Obesity can be defined as a metabolic disease due to an increased fat accumulation in the body caused by an imbalance of calorie intake and output The prevalence of obesity has increased substantially over the past 2-3 decades in developed and developing countries. The health impact of weight gain i...
Obesity can be defined as a metabolic disease due to an increased fat accumulation in the body caused by an imbalance of calorie intake and output The prevalence of obesity has increased substantially over the past 2-3 decades in developed and developing countries. The health impact of weight gain is so marked that obesity has now been classified as a major global public health problem In order to investigate the effect of diet conversion and oral administration of Platycodon grandiflorum extracts on the treatment of obesity, male Spraque-Dawley rats were divided into four groups: a group converted to normal diet (Control group), a group maintained high fat (30%) diet (H), and two groups with Platycodon grandiflorum extract added to the previously mentioned two groups. All animals were fed high fat diet for 7 weeks to induce the obesity. Then they were divided as mentioned above. Animals were fed experimental diet and Platycodon grandiflorum extract (150 mg/ml/rat/day) for 7 weeks. Body weight, adipose tissue weight (subcutaneous, epididymal, peritoneal fat pads) and serum lipids (total cholesterol and triglyceride) showed some differences among groups. The Platycodon grandiflorum feeding markedly decreased both body weight and adipose tissue weight in control group compared to H, high fat diet maintaining, group. Platycodon grandiflorum extracts significantly decreased the concentrations of serum lipids compared to H group. Fat cell numbers and sizes were significantly reduced in the oriental medicinal herb extract administrated group. Increased fatty acid binding protein (FABP) expression in high fat diet group was decreased by the dietary conversion to normal diet and the oral administration of Platycodon glandiflorum extracts. In contrast, there was no significant effect on FABP expression in the high fat maintenance group. In this study, the conversion from high fat diet to low fat or normal diet had a beneficial effect on body weight loss and serum lipid profiles. Dietary Platycodon glandiflorum extracts had an additive beneficial effect on the prevention and treatment of obesity.
Obesity can be defined as a metabolic disease due to an increased fat accumulation in the body caused by an imbalance of calorie intake and output The prevalence of obesity has increased substantially over the past 2-3 decades in developed and developing countries. The health impact of weight gain is so marked that obesity has now been classified as a major global public health problem In order to investigate the effect of diet conversion and oral administration of Platycodon grandiflorum extracts on the treatment of obesity, male Spraque-Dawley rats were divided into four groups: a group converted to normal diet (Control group), a group maintained high fat (30%) diet (H), and two groups with Platycodon grandiflorum extract added to the previously mentioned two groups. All animals were fed high fat diet for 7 weeks to induce the obesity. Then they were divided as mentioned above. Animals were fed experimental diet and Platycodon grandiflorum extract (150 mg/ml/rat/day) for 7 weeks. Body weight, adipose tissue weight (subcutaneous, epididymal, peritoneal fat pads) and serum lipids (total cholesterol and triglyceride) showed some differences among groups. The Platycodon grandiflorum feeding markedly decreased both body weight and adipose tissue weight in control group compared to H, high fat diet maintaining, group. Platycodon grandiflorum extracts significantly decreased the concentrations of serum lipids compared to H group. Fat cell numbers and sizes were significantly reduced in the oriental medicinal herb extract administrated group. Increased fatty acid binding protein (FABP) expression in high fat diet group was decreased by the dietary conversion to normal diet and the oral administration of Platycodon glandiflorum extracts. In contrast, there was no significant effect on FABP expression in the high fat maintenance group. In this study, the conversion from high fat diet to low fat or normal diet had a beneficial effect on body weight loss and serum lipid profiles. Dietary Platycodon glandiflorum extracts had an additive beneficial effect on the prevention and treatment of obesity.
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문제 정의
This study was performed to investigate the effect of dietary conversion and Platycodon glandiflorum extract on obesity treatment and improvement.
제안 방법
After the one-week of adaptation period, the experimental diet was given for 7 weeks along with the oral administration of hot water extract of Platycodon glandiflorum to examine the effect of the oral administration of Platycodon glandiflorum on the improvement of dietary obesity.
Based on the above experimental results, this study was performed to investigate the effect of shifting to the low-calorie diet on the improvement or treatment of obesity in high-fat diet induced obesity, and also to investigate the effect of simultaneous administration of Platycodon glandiflorum, which has been used in the treatment of obesity using Platycodon glandiflorum extract, on the FABP expression in adipose tissues.
Blood samples were obtained from the tail vein of rats at the 0, 2, 4 and 7 week (the last day) of the experiment and centrifuged. Plasma samples obtained from the centrifugation were used as samples for blood lipid concentration analysis.
Dietary obesity was induced by providing high fat diet for 7 weeks and then animals were divided into two groups in which one group was shifted to normal diet and the other group was maintained on the high fat diet for another 7 weeks. Experimental groups in this study were divided into four groups such as a group maintained on high fat diet, a group shifted from high fat diet to normal diet, and two groups with the addition of Platycodon glandiflorum extract to the previously mentioned two groups. The composition of experimental diets was shown in Table 1.
Among adipose tissues, the cell number and cell area of epididymal adipose tissue were measured. Frozen epididymal adipose tissue was thinly sliced serially in depth with 40 iim by using freezing microtome (cryostate) and stained with Mayer5 s hematoxylin, and then the cell area was measured by using light microscopy and image analysis program.
대상 데이터
Experimental animals used in this study were 14-week old male Sprague-Dawley rats (Dae-Han Bio Link) with the average body weight of 438.04±3.09 g for the first experimental design and 7-week old male rats with the average body weight of 318.43±3.80 g for the second experimental design. These experimental animals were housed two per metabolic cage and maintained with regular feeding management everyday during the experimental period.
Oriental medicinal herb used in this experiment was Platycodon glandiflorum that was approved as a foodstuff among Oriental medicinal ingredients used for the treatment of obesity and preliminary experiments. Platycodon glandiflorum was obtained from products of Yeongcheon-gun, Gyeongsangbuk-do. Platycodon glandiflorum was extracted in 5 times volume of distilled water for 2 hours for the first extraction, and then the collected extract was mixed again with 5 times volume of distilled water and extracted for 2 hours for the second extraction.
데이터처리
The significance test for analyzed values was performed by using one way- ANOVA. Duncan's multiple-range test was performed on analyzed values at the level of p<0.05 to analyze the significant differences among average values of each treatment group.
Statistical analysis for experimental results was performed by SPSS program and all measurements were expressed as Mean±SE. The significance test for analyzed values was performed by using one way- ANOVA.
Statistical analysis for experimental results was performed by SPSS program and all measurements were expressed as Mean±SE. The significance test for analyzed values was performed by using one way- ANOVA. Duncan's multiple-range test was performed on analyzed values at the level of p<0.
성능/효과
At the beginning of the experiment, the average body weight of 7-week old rats was 318.43±3.80g and significantly increased to 516.19±5.98 g after 7 weeks of high fat diet. These obese rats were randomly selected and grouped for the following experiment.
Form the above results, it was understood that Platycodon glandiflorum could suppress the FABP expression during the developmental process of obesity, but no longer had the inhibitory effect on the FABP expression after the obesity was fully developed because FABP had already expressed.
It means that, the Platycodon glandiflorum extract had inhibitory effect on the fat accumulation. The change in serum lipid concentrations showed that the dietary conversion from high fat diet to normal diet was helpful in the improvement of serum lipid concentrations, and also the extract of Platycodon glandiflorum was helpful in serum lipid lowering effect.
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