[논문]Rapid in vitro Germination of Zygotic Embryos via Endosperm Removal in Eleutherococcus senticosus

You Xiang-Ling; Choi Yong-Eui; Yi Jae-Seon

Rapid in vitro Germination of Zygotic Embryos via Endosperm Removal in Eleutherococcus senticosus 원문보기

Journal of plant biotechnology, v.7 no.1, 2005년, pp.75 - 80

You Xiang-Ling (Division of Forest Resources, College of Forest Sciences, Kangwon National University) , Choi Yong-Eui (Division of Forest Resources, College of Forest Sciences, Kangwon National University) , Yi Jae-Seon (Division of Forest Resources, College of Forest Sciences, Kangwon National University)

Abstract ▼ AI-Helper

Eleutherococcus senticosus (also called Acanthopanax senticosus), belonging to Araliaceae family, has been used as an important medicinal woody plant. Mature seeds of Eleutherococcus senticosus have rudimentary (extremely immature) zygotic embryos and require a long-term stratification for about 18 months to induce germination. Here, through the methods of endosperm removal and other exogenous treatments, we investigated the factors for inducing rudimentary embryos by in vitro culture, Rudimentary zygotic embryos in seeds were at globular to heart-shaped stage at about $250{\mu}m$ in length just after harvest of fruits. When the seeds without testa were cultured on 1/2 MS (Murashige and Skoog 1962) medium, they did not germinate regardless of medium and sucrose concentrations but the removal of endosperm tissue markedly stimulated the growth of rudimentary zygotic embryos. The embryo reached ear-lier maturation, once when the endosperm surrounding the rudimentary embryos was removed. Rudimentary zygotic embryos developed cotyledons within 3 weeks of culture after endosperm emoval. However, post-mature zygotic embryos failed to germinate though they were morphologically normal, indicating another dormancy of embryos. $GA_3\;(2.0\;\cal{mg/L})$ and/or charcoal ($0.2\%$) treatment rapidly enhanced the germination of zygotic embryos. These results suggest that E. senticosus seeds have double dormancy; i. e. morphological rudimentary dormancy influenced by surrounding endosperm and physiological dormancy after post-maturation of zygotic embryos. Based on the above findings, we established the rapid germination of rudimentary zygotic embryos by in vitro culture of excised seeds with endosperm removal and $GA_3$ treatment.

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제안 방법

To optimize the growth of zygotic embryos by modifi cation of culture condition, excised seeds with 3/4 of their endosperm removed were cultured on 1/4, 1/2, 1 and 2 MS medium with 1.0% sucrose, or 1/2 MS medium with 0%,1.0%, 3.0% and 5.0% sucrose for 40 days. Growth of zygotic embryos was infkjenced by the strength of MS salts and concentrations of sucrose (Figs.

대상 데이터

E. senticosus Maxim, seeds were collected from the Nursery, College of Forest Sciences, Kangwon National University. They were stratified in moist sand and kept at 15℃for 4 weeks.

데이터처리

using white florescent tubes. All results were statistically determined, representing mean ±SE.

참고문헌 (18)

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상세보기
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