[논문]Effect of Different Level of Monensin Supplemented with Cold Process Urea Molasses Mineral Block on In vitro Rumen Fermentation at Different Days of Adaptation with Monensin

De, Debasis; Singh, G.P.

doi:10.5713/ajas.2005.320

Effect of Different Level of Monensin Supplemented with Cold Process Urea Molasses Mineral Block on In vitro Rumen Fermentation at Different Days of Adaptation with Monensin 원문보기

Asian-Australasian journal of animal sciences, v.18 no.3, 2005년, pp.320 - 325

De, Debasis (lCAR Research Complex for NEH Region, Sikkim Centre) , Singh, G.P. (Division of Dairy Cattle Nutrition, National Dairy Research Institute)

Abstract ▼ AI-Helper

Effect of period of adaptation and levels of monensin were studied for microbial fermentation/ digestibility to find out the optimum period of adaptation of monensin in rumen and suitable level of monensin in wheat straw+concentrate and wheat straw+UMMB diet. The mean digestibility of dry matter was decreased upto T-3 treatment (49.17%), however, digestibility of DM was affected upto period (P-2). NDF digestibility was affected due to treatment under P1 and P2 (p<0.05). Average digestibility of ADF was increased to 53.33% at T-3 level of monensin and P4 days of adaptation. TVFA (mmole/100 ml) were decreased from 9.49 in T-1 to 7.70 in T-7. Periods were not effectives except P2 (14 days of adaptation). Similarly, total gas was decrease with the increase of monensin levels in diet. Although acetate percentage in TVFA was not affected either due to level of monensin or period of adaptation but propionate was increased due to increase in monensin at 21 days of adaptation (P-3). Butyrate (%) was decreased significantly in T-2 to T-6 as compared to T-1 group. Total gas was significantly (p<0.01) higher in group T-1 (control) and it reduced significantly in T-5, however, differences in gas production between group T-3, T-5 and T-7 at P-1 was not significant. Methane production was reduced on P-3 and P-4 level of adaptation due to treatment. The overall result indicated that 21 days of adaptation with monensin was sufficient to mask the inhibiting effect of monensin to cell wall digestibility and 35 ppm monensin is optimum to reduce methane production and increase propionate productions.

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제안 방법

Dry matter, cell wall digestibility and volatile fatty acid: 1n vitro DM, NDF and ADF digestibility was determined in the sample by measuring the difference of DM (AOAC, 1984), NDF and ADF content (Vansoest et al., 1991) before and after in vitro digestion of sample. Rumen fluid was analyzed for TVFA concentration (Bernett and Reid, 1957) and molar proportion of individual VFA (Erwin et al.
, 1979). Measurement of total gas production was done at 4, 8, 12, 18, 24, 30, 36, 42 and 48 h of incubation by observing the displacement of plunger of syringe. Proportion of methane in total gas was measured after 48 h of incubation using gas chromatography.

성능/효과

In P Ⅱ, when donor animals were adapted to monensin for 14 d, DM digestibility of monensin (up to 100 ppm level) enriched UMMB treatments was at per with UMMB without monensin treatment (T2), and beyond 100 ppm level DM digestibility decreased.
NDF digestibility was higher (p0.05) in ADF digestibility was observed among treatments, In P Ⅲ and P IV, similar DM and cell wall digestibility were observed except for ADF digestibility in P IV, where ADF digestibility was higher (p<0.01) in monensin enriched UMMB treatments as compared to that of treatments without monensin supplementation, Similar DM and cell wall digestibility in all treatments in P Ⅲ and P IV might be due to the fact that after 14 d of monensin feeding rumen microbes might have been adapted sufficiently to mask any decrease in DM or fibre digestibility caused due to sudden exposure to monensin.
So, it can be concluded that 21 d adaptation to the donor cattle with monensin can mask the inhibitory effect of monensin on in vitro DM and fibre digestibility and VFA production and 35 ppm monensin level is optimum when supplemented with UMMB to increase in vitro propionate production and reduce butyrate and methane production.
Gas production can serve as an index of rumen microbial activity. These results thus indicated that rumen microbial activity was not adversely affected due to monensin treatment as no difference in gas production was observed after 14 d of adaptation with monensin.
When overall means of four periods were considered, it was found that methane production was lowest (p<0.01) at 35 ppm monensin enriched UMMB treatment and it was higher (p<0.01) in concentrate supplemented wheat straw treatment as compared to that of all monensin enriched UMMB supplemented wheat straw treatments.
However, no significant difference in methane production was observed among 70, 100, 150 and 200 ppm monensin level. When overall means of four periods were considered, it was found that methane production was lowest (p<0.01) at 35 ppm monensin enriched UMMB treatment and it was higher (p<0.01) in concentrate supplemented wheat straw treatment as compared to that of all monensin enriched UMMB supplemented wheat straw treatments. Average methane production (%) of P I was lower (p<0.

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DOI : 10.5713/ajas.2005.320 [무료]
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