Flowers of Erigeron annuus L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H$_2$O. Repeated silica gel and OD S column chromatography of the EtOAc fraction led to the isolation of a sterol, through activityguided fractionation,...
Flowers of Erigeron annuus L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H$_2$O. Repeated silica gel and OD S column chromatography of the EtOAc fraction led to the isolation of a sterol, through activityguided fractionation, using ACAT inhibitory activity measurements. From the physico-chemical data, including NMR, MS, and IR, the chemical structure of the compound was determined to be an ergosterol peroxide (1), which has been isolated for the first time from this plant. This compound exhibited hACAT-1 and Lp-PLA$_2$ inhibitory effects, with inhibitory values of 51.6 ${\pm}$ 0.9 and 51 .7 ${\pm}$ 1.2%, at a treatment concentration of 0.23 mM.
Flowers of Erigeron annuus L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H$_2$O. Repeated silica gel and OD S column chromatography of the EtOAc fraction led to the isolation of a sterol, through activityguided fractionation, using ACAT inhibitory activity measurements. From the physico-chemical data, including NMR, MS, and IR, the chemical structure of the compound was determined to be an ergosterol peroxide (1), which has been isolated for the first time from this plant. This compound exhibited hACAT-1 and Lp-PLA$_2$ inhibitory effects, with inhibitory values of 51.6 ${\pm}$ 0.9 and 51 .7 ${\pm}$ 1.2%, at a treatment concentration of 0.23 mM.
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대상 데이터
were collected by the authors from the fields and mountains of Ybngin, Kyunggi- Do, in August, 2002. A voucher specimen (KHU-02034) was reserved at the Laboratory of Natural Products Chemistry, KyungHee University, Suwon, Korea.
). The KH2PO4, dithiothreito), bovine serum albumin (fatty acid free) and all the reagent grade chemicals were purchased from Sigma-Aldrich.
The [1-14C)oleoyl-CoA (56.0 mCi/mmol, 50 jiCi/mL, CFA634) was purchased from Amersham Biosciences (Stockem, Sweden), the [3H]PAF (100 Ci/mL, 21.5 Ci/ mmol, NET 910) from Perkin Elmer Life Sciences, Inc, and the PAF(L-a・phosphatidylcholine, p-acetyl y-O- hexadecyl) from Sigma-Aldrich (St. Louis, U.S.A.). The KH2PO4, dithiothreito), bovine serum albumin (fatty acid free) and all the reagent grade chemicals were purchased from Sigma-Aldrich.
성능/효과
77) methyl signals were observed. In the 13C-NMR (100 MHz, CDCl3), twenty eight signals, consisting of four olefinic (8C 135.3, 135.1, 132.2, and 130.6), two oxygenated quaternary (6C 82.1 and 79.4) and one oxygenated methine (8C 66.4), as well as six further methine, seven methylene, two quaternary and six methyl (8C 20, 9, 20.0, 19.7, 18.2, 17.6, and 12.9) signals were observed, leading to the conclusion that compound 1 was a sterol, with two double bonds, three oxygenated carbons and four doublet and two singlet methyls. Compound 1 was finally identified as 5a, 8a-epidioxy-24(/?)-methylcho- lesta-6, 22-dien-3p-ol (ergosterol peroxide) by comparison of several physical and spectral data with those in the literature (Bok ef al.
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