Lee Gunho
(Genome Research Institute, Chungnam National University)
,
Yeon Mooshik
(Genome Research Institute, Chungnam National University)
,
Hur Yoonkang
(Genome Research Institute, Chungnam National University)
To clone blue and white flower color specific genes, mRNA differential display was carried out with two different Commelina species, C. communis Linne for blue color and C. coreana Leveille for. leucantha Nakai for white color. Fifty two and 100 cDNA clones specific for blue or white flower color, r...
To clone blue and white flower color specific genes, mRNA differential display was carried out with two different Commelina species, C. communis Linne for blue color and C. coreana Leveille for. leucantha Nakai for white color. Fifty two and 100 cDNA clones specific for blue or white flower color, respectively, were ranging from 200 to 700 bp in size. From the reverse northern blot analysis, 12 and 7 positive clones were selected for blue and white flower, respectively. These clones appear to be novel cDNAs for these Commelina plants, but not color specific. This finding was supported by the northern blot analysis. However, two clones, B18 and B19, derived from blue flowered Commelina were highly expressed than in the white Commelina species, implying that further study will be valuable. The results indicated that both mRNA display experiment and dot blot analysis may not sensitive enough to clone color-determining gene from the plant, leading to explore more advanced method, like high-density colony array study (HDCA).
To clone blue and white flower color specific genes, mRNA differential display was carried out with two different Commelina species, C. communis Linne for blue color and C. coreana Leveille for. leucantha Nakai for white color. Fifty two and 100 cDNA clones specific for blue or white flower color, respectively, were ranging from 200 to 700 bp in size. From the reverse northern blot analysis, 12 and 7 positive clones were selected for blue and white flower, respectively. These clones appear to be novel cDNAs for these Commelina plants, but not color specific. This finding was supported by the northern blot analysis. However, two clones, B18 and B19, derived from blue flowered Commelina were highly expressed than in the white Commelina species, implying that further study will be valuable. The results indicated that both mRNA display experiment and dot blot analysis may not sensitive enough to clone color-determining gene from the plant, leading to explore more advanced method, like high-density colony array study (HDCA).
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제안 방법
However, none of these possesses bright blue color as much as that of Commelina communis. This study was undertaken to iso late the bhje color determination genes by differential dis play reverse transcription PCR (DDRT-PCR) using mRNA populations from different-colored, blue and white flowered, Commelina plants.
대상 데이터
The recombinant plasmid DNA was se quenced using the ABI Prism 3700 automated sequencer (Applied Biosystem Division of Perkin Elmer). Sequence data were analyzed with known secpences in the National Center for Biotechnology Information database (www.ncbi. nlm.nih.gov) with BLAST search program.
Two Commelina species, blue flowered (C. communis Li- nne) and white flowered (C. coreana Leveille for. leucantha Nakai), were collected from the field and grown at the campus of the Chungnam National University, Korea. Floral buds and flowers (Fig.
성능/효과
These DNA bands were eluted and am plified by PCR. Finally, 52 and 100 cDNA clones specific for bhje or white flowered Commelina species, respectively, were obtained (Figures 2, 3). These bands ranged from 200 to 700 bp in size.
참고문헌 (21)
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