoculant on fermentation of poor quality alfalfa. J. Dairy Sci. 71:722-726.
Woolford, M. K. and M. K. Sawczyc. 1984. An investigation into the effect of cultures of lactic acid bacteria on fermentation in silage 1. Strain selection. Grass and Forage Sci. 39:139-184.
Yahaya, M. S., M. Goto, W. Yimiti, B. Smerjai and Y. Kawamoto. 2004. Evaluation of fermentation quality of a tropical and temperature forage crops ensiled with additives of fermented juice of epiphytic lactic acid bacteria (FJLB). Asian-Aust. J. Anim. Sci. 17:942-946.
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