[논문]Radical Scavenging Potential of Hydrophilic Phlorotannins of Hizikia fusiformis

Siriwardhana, Nalin; Lee, Ki-Wan; Jeon, You-Jin

doi:10.4490/algae.2005.20.1.069

Radical Scavenging Potential of Hydrophilic Phlorotannins of Hizikia fusiformis 원문보기

Algae, v.20 no.1, 2005년, pp.69 - 75

Siriwardhana, Nalin (Faculty of Applied Marine Science) , Lee, Ki-Wan (Faculty of Applied Marine Science) , Jeon, You-Jin (Faculty of Applied Marine Science)

Abstract ▼ AI-Helper

Hizikia fusiformis is well known edible brown seaweed both in Korea and Japan. It has been intensively studied due to its pronounced health benefits. In this study, the radical scavenging (antioxidative) activities of its hydrophilic phlorotannin constituents were studied. An aqueous extract/original extract (OE) of H. fusiformis was initially prepared with heat, enzymes and pH control treatments. Then the original extract was further fractionated (with methylene chloride and methanol) and crude hydrophilic phlorotannin extract (CHPE) was prepared. The radical scavenging activities of both OE and CHPE were determined by using electron spin resonance (ESR) spectrophotometric assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical assays. The CHPE reported significantly (p < 0.05) higher total phenolic (phlorotannins) content (1.23 mg ${\cdot}ml^{-1}$) than that of the OE (0.21 mg ${\cdot}ml^{-1}$). Both OE and CHPE have reported good radical scavenging activities and those activities were dose-dependent. The CHPE have demonstrated significantly higher radical scavenging activities than that of the OE. In comparison, the DPPH radical (6 $\times$ 10$^{-5}$ M) scavenging activities of all the CHPE concentrations (0.25, 0.5 and 1 mg ${\cdot}ml^{-1}$) tested were significantly higher (37.3, 78.2 and 91.6%, respectively) than that of the OE counterparts (11.4, 34.6 and 61.7%, respectively). Alkyl radical scavenging percentages of CHPE at 0.0625, 0.125, 0.25 and 0.5 mg${\cdot}ml^{-1}$ were significantly higher (34.3, 69.2, 80.4 and 88.7%, respectively) than that of the OE (16.6, 41.4, 62.3 and 77.4%, respectively). The percentages of hydroxyl radical scavenging activities of CHPE at the concentrations of 0.25, 0.5 and 1 mg ${\cdot}ml^{-1}$ were 32.5, 59.4 and 84.2 % respectively. Moreover, the hydroxyl radical scavenging activity of OE was quite lower than that of the CHPE. Therefore, these results suggest that the hydrophilic phlorotannins of H. fusiformis are potential radical scavengers thus, a great source of antioxidative nutraceuticles.

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문제 정의

The objective of the present study was to demonstrate the antioxidant potential of hydrophilic phlorotannis of H. fusiformis by using electron spin resonance (ESR) spectrophotometric assays. Further, to draw the attention of future antioxidative nutraceutical industries, thus to explore the utilization of hydrophilic phlorotannins of H.

데이터처리

5). In the data analysis, variance analyses were performed using Students t test and the differences between means of treatment were determined. P-value of less than 0.

후속연구

fusiformis by using electron spin resonance (ESR) spectrophotometric assays. Further, to draw the attention of future antioxidative nutraceutical industries, thus to explore the utilization of hydrophilic phlorotannins of H. fusiformis as a potential bio-resource in the industry.

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