[논문]Identification of Glycine max Genes Expressed in Response to Soybean mosaic virus Infection

Jeong, Rae-Dong; Lim, Won-Seok; Kwon, Sang-Wook; Kim, Kook-Hyung

doi:10.5423/ppj.2005.21.1.047

Identification of Glycine max Genes Expressed in Response to Soybean mosaic virus Infection 원문보기

The plant pathology journal, v.21 no.1, 2005년, pp.47 - 54

Jeong, Rae-Dong (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) , Lim, Won-Seok (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) , Kwon, Sang-Wook (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) , Kim, Kook-Hyung (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)

Abstract ▼ AI-Helper

Identification of host genes involved in disease progresses and/or defense responses is one of the most critical steps leading to the elucidation of disease resistance mechanisms in plants. Soybean mosaic virus (SMV) is one of the most prevalent pathogen of soybean (Glycine max). Although the soybeans are placed one of many important crops, relatively little is known about defense mechanism. In order to obtain host genes involved in SMV disease progress and host defense especially for virus resistance, two different cloning strategies (DD RT-PCR and Subtractive hybridization) were employed to identify pathogenesis- and defenserelated genes (PRs and DRs) from susceptible (Geumjeong 1) and resistant (Geumjeong 2) cultivars against SMV strain G7H. Using these approaches, we obtained 570 genes that expressed differentially during SMV infection processes. Based upon sequence analyses, differentially expressed host genes were classified into five groups, i.e. metabolism, genetic information processing, environmental information processing, cellular processes and unclassified group. A total of 11 differentially expressed genes including protein kinase, transcription factor, other potential signaling components and resistant-like gene involved in host defense response were selected to further characterize and determine expression profiles of each selected gene. Functional characterization of these genes will likely facilitate the elucidation of defense signal transduction and biological function in SMV-infected soybean plants.

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가설 설정

Open and closed arrowheads indicate selected cDNAs for further molecular analysis. B, The selected cDNAs from differential display were reamplified and cloned into pGEM-T easy vector. The first lane represent DNA molecular mass marker Z-DNA digested with Hzwdlll.

제안 방법

The deduced amino acid sequences from nucleotide Table 3. Characteristics of cloned cDNAs fragment identified by DDRT-PCR and subtracted hybridization sequences of each EST clones were applied to BLASTX analyses to examine their homology with functional genes in the GenBank database. Several clones contained significant sequence homology to genes with interesting functions such as defender against cell death, drought induced protein, and calcium-dependent protein kinase.
Subcloning, sequencing and sequence analysis. DNA sequencing for the differentially expressed cDNA clones and subtracted cDNA clones in pGEM-T vector were performed with the BigDye Terminator Cycle Sequencing Kits (PE Biosystems, USA) using an automated DNA sequencer (ABI Prism 3700 Genetic Analyzer, Perkin Elmer, USA) at the NICEM (SNU). Primers used for sequencing were M13 F (5'-TGTAAAACGACCGC- CAGT-3') and M13 R (S-AACAGCTATGACCATG-3).
The synthesized cDNAs were completely digested with Rsal and subjected to PCR-based subtractive hybridization by the standard protocol (Clontech, USA). cDNAs obtained from Geumjeong 1 and Geumjeong 2 were used as driver and tester, respectively The subtracted cDNAs were finally cloned into pGEM-T easy vector (Promega, USA) and subjected to further analysis.

대상 데이터

A total of 570 clones (96 clones from PCR-based subtracted hybridization and 474 clones from DD RT-PCR approaches) were selected and sequenced. To prevent isolation of false positive, all amplification experiments were performed on two different total RNAs extracted from separately inoculated plants.

이론/모형

Upper uninoculated leaves of SMV-G7H-infected soybean plants were harvested and stored at -70℃ for RNA isolation at 7 days post-inoculation (dpi). Tot시 RNAs were extracted using TriZol™ reagent (Giboco BRL, USA) according to the manufoctxire's protocol. Total RNAs were treated with DNase I (TaKaRa, Japan) to remove the genomic DNA contamination.

성능/효과

Zinc finger protein and DADI have been reported to function in plants defense against diseases and in controlling plant programmed cell death through the N- linked glycosylation (Crosti et al, 2001; Klug, 1999; Rushton and Somssich, 1998). The amino acid sequences of cDNA clones SS15 and SS17 showed 80% and 88% similarities to dehydration stress-induced protein of A. thaliana and aquaporin protein of Brassica rapa, respectively. These genes were shown to be primary related with plant-environment and with the water-channel activity during tomato-microbes interaction (Wemer et al.
2). The average sizes of cDNAs of obtained from Subtracted hybridization and DD RT-PCR were approximately 300 bp to 650 bp, indicating that the cDNAs contain enough information for sequence analysis. Tlie nucleotide sequences of the differentially expressed cDNA clones were determined and were searched further compared with sequences deposited in public database (CCGB, Soybean EST GenBank; http://soybean.
The cDNA DSG57 encodes a part of putative TFⅢA (or kruppel)-like zinc finger protein of Medicago sativa. The deduced amino acid sequences showed 70% similarity while the deduced amino acid sequence of cDNA DSG65 아)owed 89% similarity to DAD 1 of Arabidopsis thaliana. Zinc finger protein and DADI have been reported to function in plants defense against diseases and in controlling plant programmed cell death through the N- linked glycosylation (Crosti et al, 2001; Klug, 1999; Rushton and Somssich, 1998).

참고문헌 (45)

Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402

상세보기
Andrejeva, J., Puurand, U., Merits, A., Rabenstein, F., Jarvekulg, L. and Valkonen, J. P. 1999. Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus-host interactions and the same CP motif affects virus transmission and accumulation. J. Gen. Virol. 80:1133-1139

상세보기
Browning, K. S. 2004. Plant translation initiation factors: it is not easy to be green. Biochem. Soc. Trans. 32:589-591

상세보기
Carginale, V., Maria, G., Capasso, C., Ionata, E., La Cara, F., Pastore, M., Bertaccini, A. and Capasso, A. 2004. Identification of genes expressed in response to phytoplasma infection in leaves of Prunus armeniaca by messenger RNA differential display. Gene 332:29-34

상세보기
Cho, E. K., Choi, S. H. and Cho, W. T. 1983. Newly recognized soybean mosaic virus mtants and sources of resistance in soybean. Res. Rept. ORD (S.P.M.U.) 25:18-22
Cho, E. K. and Goodman, R. M. 1979. Strains of Soybean mosaic virus: classification based on virulence in resistant soybean cultivars. Phytopathology. 69:467-470

상세보기
Cho, E. K. and Goodman, R. M. 1982. Evaluation of resistance on soybeans to Soybean mosaic virus strains. Crop Sci. 22:1133- 1136

상세보기
Crosti, P., Malerba, M. and Bianchetti, R. 2001. Tunicamycin and Brefeldin A induce in plant cells a programmed cell death showing apoptotic features. Protoplasma 216:31-38

상세보기
Czernic, P., Visser, B., Sun, W., Savoure, A., Deslandes, L., Marco, Y., Van Montagu, M. and Verbruggen, N. 1999. Characterization of an Arabidopsis thaliana receptor-like protein kinase gene activated by oxidative stress and pathogen attack. Plant J. 18:321-327

상세보기
Dempsey, D. A. and Klessig, D. F. 1994. Salicylic acid, active oxygen species and systemic acquired resistance in plants. Trends Cell Biol. 4:334-338

상세보기
Diatchenko, L., Lau, Y. F., Campbell, A. P., Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, E. D. and Siebert, P. D. 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93:6025-6030
Dixon, R. A. and Lamb, C. J. 1990. Molecular communication in interactions between plants and microbial pathogens. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41:339-367

상세보기
Dixon, R. A., Harrison, M. J. and Lamb, C. J. 1994. Early events in the activation of plant defense responses. Annu. Rev. Phytopathol. 32:479-501

상세보기
Ellis, J., Dodds, P. and Pryor, T. 2000. Structure, function and evolution of plant disease resistance genes. Curr. Opin. Plant Biol. 3:278-284

상세보기
Flor, H. H. 1971. Current status of the gene-for-gene concept. Annu. Rev. Phytopathol. 9:275-296

상세보기
Gao, Z., Johansen, E., Eyers, S., Thomas, C. L., Noel Ellis, T. H. and Maule, A. J. 2004. The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. Plant J. 40:376-385

상세보기
Gunduz, I., Buss, G. R., Ma, G., Chen, P. and Tolin, S. A. 2001. Genetic analysis of resistance to Soybean mosaic virus in OX670 and Harosoy soybean. Crop Sci. 41:1785-1791

상세보기
Gunduz, I., Buss, G. R., Chen, P. and Tolin, S. A. 2002. Characterization of SMV resistance genes in Tousan 140 and Hourei soybean. Crop Sci. 42:90-95

상세보기
Graham, M. A., Mark, L. F. and Shoemarker, R. C. 2002. Organization, expression and evolution of a disease resistance gene cluster in soybean. Genetics. 162:1961-1977

상세보기
Hammond-kosack, K. E. and Jones, J. D. 1996. Resistance geneindependent plant defense responses. Plant Cell. 8:1773-1791

상세보기
Hayes, A. J., Ma, G., Buss, G. R. and Maroof, M. A. 2000. Molecular marker mapping of RSV4, a gene conferring resistance to all known strains soybean mosaic virus. Crop Sci. 40:1434-1437

상세보기
He, C. Y., Tian, A. G., Zhang, J. S., Zhang, Z. Y., Gai, J. Y. and Chen, S. Y. 2003. Isolation and characterization of a fulllength resistance gene homolog from soybean. Ther. Appl. Genet. 106:786-793

상세보기
Jagoueix-Eveillard, S., Tarendeau, F., Guolter, K., Danet, J. L., Bove, J. M. and Garnier, M. 2001. Catharanthus roseus genes regulated differentially by mollicute infections. Mol. Plant-Microbe Interact. 14:225-233

상세보기
Jeong, S. C., Kristipati, S., Hayes, A. J., Maughan, P. J., Noffsinger, S. L., Gunduz, I. and Maroof, M. A. 2002. Genetic and sequence analysis of markers tightly linked to the soybean mosaic virus resistance gene, Rsv3. Crop Sci. 42:265-270

상세보기
Kanazin, V., Marek, L. F., Shoemaker, R. C. 1996. Resistance gene analogs are conserved and clustered in soybean. Proc. Natl. Acad. Sci. USA 93:11746-11750
Keen, N. T. 1990. Gene-for-gene complementarity in plant-pathogen interactions. Annu. Rev. Genet. 24:447-463

상세보기
Kim, Y.-H., Kim, O.-S., Lee, B.-C., Moon, J.-K., Lee, S.-C. and Lee, J.-Y. 2003. G7H, a new Soybean mosaic virus strain: its virulence and nucleotide sequence of CI gene. Plant Dis. 87:1372-1375

상세보기
Klug, A. 1999. Zinc finger peptides for the regulation of gene expression. J. Mol. Biol. 293:215-218

상세보기
Leister, D., Ballvora, A., Salamini, F. and Gebhardt, C. 1996. A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nat. Genet. 14:421-429

상세보기
Liang, P. and Pardee, A. B. 1998. Differential display: A general protocol. Mol. Biotechnol. 10:261-267

상세보기
Lim, S. M. 1985. Resistance to Soybean mosaic virus in soybean. Phytopathology. 79:649-657
Lim, W.-S., Kim, Y.-H. and Kim, K.-H. 2003. Complete genome sequence of the genomic RNA of Soybean mosaic virus strains G7H and G5. Plant Pathol. J. 19:171-176

원문보기 상세보기
Logemann, E., Wu, S. C., Schroder, J., Schmelzer, E., Somssich, I. E. and Hahlbrock, K. 1995. Gene activation by UV light, fungal elicitor or fungal infection in Petroselinum crispum is correlated with repression of cell cycle-related genes. Plant J. 8:865-876

상세보기
Martin, G. B., Brommonschenkel, S. H., Chunwongse, J., Frary, A., Ganal, M. W., Spivey, R., Wu, T., Earle, E. D. and Tanksley, S. D. 1993. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432-1436

상세보기
May, M. A. and Pringle, C. R. 1998. Virus taxonomy-1997. J. Gen. Virol. 79:649-657

상세보기
Nadim, W. A. and Benjamin, F. M. 2004. SGMD: the Soybean Genomic and Microarray Database. Nucl. Acids Res. 32:398- 400

상세보기
Piffanelli, P., Devoto, A. and Schulze-Lefert, P. 1999. Defence signalling pathways in cereals. Curr. Opin. Plant Biol. 2:295- 300

상세보기
Rushton, P. J. and Somssich, I. E. 1998. Transcriptional control of plant genes responsive to pathogens. Curr. Opin. Plant Biol. 1:311-315

상세보기
Song, W. Y., Wang, G. L., Chen, L. L., Kim, H. S., Pi, L. Y., Holsten, T., Gardner, J., Wang, B., Zhai, W. X. and Zhu, L. H. 1995. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270:1804-1806

상세보기
Staskawicz, B. J. 2001. Genetics of plant-pathogen interactions specifying plant disease resistance. Plant Physiol. 125:73-76

상세보기
van der Hoeven, R., Ronning, C., Giovannoni, J., Martin, G. and Tanksley, S. 2002. Deductions about the number, organization, and evolution of genes in the tomato genome based on analysis of a large expressed sequence tag collection and selective genomic sequencing. Plant Cell 14:1441-1456

상세보기
Whitham, S., Dinesh-Kumar, S. P., Choi, D., Hehl, R., Corr, C. and Baker, B. 1994. The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell. 78:1101-1115

상세보기
Werner, M., Uehlein, N., Proksch, P. and Kaldenhoff, R. 2001. Characterization of two tomato aquaporins and expression during the incompatible interaction of tomato with the plant parasite Cuscuta reflexa. Planta 213:550-555

상세보기
Yang, Y., Shah, J. and Klessig, D. F. 1997. Signal perception and transduction in plant defense responses. Genes Dev. 11:1621-1639

상세보기
Yu, Y. G., Maroof, M. A., Buss, G. R., Maughan, P. J. and Tolin, S. A. 1994. RFLP and microsatellite mapping of a gene for soybean mosaic virus resistance. Phytopathology 84:60-64

상세보기

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