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Identification of Different Species and Dultivars of Brassica by SDS-PAGE, Isozyme and Molecular Marker

Journal of plant biotechnology, v.7 no.1, 2005년, pp.27 - 35  

Mukhlesur Rahman Md. (Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Department of Genetics and Plant Breeding, Bangladesh Agricultural University) ,  Hirata Yutaka (Graduate School of Agriculture, Tokyo University of Agriculture and Technology)

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Eighty-five different cultivars of Brassica rapa, B. juncea, B. nap us, B. carinata, B. oleracea and hexaploid Brassica collected from Bangladesh, Japan, China and Denmark were analyzed by SDS-PAGE for seed and leaf protein variations, using esterase, acid phosphatase and peroxidase isozyme analysis...

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  • Eighty-five different cultivars of B. rapa, B. juncea, B. na-pus, B. carinata, B. oleracea and hexaploid Brassica, collected from Bangladesh, Japan, China and Denmark were used for this experiment (Table 1).
  • (2000) used a brown seeded cultivar of Indian origin and yellow seeded cultivar from Poland. This study used Japanese and Danish yellow seeded cultivars, and Japanese and Bangladeshi brown seeded cultivars. Therefore, perhaps due to genetic diversity of yellow seeded B.

이론/모형

  • 02% O-dianisidine & 1% H2O2. Acid phosphatase and esterase isozymes extraction, electrophoresis and detections were followed using the methods of Nozaki et al. (1995).
  • The seed coat color specific primers of B.juncea, which was developed by Negi et al, (2000) were used in this experiment. The primers combinations were as follows:
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참고문헌 (19)

  1. Anand IJ, Reddy WR, Rawat DS (1985) Inheritance of seed color in mustard. Indian J Genet 45: 34-37 

  2. Chauhan YS, Kumar K (1987) Genetics of seed color in mustard (Brassica juncea L. Czern and Coss). Cruciferae Newsl 12: 22-23 

  3. Chen BY, Heneen WK, Simonsen V (1989) Comparative and genetic studies of isozymes in resynthesized and cultivated Brassica napus L., B. campestris L. and Brassica alboglabra Bailey. Theor Appl Genet 77: 673-679 

  4. Chevre AM, Delourme R, Eber F, Margale E, Quiros CF, Arus P (1994) Genetic analysis and nomenclature for seven isozyme systems in Brassica nigra, B. oleracea and B. campestris. Plant Breeding 114: 473-480 

  5. Das S, Rajagopal J, Bhatia S, Srivastava PS, Lakshmikumaran M (2000) Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DAN markers. Plant Molecular Biology Division, Tata Energy Research Institute, Lodhi Road, New Delhi, India 

  6. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini preparation: Version II. Plant Mol Biol Rpt 1: 19-21 

  7. Eber F, Chevre AM, Baranger A, Vallee P, Tanguy X, Renard M (1994) Spontaneous hybridization between a male sterile oilseed rape and two weeds. Theor Appl Genet 88: 362-368 

  8. Kumar R, Gupta VP (1985) Isozyme studies in Indian mustard (Brassica juncea L.). Theor Appl Genet. 69: 1-4 

  9. Moriyuki S, Nagamine T, Terauchi T, Akamine F, Sugimoto A (1999) Isozyme application for variety identification and progeny hybridity in Japanese sugercane. Breeding Science 49: 89-95 

  10. Negi MS, Devic M, Delseny M, Lakshmikumaran M (2000) Identification of AFLP fragments linked to seed coat color in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101: 146-152 

  11. Nozaki, T., Anji, M., Takahashi, T. and Ikehashi, H. (1995) Analysis of isozyme loci and their linkage in Brassica campestris L. Breeding Science 45: 57-64 

  12. Person K, Fait AS, von Bothmer R (2001) Genetic diversity of allozymes in turnip (Brassica rapa L. var. rapa) from the Nordic area. Hereditas 134: 43-52 

  13. Quiros CF, Ochoa O, Kianian SF, Douches D (1988) Exploring the role of x 7 species in Brassica evolution: hybridization with B. nigra and B. oleracea. J Heredity 79: 351-358 

  14. Rahman MH (2001) Introgression of alleles of the isozymic locus glucose phosphatase isomerase-2 (GPI-2) from the CC genome of Brassica carinata to the CC genome of Brassica alboglabra and their independent segregation from seed color. Plant Breeding 120: 363-364 

  15. Ripley V, Thrope L, Iler S, Mizier K, Beversdorf WD (1992) Isozyme analysis as a tool for introgression of Sinapis alba germplasm into B. napus. Theor Appl Genet 84: 403-410 

  16. Robbelen G, 1960. Beitrage zur Analyse des Brassica-Ge-noms. Chromosome 11: 205-228 

  17. Takehisa I, Noda S, Mori S, Yamashita M, Nakanishi H, Inoue M, Kamijima O (2001) Genetic variation and geographical distribution of Azuki bean (Vigna angularis) land races based on the electrophoregram of seed storage proteins. Breeding Sciences 51: 225-230 

  18. Tanksley SO, Jones RA (1986) Application of alcohol bedydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort Sci 16: 179-181 

  19. Thanh VC, Hirata Y (2002) Seed storage protein diversity of three rice species in the Mekong Delta. Biosphere Conservation 4: 59-67 

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