[논문]Effect of Restrict Feeding, Roxarsone or Its Analogues in Inducing Fatty Livers in Mule Ducks

Chen, Kuo Lung; Chiou, Peter W.S.

doi:10.5713/ajas.2005.241

Effect of Restrict Feeding, Roxarsone or Its Analogues in Inducing Fatty Livers in Mule Ducks 원문보기

Asian-Australasian journal of animal sciences, v.18 no.2, 2005년, pp.241 - 248

Chen, Kuo Lung (Dept. of Animal Science, National Chia-Yi University) , Chiou, Peter W.S. (Dept. of Animal Science, National Chia-Yi University)

Abstract ▼ AI-Helper

This study is aimed at understanding the role of arsenic in Roxarsone in causing fatty livers in mule ducks. One hundred 10-week-old mule ducks were randomly divided into 5 groups. Ducks received 2 weeks of various treatments followed by 2 weeks of withdrawal. The treatments were non-treatment (control), 300 mg/kg Roxarsone inclusion for 2 weeks ($1^{st}$ and $2^{nd}$ week), Roxarsone inclusion for one week ($2^{nd}$ week only), restrict feeding, or Roxarsone analogue (3-nitro-4-hydroxyphenyl acid) inclusion. Results showed that feed intake and body weight in the Roxarsone groups and the restrict feeding group decreased significantly during the treatment period. However only the liver and heart weights were significantly decreased (p<0.05) in the restrict feeding group. Fatty acid synthetase (FAS) activity showed a significant decrease (p<0.05) in the Roxarsone groups and the restrict feeding group, two-week-Roxarsone treatment significantly increased NADP-malic dehydrogenase (MDH) activity compared to the restrict (p<0.05). After 2 weeks drug withdrawal, the 1-week-Roxarsone or restrict feeding group showed significantly increased (p<0.05) glucose-6-phosphate dehydrogenase (G-6-PDH) activity (p<0.05). Two-week-Roxarsone treatment significantly decreased (p<0.05) the high density lipoprotein (HDL) and increased (p<0.05) the low density lipoprotein (LDL) and very low density lipoprotein (VLDL) ratio. After drug withdrawal, the 1-week-Roxarsone or restrict feeding group showed significantly increased (p<0.05) creatine kinase (CK) activity. The 2-week-Roxarsone treatment group showed significantly increased (p<0.05) aspartate aminotransferase (AST) activity. The restrict feeding treatment group showed significantly decreased (p<0.05) total protein (TP) concentration. After drug withdrawal, the related enzyme activities in the blood that reflected the liver function were restored to the normal physiological range, except for the total bilirubin concentration and CK activity in the 1-week-Roxarsone group. This group showed a significant increase (p<0.05). Thus, the reasons for liver enlargement in the Roxarsone and restrict feeding groups were different.

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가설 설정

This creates a hepatic lipid synthesis imbalance in poultry. 2) The toxic effect of Roxarsone induces abnormal hepatic lipid synthesis and/or depresses lipid catabolism. This decreases hepatic lipid removal, causing accumulated lipids and fatty livers.

제안 방법

To understand the role of arsenic in fatty liver formation in mule ducks, the experimental treatments included Roxarsone, its’ analogues, the analogue (3-nitro-4-hydroxyphenyl acid) without arsenic content, and restrict feed intake to the same level during Roxarsone (300 mg/kg) treatment. This experiment was designed to clarify arsenic toxicity, the chemical structure of Roxarsone and its effect on the feed intake that causes fatty livers in mule ducks.
To understand the role of arsenic in fatty liver formation in mule ducks, the experimental treatments included Roxarsone, its’ analogues, the analogue (3-nitro-4-hydroxyphenyl acid) without arsenic content, and restrict feed intake to the same level during Roxarsone (300 mg/kg) treatment.
To understand the role of arsenic in fatty liver formation in mule ducks, the experimental treatments included Roxarsone, its’ analogues, the analogue (3-nitro-4- hydroxyphenyl acid) without arsenic content, and restrict feed intake to the same level during Roxarsone (300 ㎎/㎏) treatment.

이론/모형

A completely randomized design was applied to examine the effects of the arsenic levels or chemical forms. Analyses of variance were calculated using the general linear model principles and procedures of the SAS (1984). Duncan’s new multiple-range test was used to compare the means according to Steel and Torrie (1960).

성능/효과

05) in only the restrict feeding group compared to the other treatment groups. After returning to the basal diet for one week, both Roxarsone and restrict feeding groups showed significantly increased (p[0.05) feed intake, liver and relative liver weight over the other groups. The live weight returned to the level of the control group without significant differences from each other (p]0.
Table 4 presents the effect of Roxarsone dietary inclusion, its analogue or restrict feeding on the serum lipid and lipoprotein in mule ducks. The 2-week Roxarsone treatment significantly decreased the serum HDL ratio (p<0.05) and increased VLDL and LDL ratio (p[0.05). However, no significant difference (p]0.

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