[논문]Effect of Nutrient Media and Sucrose Concentration on Shoot Organogenesis in Tomato

Bhatia Poonam

Effect of Nutrient Media and Sucrose Concentration on Shoot Organogenesis in Tomato 원문보기

Journal of plant biotechnology, v.7 no.1, 2005년, pp.57 - 65

Bhatia Poonam (Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Institute of Molecular and Cellular Biosciences, The University of Tokyo)

Abstract ▼ AI-Helper

The $F_1$ hybrid Red Coat is one of the most highly sought after cultivars of tomato in Australia and yields up to 7.5 $\cal{kg/plant}$. An experiment was conducted to de-termine the optimal strength and type of growth medium and sucrose concentration for shoot organogenesis of the Red Coat cultivar using cotyledonary explants. Two basal growth media, viz. MS and Gamborg' s $B_5$ at 0, 1/4, 1/2, full or double strength along with sucrose concentrations of 0, 0.5, 1.5, 3 or $5\%$, were evaluated using 25 replications. The main effects of treatment and their mutual interactions were evaluated for the proportion of explants that produced callus and/or shoots, number of shoots produced per explant, callus diameter and shoot height. The explants failed to produce shoots in the absence of mineral nutrient. Only a small proportion of the explants ($6\%$ with $B_5\;and\;3\%$ with MS) regenerated shoots in the absence of sucrose. Lower sucrose concentrations ($0.5-1.5\%$) along with full strength media were optimal for most of the traits studied. The $B_5$ medium outperformed MS medium for shoot organogenesis. For all the traits examined, significant differences in main effects (P < 0.05) and two-way interactions were detected, but no three-way interactions (medium type $\times$ medium concentration $\times$ sucrose concentration) were observed. Sucrose was found essential for the development of chlorophyll. Chlorophyll content increased with an increase in sucrose concentration up to $3\%$ and decreased at $5\%$ sucrose.

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제안 방법

In an effort to develop a cost-effective protocol for mass propagation of the Red Coat cutivar, different strengths (0, 1/4%, 1/2%, full or double strength) of two basal growth media, MS (Murashige and Skoog 1962) and Gamborg's B₅ (Gamborg et al. 1968) and varying concentrations of sucrose (0, 0.5, 1.5, 3 or 5%) were evaluated and organogenic and callus responses of cotyledon explants were quantified.
Observations were recorded after four weeks of inoculation for fequency of explants showing organogenesis, callus formation, callus diameter, number of shoots produced per explant and shoot height.
The treatments consisted of all possible combinations of the two types of media, five media strengths and five sugar concentrations. Twenty-five culture tubes were used per treatment combination and the tubes were incubated in a controlled environment room according to a completely ran domised design.

이론/모형

For num ber of shoots produced per explant, shoot height and callus diameter, only those cultures in which shoot or callus was produced were considered in the analysis. As such, these data were unbalanced and were analysed in GenStat using the procedure AUNBALANCED (Payne 2002). Pair-wise comparison of means was performed using the least sig nificant difference (LSD) test at the 5% level.
For the percentage of regeneration and callus response, only the treatments that showed some regeneration were included in the analysis. Data were analysed using GenStat® statistical software with a generalised linear model (GLM; McCullagh and Nelder 1983) assuming a binomial error and a logit link function (for shoot regeneration and callus response). The initial model included the effects of media, media concent ration, sucrose concentration and the corresponding two- way and three-way interactions.
The means presented are those predicted from the final model. For shoot regeneration and callus response, pair-wise comparisons of means were conducted on the logit scale as per the GenStat RPAIR procedure.

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