Abstract

End-stage renal disease is a fatal and devastating disease that is caused by progressive and irreversible loss of functioning nephrons in the kidney. Dialysis and renal transplantation are the common treatments at present, but these treatments have severe limitations. The present study investigated the possibility of reconstructing renal tissues by transplantation of renal precursor cells to replace the current treatments for end-stage renal disease. Embryonic renal precursor cells, freshly isolated from metanephroi of rat fetus at day 15 post-gestation, were seeded on biodegradable polymer scaffolds and transplanted into peritoneal cavities of athymic mice for three weeks. Histologic sections stained with hematoxylin & eosin and periodic acid-Schiff revealed the formation of primitive glomeruli, tubules, and blood vessels, suggesting the potential of embryonic renal precursor cells to reconstitute renal tissues. Immunohistochemical staining for proliferating cell nuclear antigen, a marker of proliferating cells, showed intensive nuclear expression in the regenerated renal structures, suggesting renal tissue reconstitution by transplanted embryonic renal precursor cells. This study demonstrates the reconstitution of renal tissue in vivo by transplanting renal precursor cells with biodegradable polymer scaffolds, which could be utilized as a new method for partial or full restoration of renal structure and function in the treatment of end-stage renal disease.

주제어

#Tissue engineering   #renal precursor cell   #biodegradable polymer scaffold  

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참고문헌 (24)

1. Dekel, B., T. Burakova, H. Ben-Hur, H. Marcus, R. Oren, J. Laufer, and Y. Reisner. 1997. Engraftment of human kidney tissue in rat radiation chimera II: Human fetal kidneys display reduced immunogenicity to adoptively transferred human peripheral blood mononuclear cells and exhibit rapid growth and development. Transplantation 64: 1550-1558 
2. Dekel, B., N. Arnariglio, N. Kaminski, A. Schwartz, E. Goshen, F. D. Arditti, I. Tsarfaty, J. H. Passwell, Y. Reisner, and G. Rechavi. 2002. Engraftment and differentiation of human metanephroi into functional mature nephrons after transplantation into mice is accompanied by a profile of gene expression similar to normal human kidney development. J. Am. Soc. Nephrol. 13: 977- 990 
3. Hammerman, M. R. 2002. Transplantation of developing kidneys. Transplant. Rev. 16: 62- 71 
4. Kim, B. S., S. I. Jeong, S. W. Cho, J. Nikolovski, D. J. Mooney, S. H. Lee, O. Jeon, T. W. Kim, S. H. Lim, Y. S. Hong, C. Y Choi, Y. M. Lee, S. H. Kim, and Y. H. Kim. 2003. Tissue engineering of smooth muscle under a mechanically dynamic condition. J. Microbiol. Biotechnol. 13: 841- 845  원문보기 상세보기
5. Koseki, C., D. Herzlinger, and Q. Al-Awqati. 1991. Integration of embryonic nephrogenic cells carrying a reporter gene into functioning nephrons. Am. J. Physiol. 261: C550- C554 
6. Oliver, J. A., J. Barasch, J. Yang, D. Herzlinger, and Q. AlAwqati. 2002. Metanephric mesenchyme contains embryonic renal stem cells. Am. J. Physiol. Renal Physiol. 283: F799-F809 
7. Lanza, R. P, H. Y. Chung, J. J. Yoo, P. J. Wettstein, C. Blackwell, N. Borson, E. Hofmeister, G. Schuch, S. Soker, C. T. Moraes, M. D. West, and A. Atala. 2002. Generation of histocompatible tissues using nuclear transplantation. Nature Biotechnol. 20: 689- 696 
8. Langer, R. and J. P. Vacanti. 1993. Tissue engineering. Science 260: 920- 926 
9. Hammerman, M. R. 2003. Tissue engineering the kidney. Kidney Int. 63: 1195- 1204 
10. Kim, D. I., H. J. Park, H. S. Eo, S. W. Suh, J. H. Hong, M. J. Lee, J. S. Kim, I. S. Jang, and B. S. Kim. 2004. Comparati ve study of seeding and culture methods to vascular smooth muscle cells on biodegradable scaffold. J. Microbiol. Biotechnol. 14: 707-714  원문보기 상세보기
11. Santavirta, S., Y. T. Konttinen, T. Saito, M. Gronblad, E. Partio, P. Kernppinen, and P. Rokkanen. 1990. Immune response to polyglycolic acid implants. J. Bone Joint Surg. Br. 72: 597- 600 
12. Humes, D. H., D. A. Buffington, S. M. MacKay, A. J. Funke, and W. F. Weitzel. 1999. Replacement of renal function in uremic animals with a tissue engineered kidney. Nature Biotechnol. 17: 451- 455 
13. Kim, B. S., D. J. Mooney, and A. Atala. 2000. Genitourinary system, pp. 655-667. In R. P. Lanza, R. Langer, and J. Vacanti (eds.), Principles of Tissue Engineering, Academic Press, San Diego, California, U.S.A. 
14. Rogers, S. A., J. A. Lowell, N. A. Hammerman, and M. R. Hammerman. 1998. Transplantation of developing metanephroi into adult rats. Kidney Int. 54: 27- 37 
15. Kim, S. K., S. H. Yu, J. H. Lee, J. Y Lee, A. Rademacher, D. H. Lee, and J. K. Park. 2001. Effect of collagen concentration on the viability and metabolic function of encapsulated hepatocytes. J. Microbiol. Biotechnol. 11: 423- 427 
16. Lee, D. H., J. H. Lee, J. E. Choi, Y. J. Kim, S. K. Kim, and J. K. Park. 2002. Determination of optimum aggregates of porcine hepatocytes as a cell source of a bioartificialliver. J. Microbiol. Biotechnol. 12: 735- 739  원문보기 상세보기
17. Al-Awqati, Q. and J. A. Oliver. 2002. Stem cells in the kidney. Kidney Int. 61: 387- 395 
18. Hyink, D. P., D. C. Tucker, P. L. St. John, V. Leardkamolkarn, M. A. Accavitti, C. K. Abrass, and D. R. Abrahamson. 1996. Endogenous origin of glomerular endothelial and mesangial cells in grafts of embryonic kidneys. Am. J. Physiol. 270: F886-F889 
19. Kun, N. and K. H. Park. 2004. Immobilization of Arg-GlyAsp (RGD) sequence in sugar-containing copolymer for culturing fibroblast cells. J. Microbiol. Biotechnol. 14: 193-196  원문보기 상세보기
20. MacKay, S. M., A. J. Funke, D. A. Buffington, and H. D. Humes. 1998. Tissue engineering of a bioartificial renal tubule. Am. Soc. Artif. Intern. Organs J. 44: 179- 183 
21. Dekel, B., T. Burakova, F. D. Arditti, S. Reich-Zeliger, O. Milstein, S. Aviel-Ronen, G. Rechavi, N. Friedman, N. Kaminski, J. H. Pass well, and Y. Reisner. 2003. Human and porcine early kidney precursors as a new source for transplantation. Nature Med. 9: 53- 60 
22. Amiel, G. E. and A. Atala. 1999. Current and future modalities for functional renal replacement. Urol. Clin. North Am. 26: 235- 246 
23. Woolf, A. S., S. J. Palmer, M. L. Snow, and L. G. Fine. 1990. Creation of a functioning chimeric mammalian kidney. Kidney Int. 38: 991- 997 
24. Yoo, J. J., T. G. Kwon, and A. Atala. 2002. Intracorporeal kidney, pp. 999- 1003. In A. Atala, and R. P Lanza, (eds.), Methods of Tissue Engineering: Academic Press, San Diego, California, U.S.A. 

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