$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

A new Bacillus peptidase, bacillopeptidase DJ-2 (bpDJ-2), with molecular mass of 42 kDa and isoelectric point (pI) of 3.5- 3.7, was purified to homogeneity from Bacillus sp. DJ-2 isolated from Doen-Jang, a traditional Korean soybean fermented food. The enzyme was identified as an extracellular serine fibrinolytic protease. The optimal conditions for the reaction were pH 9.0 and $60^{\circ}C$. The first 18 amino acid residues of the N-terminal amino acid sequence of bpDJ-2 were TDGVEWNVDQIDAPKAW, which is identical to that of bacillopeptidase F (bpf). However, based on their Nterminal amino acid sequence, molecular size, and pI, it is different from that of bpf and extracellular 90 kDa. The whole (2,541 bp, full-bpDJ-2) and mature (1,956 bp, mature-bpDJ-2) genes were cloned, and its nucleotide sequence and deduced amino acid sequence were determined. The expressed proteins, full-bpDJ-2 and mature-bpDJ-2, were detected on SDSPAGE at expected sizes of 92 and 68 kDa, respectively.

참고문헌 (31)

  1. Doi, R. H. 1983. In Recombinant DNA Techniques, pp. 162-163. R. L. Rodriguez and R.C. Tait (eds.), Addison-Wesley Publishing Co., MA, U.S.A. 
  2. Fujita, M., K. Nomura, K. Hong, Y. Ito, A. Asada, and S. Nishimuro. 1993. Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochem. Biophys. Res. Commum.197: 1340- 1347 
  3. Roitsch, C. A. and J. H. Hageman. 1983. Bacillopeptidase F: Two forms of a glycoprotein serine protease from Bacillus subtilis 168. J. Bacteriol. 155: 145-152 
  4. Sumi, H., H. Hamada, H. Tsushima, H. Mihara, and H. Muraki. 1987. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese natto; a typical and popular soybean food in the Japanese diet. Experientia 43: 1110- 1111 
  5. Sumi, H., N. Nakajima, and C. Yatagai. 1995. A unique strong fibrinolytic enzyme (Katsuwokinase) in Skipjack 'shiokara', a Japanese traditional fermented food. Comp. Biochem. Physiol. 112B: 543- 547 
  6. Tsuru, D., H. Kira, T. Yamamoto, and J. Fukumoto. 1966. Studies on bacterial protease. XlV. Zn as an essential metal component of neutral protease of Bacillus subtilis var. amylosacchariticus. Agric. Biol. Chem. 30: 1261-1268 
  7. Tsuru, D., H. Kira, T. Yamamoto, and J. Fukumoto. 1967. Studies on bacterial protease. XVII. Proteolytic specificity of neutral protease of Bacillus subtilis var. amylosacchariticus. Agric. Biol. Chem. 31: 718- 723 
  8. Yoon, S. J., M. A. Yu, G. S. Sim, S. T. Kwon, J. K. Hwang, J. K. Shin, I. H. Yeo, and Y. R. Pyun. 2002. Screening and characterization of microorganisms with fibrinolytic activity from fermented foods. J. Microbiol. Biotechnol. 12: 649-656 
  9. Robertson, E. E., K. Dannelly, P. J. Malloy, and H. C. Reeves. 1987. Rapid isoelectric focusing in a vertical polyacrylamide minigel system. Anal. Biochem. 167: 290- 294 
  10. Mihara, H., H. Sumi, T. Yoneta, H. Mizumoto, R. Ikeda, M. Seiki, and M. Maruyama. 1991. A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus. Jpn. J. Physiol. 41: 461-472 
  11. Kim, W., K. Choi, Y. Kim, H. Park, J. Choi, Y. Lee, H. Oh, I. Kwon, and S. Lee. 1996. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62: 2482- 2488 
  12. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 
  13. Sumi, H., H. Hamada, K. Nakanishi, and H. Hiratani. 1990. Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase. Acta Haematol. 84: 139- 143 
  14. Kim, J. S., I. S. Lee, S. Lee, Y. P. Lee, C. Jung, H. C. Kim, and S. Y. Choi. 2002. Cloning and expression of the aminopeptidase gene from the Bacillus licheniformis in Bacillus subtilis. J. Microbiol. Biotechnol. 12: 773-779 
  15. Kim, T. W., S. H. Jung, J. Y. Lee, S. K. Choi, S. H. Park, J. S. Jo, and H. Y. Kim. 2003. Identification of lactic acid bacteria in Kimchi using SDS-PAGE profiles of whole cell proteins. J. Microbial, Biotechnol. 13: 119- 124 
  16. Hageman, J. H. and B. C. Carlton. 1973. Effects of mutational loss of specific intracellular proteases on the sporulation of Bacillus subtilis. J. Bacteriol. 114: 612- 617 
  17. Chang, C. T., M. H. Fan, F. C. Kuo, and H. Y. Sung. 2000. Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1. J. Agric. Food Chem. 48: 3210- 3216 
  18. Hong, I. P., H. K. lang, S. Y. Lee, and S. G. Choi. 2003. Cloning and characterization of a bifunctional cellulasechitosanase gene from Bacillus licheniformis NBL420. J. Microbiol. Biotechnol. 13: 35- 42 
  19. Kato, T., Y. Yamagata, T. Arai, and E. Ichishima. 1992. Purification of a new extracellular 90-kDa serine protease with isoelectric point of 3.9 from Bacillus subtilis (natto) and elucidation of its distinct mode of action. Biosci. Biotech. Biochem. 56: 1166- 1168 
  20. Kim, H. K., G. T. Kim, D. K. Kim, W. A. Choi, S. H. Park, Y. K. Jeong, and I. S. Kong. 1997. Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish. J. Ferment. Bioeng, 84: 307- 312 
  21. Kim, S. H., N. S. Choi, and W. Y. Lee. 1998. Fibrin zymography: A direct analysis of fibrinolytic enzymes on gels. Anal. Biochem. 263: 115- 116 
  22. Prestidge, L., V. Gage, and J. Spizizen. 1971. Protease activities during the course of sporulation in Bacillus subtilis. J. Bacteriol. 107: 815-823 
  23. Choi, N. S., K. H. Yoo, K. S. Yoon, K. T. Chang, P. J. Maeng, and S. H. Kim. 2005. Identification of recombinant subtilisins. J. Microbiol. Biotechnol. 15: 35-39 
  24. Wu, X. C. S. Nathoo, A. S. H. Pang, T. Carne, and S. L. Wong. 1990. Cloning, genetic organization, and characterization of a structural gene encoding bacillopeptidase F from Bacillus subtilis. J. Biol. Chem. 265: 6845- 6850 
  25. Millet, J. 1970. Characterization of proteinases excreted by Bacillus subtilis Marsburg strain during sporulation. J. Appl. Bacteriol. 23: 207- 219 
  26. Sloma, A., G. A. Jr. Rufo, C. E Rudolph, B. J. Sullivan, K. A. Theriault, and J. Pero. 1990. Bacillopeptidase F of Bacillus subtilis: Purification of the protein and cloning of the gene. J. Bacteriol. 172: 1470- 1477 
  27. Peng, Y., Q. Huang, R. H. Zhang, and Y. Z. Zhang. 2003. Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comp. Biochem. Physiol. 134B: 45- 52 
  28. Kim, S. H. and N. S. Choi. 2000. Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang. Biosci. Biotech. Biochem. 64: 1722- 1725 
  29. Nakamura, T., Y. Yamagata, and E. Ichishima.1992. Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (Natto). Biosci. Biotech. Biochem. 56: 1869- 1871 
  30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning, A Laboratory Manual. Second Ed. CSH, New York, U.S.A. 
  31. Uehara, H., K. Yamane, and B. Maruo, 1979. Therrnosensitive, extracellular neutral protease in Bacillus subtilis: Isolation, characterization and genetics. J. Bacteriol. 139: 583- 590 

이 논문을 인용한 문헌 (16)

  1. 2006. "" Journal of microbiology and biotechnology, 16(11): 1832~1836 
  2. 2006. "" Journal of microbiology and biotechnology, 16(2): 264~271 
  3. 2006. "" Journal of microbiology and biotechnology, 16(3): 368~373 
  4. 2006. "" Journal of microbiology and biotechnology, 16(3): 457~464 
  5. 2007. "" Journal of microbiology and biotechnology, 17(12): 2027~2032 
  6. 2007. "" Journal of microbiology and biotechnology, 17(10): 1688~1694 
  7. 2007. "" Journal of microbiology and biotechnology, 17(9): 1469~1476 
  8. 2007. "" Journal of microbiology and biotechnology, 17(2): 359~363 
  9. 2007. "" Journal of microbiology and biotechnology, 17(5): 761~768 
  10. 2007. "" Journal of microbiology and biotechnology, 17(6): 1018~1023 
  11. 2007. "" Journal of microbiology and biotechnology, 17(8): 1271~1283 
  12. 2008. "" Food science and biotechnology, 17(4): 766~771 
  13. 2010. "" Biotechnology and bioprocess engineering, 15(3): 446~452 
  14. 2010. "" Food science and biotechnology, 19(2): 327~334 
  15. 2010. "" Food science and biotechnology, 19(3): 697~702 
  16. 2011. "" Journal of medicinal food, 14(6): 625~629 

원문보기

원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일