영지버섯(Ganoderma lucidum)으로부터 영양 및 기능성 성분의 열수추출 수율을 증대시킬 수 있는 방법을 개발하기 위하여 추출 전처리 및 방법의 효과를 비교, 검토하였다. 영지를 분쇄하여 사별하고 초음파와 마이크로파 처리를 10분 동안 실시한 후 물을 용매로 상압 및 가압추출을 각각 2시간 동안 실시하였다. 추출전 전처리를 행함으로서 총당, 단백질함량 및 페놀성물질의 추출수율을 증가시켰고, 유리기 소거능이 증가하는 경향을 보였으며 마이크로파 전처리가 초음파처리 보다 우수한 것으로 나타났다. 또한 상압추출보다 가압추출에 의해 가용성 고형물, 총당, 단백질 및 페놀성 물질의 추출수율이 증대되는 경향을 보였다. 따라서 초음파나 마이크로파 전처리와 가압추출은 영지버섯의 추출효율 향상에 유효한 방법인 것으로 판단되었다.
영지버섯(Ganoderma lucidum)으로부터 영양 및 기능성 성분의 열수추출 수율을 증대시킬 수 있는 방법을 개발하기 위하여 추출 전처리 및 방법의 효과를 비교, 검토하였다. 영지를 분쇄하여 사별하고 초음파와 마이크로파 처리를 10분 동안 실시한 후 물을 용매로 상압 및 가압추출을 각각 2시간 동안 실시하였다. 추출전 전처리를 행함으로서 총당, 단백질함량 및 페놀성물질의 추출수율을 증가시켰고, 유리기 소거능이 증가하는 경향을 보였으며 마이크로파 전처리가 초음파처리 보다 우수한 것으로 나타났다. 또한 상압추출보다 가압추출에 의해 가용성 고형물, 총당, 단백질 및 페놀성 물질의 추출수율이 증대되는 경향을 보였다. 따라서 초음파나 마이크로파 전처리와 가압추출은 영지버섯의 추출효율 향상에 유효한 방법인 것으로 판단되었다.
This study was conducted to investigate the effects of pretreatment and extraction methods on the water extraction yields of sugar, protein and phenolic compounds, and the antioxidative activity of extract from Ganoderma lucidum. The herb was ground, sifted, and treated for 10 min with microwave (2,...
This study was conducted to investigate the effects of pretreatment and extraction methods on the water extraction yields of sugar, protein and phenolic compounds, and the antioxidative activity of extract from Ganoderma lucidum. The herb was ground, sifted, and treated for 10 min with microwave (2,450 MHz) and ultrasound (20 kHz), and then extracted by boiling underatmospheric pressure and pressured boiling. Particle size $(0.8\~2.4\;mm)$, extraction time (2 hr) and solvent/solid ratio (20:1) were selected as optimal conditions for extraction.. Microwave and ultrasound treatment increased the extraction yields of total sugar, protein and phenolic compounds and the radical scavenging activity of extract. In comparison with microwave treatment and ultrasound treatment, microwave treatment was more effective than that of ultrasound treatment. The yields of all three compounds and the radical scavenging activity of extract were higher in pressured boiling extraction than in boiling underatmospheric pressure. The results showed that microwave radiation and sonication prior to extraction, and pressured extraction could be utilized for improving the extraction efficiency of G. lucidum.
This study was conducted to investigate the effects of pretreatment and extraction methods on the water extraction yields of sugar, protein and phenolic compounds, and the antioxidative activity of extract from Ganoderma lucidum. The herb was ground, sifted, and treated for 10 min with microwave (2,450 MHz) and ultrasound (20 kHz), and then extracted by boiling underatmospheric pressure and pressured boiling. Particle size $(0.8\~2.4\;mm)$, extraction time (2 hr) and solvent/solid ratio (20:1) were selected as optimal conditions for extraction.. Microwave and ultrasound treatment increased the extraction yields of total sugar, protein and phenolic compounds and the radical scavenging activity of extract. In comparison with microwave treatment and ultrasound treatment, microwave treatment was more effective than that of ultrasound treatment. The yields of all three compounds and the radical scavenging activity of extract were higher in pressured boiling extraction than in boiling underatmospheric pressure. The results showed that microwave radiation and sonication prior to extraction, and pressured extraction could be utilized for improving the extraction efficiency of G. lucidum.
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가설 설정
lucidumwith with different pretreatment and extraction pressure is compared in Fig. 3. The yield showed significant differences according to extraction conditions. In the G lucidum, the yield was increased by pressured extraction regardless pretreatment methods.
제안 방법
But, there are no report about the effects of both microwave and ultrasound treatment prior to the conventional extraction on extraction property. This study compared with the effects of both microwave and ultrasound pretreatment in relation to extraction pressure applied to the extractions of G. lucidum in order to determine preferable treatment method.
대상 데이터
Ganoderma lucidum was obtained from the herb market in Daegu, Korea. The samples were kept in a sealed plastic bag and stored at 4℃ until use.
데이터처리
, USA) for replicated test data. Analyses of variance were performed by an ANOVA procedure. Significant differences(α=0.
Analyses of variance were performed by an ANOVA procedure. Significant differences(α=0.05) between means were determined by Duncan's multiple range test.
이론/모형
Content of total phenolic compounds was determined using the Folin-Ciocalteu method (14). The extract(5mL) was transferred into volumetric flask.
Total sugar content was determined using the phenolsulfuric acid method(12). The extract(1mL) was added to a 1mL of 5% phenol solution plus 5mL of cone sulfuric acid.
성능/효과
lucidum in relation to extraction conditions is presented in Fig. 4. The yield was increased by pressured extraction when same pretreatment was used, enhanced by the treatment of microwave or ultrasound prior to both nonpressured and pressxired extractions. Between the two pretreatments, microwave was more effective on the isolation of phenolic compounds from the herbs.
In general, it was known that radical scavenging activity is positively related to the level of phenolic compounds(3). Result of this study could be explained because phenolic compounds from G. lucidum have a strong radical scavenging activity. Extraction condition affect the radical scavenging activity of the extract of G.
The study showed that the both microwave and ultrasomd treatments and the pressured extraction had increasing effect on the extraction efficiency and the antioxidative ability of extract. Therefore, microwave radiation, ultrasonication and pressured extraction could be recommended as a suitable method to isolate chemical components from G.
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