Hong, Yeon-Kyu
(Plant Environment Division, Yeongnam Agricultural Research Institute, RDA)
,
Lee, Bong-Choon
(Plant Environment Division, Yeongnam Agricultural Research Institute, RDA)
,
Song, Seok-Bo
(Plant Environment Division, Yeongnam Agricultural Research Institute, RDA)
,
Hwang, Jae-Bok
(Plant Environment Division, Yeongnam Agricultural Research Institute, RDA)
,
Park, Sung-Tae
(Plant Environment Division, Yeongnam Agricultural Research Institute, RDA)
A summer annual weed of monochoria (Monochoria vaginalis) grows in the edges of rice paddies, ditches, and moist upland throughout Korea. It is very difficult to control with herbicide because of its sulfonylurea resistance. It is very competitive with fast growing pattern, that can cause reducing y...
A summer annual weed of monochoria (Monochoria vaginalis) grows in the edges of rice paddies, ditches, and moist upland throughout Korea. It is very difficult to control with herbicide because of its sulfonylurea resistance. It is very competitive with fast growing pattern, that can cause reducing yields of rice. Brown leaf blight of monochoria (Monochoria vaginalis) occurred naturally in rice paddy, is first reported in Korea. The fungal isolate BWC01-54 was successfully isolated from the diseased leaves of monochoria. The fungus BWC 01-54 was grown well at $25-28^{\circ}C$, conidia of the greysh black brown mycelia were abundant produced on PDA at 15 days. The fungus was grown well in potato dextrose broth at $28^{\circ}C$ and fully grown within 10 days in 250 ml of flask. In host and pathogenicity test, conidia suspension of BWC01-54 was the most effective to control of monochoria compare to others isolates. Typical symptoms having pin point brown lesions were formed on stem and leaf and which severely affected the whole plants ware blighted within two weeks, respectively. Under paddies field condition, conidial suspension of the fungus BWC01-54 gave around 90% control. Therefore, we conclude that the fungus may have a potential as a biological control agent against sulfonylurea resistance weed in rice paddy.
A summer annual weed of monochoria (Monochoria vaginalis) grows in the edges of rice paddies, ditches, and moist upland throughout Korea. It is very difficult to control with herbicide because of its sulfonylurea resistance. It is very competitive with fast growing pattern, that can cause reducing yields of rice. Brown leaf blight of monochoria (Monochoria vaginalis) occurred naturally in rice paddy, is first reported in Korea. The fungal isolate BWC01-54 was successfully isolated from the diseased leaves of monochoria. The fungus BWC 01-54 was grown well at $25-28^{\circ}C$, conidia of the greysh black brown mycelia were abundant produced on PDA at 15 days. The fungus was grown well in potato dextrose broth at $28^{\circ}C$ and fully grown within 10 days in 250 ml of flask. In host and pathogenicity test, conidia suspension of BWC01-54 was the most effective to control of monochoria compare to others isolates. Typical symptoms having pin point brown lesions were formed on stem and leaf and which severely affected the whole plants ware blighted within two weeks, respectively. Under paddies field condition, conidial suspension of the fungus BWC01-54 gave around 90% control. Therefore, we conclude that the fungus may have a potential as a biological control agent against sulfonylurea resistance weed in rice paddy.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
The purpose of this research was to isolate the fungus, to elucidate the host range of weed and weeding efficacy and to develop a successful biological control system of Monochoria (M. vaginalis), using fungal isolate BWC 01- 54.
5 m border line were prepared and planted with 100 plants. Treatments were arranged in a completely randomized block design with four replications. Rice (Sangjubyeo) was cultivated on end of May and other weeds were hand weeded.
대상 데이터
Experimental design. Field experiments were conducted at Yeongnam Agricultural Research Institute, Milyang. In 2001, 2 mx2 m plots, separated each other by 0.
Distribution of monochoria leaf blight disease in rice field. The disease occurred in all 18 counties surveyed for 2 years, and the causal fungus were isolated from Jangsoo, Jinan and Sangju. Frequency of the incidence, however, differed by rice area (Table 1).
, 1983). Three isolates used in this study were selected by their characteristics on solid agar among 30 isolates collected from diseased monochoria from 12 regions of fields in Korea. Isolates were characterized by the mycelial color.
성능/효과
2) which come to be an inoculum of next year's and can be suppressed new year around. Much differences were resulted from treatments with conidia suspension of isolate BWC01-54 and piperophos (4.4% ai.) (Fig. 4), The higher weeding efficacy occurred after treatment of conidia suspension, when treated with the piperophos (4.4% ai.) as a control showed 11% of plant mortality. This was lower than the treatment of conidia suspension of BWC01-54 73% within 10 days.
7% on plants among which BWC01-54 isolated from Jangsoo was the most virulent isolate. Other isolates, BWC00-95 and BWCO2-03 were less virulent, showing weeding efficacy of 31.5% and 36.7%, respectively. Infected shoots were killed in 10 to 15 days.
참고문헌 (34)
Aoshima, K., Chubagi, K. and Mirora, K. 1983. 菌類硏究法, 共立出版, Tokyo, Japan. p 61
Auld, B. A., Say, M. M., Ridings, H. I. and Andrews, J. 1990. Field applications of Colletotrichum orbiculare to control Xanthium spinosum. Agric. Ecosyst. Environ. 32:315-323
Danial, J. T., Templeton, G. E., Smith, R. J. and Fox, W. T. 1973. Biological control of northern joint vetch in rice with an endemic fungal disease. Weed Sci. 21:303-307
Elwakil, M. A., Sadik, E. A., Fayzalla, E. A. and Shabana, Y. M. 1990. Biological control of waterhyacinth with fungal plant pathogens in Egypt. Proc. 7th Int. Symp. Bio Control Weeds. E. S. Delfosse, ed. Rome, Italy. p. 483-498
Heap, I. 1997. The occurrence of herbicide-resistant weeds worldwide. Pestic. Sci. 51: 235-243
Heap, I. 2003. International survey of herbicide resistant weeds
Heap, I. 2002. International Survey of Herbicide Resistant. Weeds. http://www.weedscience.org/in.asp
Hong, Y. K. 1999. Biological control of water chestnut (Eleocharis kuroguwai) by an endemic host-specific pathogen (Epicoccosorus nematosporus) in rice field. Ph.D. Thesis Kyoungpook National University
Hong, Y. K., Cho, J. M., Kim, J. C. and Uhm, J. Y. 1996. Identification, pathogenicity and host range of a potential Epicoccosorus nematosporus, causing fingerprint stem blight on water chestnut, Eleocharis kuroguwai. Kor. J. Plant Pathol. 12:58-65
Hong, Y. K., Cho. J. M., Lee, B. C., Uhm, J. Y. and Kim, S. C. 2002a. Factors affecting sporulation, germination and appressoria formation of Epicoccosorus nematosporus as a mycoherbicide under controlled environment. Plant Pathol. J. 18:50- 53
Hong, Y. K., Hyun, J. N., Cho, J. M., Uhm, J. Y. and Kim, S. C. 2002b. Factors affecting sporulation of a mycoherbicide, Epicoccosorus nematosporus, on the lesion of Eleocharis kuroguwai. Plant Pathol. J. 18:81-84
Hong, Y. K., Cho, J. M., Ryu, K. L., Shin, D. B. and Kim, S. C. 2002c. The suitable cultural conditions for inoculum production of Epicoccosorus nematosporus as a mycoherbicide agent. Kor. J. Weed Sci. 22:61-66
Hong, Y. K., Ryu, K. L., Hyun, J. N., Uhm, J. Y. and Soon-Chul, Kim. 2002d. Distribution and alteration of fingerprint stem blight disease of Eleocharis kuroguwai caused by Epicoccosorus nematosporus, in Korea. Plant Pathol. J. 18:152-155
Hong, Y. K., Cho, J. M., Uhm, J. Y. and Ryu, K. R. 1997. Potential application of Epicoccosorus nematosporus for the control of water chestnut. Kor. J. Plant Pathol. 13:167-171
Hong, Y. K., Kim, J. C, Ryu, K. R. and Kim, S. C. 1991. Pathogenicity and some related characteristics of the fingerprint blight pathogen (Epicoccosorus nematosporus) attacking water chestnut (Eleocharis kuroguwai Ohwi). Kor. Plant Pathol. Newsletter 2:47 (Abstr.)
Hong, Y. K., Kim, J. C. and Lee, S. K. 1992. Biological control of rice weed, water chestnut (Eleocharis kuroguwai), using the fingerprint stem blight pathogen (Epicoccosorus nematosporus). Kor. Plant Pathol. Newletter 3:75 (Abstr.)
Hong, Y. K., Shin, D. B., Song, S. B., Lee, B. C. and Lee, D. C. 2001. Effect of some pesticides on the fungus Epicoccosorus nematosporus and synergistic effect in combination with herbicides on Eleocharis kuroguwai control in rice paddy field. Kor. J. Weed Sci. 21:365-372
Hong, Y. K., Song, S. B., Lee, D. C. and Kim, S. C. 2002e. Biological weed control with plant pathogenic microorganisms. Kor. J. Weed Sci. 22:1-14
Hong, Y. K., Song, S. B., Lee, B. C., Shin, D. B. and Lee, D. C. 2002f. Isolation of host specific fungal isolate YK201 to bulrush (Scirpus hotarui Ohwi) and weeding effect of the plants caused by natural infection in paddy field. Kor. J. Weed Sci. 22:55-60
Hong, Y. K., Cho, J. M., Uhm, J. Y., Lee, B. C., Hyun, J. N., Hwang, J. B. and Kim, S. C. 2003a. Evaluation of control efficacy of biocontrol agent, Epicoccosorus nematosporus in the field. Plant Pathol. J. 19:97-101
Hong, Y. K., Cho, J. M., Uhm, J. Y., Lee, B. C., Hyun, J. N., Song, S. B. and Lee, D. C. 2003b. Weeding efficacy of melanized formula with Epicoccosorus nematosporus on Eleocharis kuroguwai in the field. Plant Pathol.J. 19:92-96
Hong, Y. K., Song, S. B., Bae, S. D., Hwang, J. B., Park, S. T. and Kim, S. C. 2004a. Evaluation of mycelium virulence of mycoherbicide agent, fungal isolate BWC98-105 to Aeschynomene indica L. Kor. J. Weed Sci. 23:364-370
Hong, Y. K., Lee, B. C., Jung, W. K. Bae, S. D., Park, S. T. and Uhm, J. Y. 2004b. Isolation and partial characterization of phytotoxins produced by Sclerotinia sp., a potential bioherbicide for control of white clover (Trifoliorum repens). Plant Pathol. J. 20:52-57
Hong, Y. K., Cho, J. M., Lee, B. C., Song, S. B., Bae, S. D. and Park, S. T. 2004c. Pathogenicity and host range of a potential mycoherbicide, isolate BWC98-105, causing white root rot on Trifoliorum repens. Plant Pathol. J. 20:58-62
Itoh, K., Wang, G. X., Shibaike, H. and Matsuo, K. 1999. Habitat management and inhertance of sulfonylurea resistance in Lindernia micratha, an annual paddy weed in Japan. Proc. 17th APWSS Cong. 537-543, Bangkok
Kirkpatrick, T. L., Templeton, G. E., TeBeest, D. O. and Smith, R. J. Jr. 1982. Potential of Colletotrichum malvarum for biological control of prickly sida. Plant Dis. 66:323-325
Kohara, H., Konno, K. and Takekawa, M. 1999. Occurrence of sulfonylurea-resistant biotypes of Scirpus juncoides Roxb. var. ohwianus T. Koyama in paddy fields of Hokkaido perfecture, Japan. J. Weed Sci. &. Tech. 44:228-235
Makowski, R. M. D. and Mortensen, K. 1989. Colletotrichum gloeosporioides f. sp. malvae as a mycoherbicide for roundleaved mallow (Malvae pusilla): Conditions for successful control in the field. In: Proc. 7th Int. Symp. Biol. Control Weeds. ed. by E. S. Delfosse, pp. 513-522. Instituto Sperimentale per la Patologia Vegetale (Ministereo dell' Agricoltura e delle Foreste). Rome, Italy
Mortensen, K. 1988. The potential of an endemic fungus, Colletotrichum gloeosporioides, for biological control of roundleaved mallow (Malvae pusilla) and velvetleaf (Abutilon theophrasti). Weed Sci. 36:473-478
Ormeno-Nunez, J., Reeleder, F. D. and Watson, A. K. 1988. A foliar disease of field bindweed (Convolvulus arvensis) caused by Phomopsis convolvulus. Plant Dis. 72:338-342
Park, T. S., Kim, C. S., Park, J. P., Oh, Y. K. and Kim, K. U. 1999. Resistant biotype of Monochoria korsakowii against sulfonylurea herbicides in the reclaimed paddy fields in Korea. Proc. 17th APWSS p. 251-254. Bangkok
Suzuki, H. 1991. Biological control of a paddy weed, water chestnut, with fungal pahtogen. Bull. Hokuriku. Agri. Exp. Stn. 33:83-105
TeeBeest, D. O. and Templeton, G. E. 1985. Mycoherbicides: progress in the biological control of weeds. Plant Dis. 69:6-10
※ AI-Helper는 부적절한 답변을 할 수 있습니다.