[논문]CHARACTERISTICS OF BIOHYDROGEN PRODUCTION AND MICROBIAL COMMUNITY AS A FUNCTION OF SUBSTRATE CONCENTRATION

Youn, Jong-Ho; Shin, Hang-Sik

doi:10.4491/eer.2005.10.1.007

CHARACTERISTICS OF BIOHYDROGEN PRODUCTION AND MICROBIAL COMMUNITY AS A FUNCTION OF SUBSTRATE CONCENTRATION 원문보기

Environmental engineering research, v.10 no.1, 2005년, pp.7 - 14

Youn, Jong-Ho , Shin, Hang-Sik

Abstract ▼ AI-Helper

The feasibility of hydrogen production with a raw seed sludge through direct acclimation of feedstock was investigated at acidogenic stage, and methane was harvested at followed methanogenic stage in an anaerobic two-stage process. Hydrogen content was higher than 57% at all tested organic loading rates (OLRs) and the yield of hydrogen ranged from 1.5 to 2.4 mol H2/mol hexose consumed and peaked at 6 gVSl-1day-1. Normal butyrate and acetate were main volatile fatty acids (VFAs), whereas the concentration of propionate was insignificant. The hydrogen-producing bacteria, Clostridium thermosaccharolyticum, was detected with strong intensity at all tested organic loading rates (OLRs) by denaturing gradient gel electrophoresis (DGGE) of the polymerase chain reaction (PCR) analysis. From COD balance in the process, the fraction of the feed-COD converted to the hydrogen-COD at acidogenic stage ranged from 7.9% to 9.3% and peaked at 6 gVSl-1day-1, whereas the fraction of feed-COD converted to the methane-COD at methanogenic stage ranged from 66.2% to 72.3% and peaked at 3 gVSl-1day-1.

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가설 설정

1. It was feasible to obtain H₂-producing bacteria from a raw seed sidge through direct acclimation of feedstock without pretreatment. 2.

제안 방법

In order to identify the responsible hydrogenproducing bacteria and investigate their dynamic behavior in response to OLRs, DNA in the microbial community at each tested OLR was extracted at steady-state by using the Ultraclean DNA Kit (Cat # 12800-50; Mo Bio Laboratory Inc., USA). For the amplication of 16S rDNA fragments, EUB 357f(5'・CCTACGGGAGGCAG CAG-3') and UNIV518r(5'-ATTACCGCGGCTG CTGG-3') with a GC clamp were used as a forward and reverse primer, respectively.
For the amplication of 16S rDNA fragments, EUB 357f(5'・CCTACGGGAGGCAG CAG-3') and UNIV518r(5'-ATTACCGCGGCTG CTGG-3') with a GC clamp were used as a forward and reverse primer, respectively. PCR products were purified using Multi Screen Vacuum Manifold (MILLIPORE com., USA), and search of the GenBank database was conducted using the BLAST program.

대상 데이터

The gas volume was corrected to standard temperature (0℃) and pressure (760mmHg) (STP). Experiments were run at different OLRs, namely 3 gVSleday'1, 6 gVSl'May1 and 8 gVSl'day1. At each OLR, the experiments were carried out for 2 months.

이론/모형

005 M was used as a mobile phase. Carbohydrate was measured using the calorimetric ferric-cyanide method.Measurements of total solid (TS), volatile solid (VS) and pH were performed according to the Standard Methods.

성능/효과

4. The fraction of feed-COD converted to the hydrogen-COD ranged from 7.9% to 9.3% at the acidogenic stage, whereas the fraction of feed-COD converted to the methane-COD ranged from 66.2% to 72.3% at the methanogenic stage.

참고문헌 (20)

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DOI : 10.4491/eer.2005.10.1.007 [무료]
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