• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


Eleutherococcus senticosus (also called Acanthopanax senticosus), belonging to Araliaceae family, has been used as an important medicinal woody plant. Mature seeds of Eleutherococcus senticosus have rudimentary (extremely immature) zygotic embryos and require a long-term stratification for about 18 months to induce germination. Here, through the methods of endosperm removal and other exogenous treatments, we investigated the factors for inducing rudimentary embryos by in vitro culture, Rudimentary zygotic embryos in seeds were at globular to heart-shaped stage at about $250{\mu}m$ in length just after harvest of fruits. When the seeds without testa were cultured on 1/2 MS (Murashige and Skoog 1962) medium, they did not germinate regardless of medium and sucrose concentrations but the removal of endosperm tissue markedly stimulated the growth of rudimentary zygotic embryos. The embryo reached ear-lier maturation, once when the endosperm surrounding the rudimentary embryos was removed. Rudimentary zygotic embryos developed cotyledons within 3 weeks of culture after endosperm emoval. However, post-mature zygotic embryos failed to germinate though they were morphologically normal, indicating another dormancy of embryos. $GA_3\;(2.0\;\cal{mg/L})$ and/or charcoal ($0.2\%$) treatment rapidly enhanced the germination of zygotic embryos. These results suggest that E. senticosus seeds have double dormancy; i. e. morphological rudimentary dormancy influenced by surrounding endosperm and physiological dormancy after post-maturation of zygotic embryos. Based on the above findings, we established the rapid germination of rudimentary zygotic embryos by in vitro culture of excised seeds with endosperm removal and $GA_3$ treatment.

참고문헌 (18)

  1. Brekham II (1960) A new medicinal plant of the family Araliaceae-the spiny ELetttherococcus. Izv Sibir Otdel Akad Nauk USSR 9: 113-120 
  2. Choi YE, Kim JW, Yoon ES (1999) High frequency of plant production via somatic embryogenesis from callus or cell suspension cultures in Eleutherococcus senticosus. Ann Bot 83: 309-314 
  3. Hepher A, Roberts JA (1985a) The control of seed germination in Trollius Ledebouri: The breaking of dormancy. Planta 166: 314-320 
  4. Liu LD, Wang ZL, Tian GW and Shen JH (1998) The development of embryo and endosperm in Eletherococcus senticosus (Araliaceae). Acta Phytotaxonomica Sinica 36: 298-304 
  5. Park HK, Park MS, Kim TS, Kim S, Choi KG, Park KH (1997) Characteristics of embryo growth and dehiscence during the after-ripening period in Eleutherococcus senticosus. Korean J Crop Sci 42: 637-677 
  6. Wang PJ and Huang LC (1976) Beneficial effects of activated charcoal on plant tissue and organ culture. In Vitro 12: 260-262 
  7. Zhu N, Wang YH (1992) Reproductive ecological studies of Acanthopanax senticosus (II)---Seed dispersal, seed bank and recruitment. Journal of Northeast Forestry University 20: 13-17 
  8. Hu CY (1976) Light-mediated inhibition of in vitro development of rudimentary embryos of llex opaca. Amer J Bot 63: 651-656 
  9. Hepher A, Roberts JA (1985b) The control of seed germination in Trollius Ledebouri: The model of dormancy. Planta 166: 321-328 
  10. Hu CY (1975) In vitro culture of rudimentary embryos of eleven llex species. J Amer Soc Horti Sci 100: 221-225 
  11. Hudson T, Hartmann, Dale E, Kester, Fred E. Davies (1990) Plant propagation-principles and practices. Prentice Hall Career and Technology Englewood Cliffs, New Jersey pp 114 
  12. Fridborg G, Pedersen M, Landsrom, Eriksson T (1978) The effect of activated charcoal on tissue cultures: Absorption of metabolites inhibiting morphogenesis. Physiol Plant 43: 104-106 
  13. Linsmaier EM and Skoog F (1983). Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100-127 
  14. Sansberro PA, Rey HY, Mrogiski LA and Collavino MM (1998) In Vitro culture of rudimentary embryos of llex paraguariensis: responses to exogenous cytokinins. J Plant Growth Regul 17: 101-105 
  15. Zhao Y, Liu H, Liu T, Fu J, Liu W, Zhang X (2000) Affection of exogenous gibberellic acid ($GA_3$) on endogenous hormones of Panax quinquefolium seed during its morphological after-ripening period. Zhong Yao Cai 23: 593 
  16. Bewley JD (1997) Seed germination and dormancy. Plant Cell 9: 1055-1066 
  17. Isoda S, Shoji J (1994) Studies on the cultivation of Eleutherococcus senticosus Maxim. II On the germination and raising of seedling. Natural Medicine 48: 75-81 
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-479 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일