[논문]Effects of Chromium Propionate Supplementation on Growth Performance, Serum Traits and Immune Response in Weaned Pigs

Lien, T.-F.; Yang, K.-H.; Lin, K.-J.

doi:10.5713/ajas.2005.403

Effects of Chromium Propionate Supplementation on Growth Performance, Serum Traits and Immune Response in Weaned Pigs 원문보기

Asian-Australasian journal of animal sciences, v.18 no.3, 2005년, pp.403 - 408

Lien, T.-F. (Department of Animal Science, National Chiayi University) , Yang, K.-H. (Department of Veterinary medicine, National Chiayi University) , Lin, K.-J. (Department of Animal Science, National Chiayi University)

Abstract ▼ AI-Helper

This study investigated the effects of chromium propionate on growth performance, serum traits and immune response in weaned pigs. Twenty-four 4 wk-old crossbred weanling pigs (initial body weight about 9.52${\pm}$0.48 kg) were randomly allotted into one of two groups, a control group (basal diet), chromium propionate group (diet supplemented with 200 ${\mu}g$ $kg^{-1}$ (ppb) of chromium propionate). This experiment was conducted over nine weeks. Escherichia coli lipopolysaccharide (LPS) 100 ${\mu}g$ $kg^{-1}$ BW was used as the stress-inducing agent in the middle (4 wks) and final (8 wks) periods. The experimental results indicated that chromium propionate had no effect on growth performance (p>0.05). Chromium propionate supplementation reduced the percentage of LDL+VLDL (low and very low-density lipoprotein) and increased HDL (high-density lipoprotein), but did not affect other serum traits. Pigs supplemented with chromium propionate had higher antibody titers specific for sheep red blood cells (SRBC) and serum total globulin relative to the control during the final period (p<0.05). A challenge with LPS increased white blood cells in the chromium propionate group in both experimental periods (p<0.05). The chromium propionate group exhibited higher IgG and $\gamma$-globulin than the control during the middle experimental period (p<0.05). Moreover, the PHA (phytohemagglutinin) challenge result in the chromium propionate group was better than the control group (p=0.056). Greater neutrophil activity was displayed than in the control (p<0.05). This suggests that chromium propionate supplementation benefited the weaned pigs in lipoprotein and immune response.

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문제 정의

, 1993). Thus, the objective of this work was to investigate the chromium supplementation on the immune response of normal and stress conditions ofweaned pigs.
, 1993). Thus, the objective of this work was to investigate the chromium supplementation on the immune response of normal and stress conditions ofweaned pigs.

제안 방법

Escherichia coli lipopolysaccharide (LPS), an endotoxin, was used as the stress-inducing agent at dose of 100 μg kg BW-1 injection by i.m. during the middle (4 wks) and final (8 wks) periods of the experiment.
(1997) in duplicate. For measure antibodies against sheep red blood cell (SRBC), the SRBC was isolated from black belley sheep, and 2 ml, 0.25% SRBC was injected two times at 5 and 6 week of the experiment, the blood samples was taken at 7 week. Antibodies titer against SRBC was measured in duplicate followed application of the Van Heugten et al.
, 1995). Therefore, this study took measurements at 6 h and 24 h after LPS administration in the final and middle period of the experiment. The number of white blood cells in the chromium propionate group increased markedly in both periods.
In a previous investigation (unpublished), we confirmed that chromium propionate could be as effective on serum traits as chromium picolinate in pigs. This work, therefore, investigates organic chromium propionate supplementation effects on growth, serum traits and immune response in normal and stress condition of weaned pigs.

데이터처리

The SAS system was used to analyze the variance between groups, with the significance determined with T test (SAS, 1990). According to the model, Cr was the main effect.

이론/모형

25% SRBC was injected two times at 5 and 6 week of the experiment, the blood samples was taken at 7 week. Antibodies titer against SRBC was measured in duplicate followed application of the Van Heugten et al. (1997) procedure for investigating humoral immune response. To examine the mitogen of phytohemagglutinin (PHA) skin swelling response, 150 μg ml^-1 PHA was injected at 7 weeks of the experiment by s.
Neutrophil activity was examined at final period of experiment as described by Morrow-Tesch and Andersson (1994) to determine cellular immune response. Serum immunoglobulin G (IgG) was determined in triplicate at 4 and 8 weeks of experiment using the method described by Leslie and Frank (1989), with purified porcine IgG used as the standard.
Neutrophil activity was examined at final period of experiment as described by Morrow-Tesch and Andersson (1994) to determine cellular immune response. Serum immunoglobulin G (IgG) was determined in triplicate at 4 and 8 weeks of experiment using the method described by Leslie and Frank (1989), with purified porcine IgG used as the standard.

성능/효과

The experimental results from this study indicated that chromium propionate supplementation could reduce LDL+VLDL and increase HDL percentage. Moreover, it can enhance serum total globulin, IgG and the y-globulin concentration.
This study found that chromium propionate supplementation did not improve pig growth performance. This was consistent with our previous study with chromium picolinate (Lien et al.
Generally, neutrophils are the first cells to respond followed by monocytes and lymphocytes. This study showed that the chromium propionate group had the greater PHA skin fold thickness. Meanwhile, the neutrophils activity test also showed that the chromium propionate group was a greater response than the control group.

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