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논문 상세정보


Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline $PRO^{\circledR}$, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase ad-sorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

참고문헌 (25)

  1. Chase, H. A. (1994) Purification of proteins by adsorption chromatography in expanded beds. Trends Biotechnol. 12: 296-303 
  2. Anspach, F. B., D. Curbelo, R. Hartmann, G. Garke, and W.-D. Deckwer (1999) Expanded-bed chromatography in primary protein purification. J. Chromatogr. A 865: 129-144 
  3. Hamilton, G. E., F. Luechau, S. C. Burton, and A. Lyddiatt (2000) Development of a mixed mode adsorption process for the direct product sequestration of an extracellular protease from microbial batch cultures. J. Biotechnol. 79: 103-115 
  4. Hu, S., L.-H Mei, and S.-J. Yao (2003) Optimization of submerged fermentation of nattokinase production by Bacillus subtilis with response surface methodology. Food Ferment. Ind. (China) 29: 13-17 
  5. Feuser, J., J. Walter, M.-R. Kula, and J. Thommes (1999) Cell-adsorbent interactions in expanded bed adsorption of proteins. Bioseparation 8: 99-109 
  6. Sumi, H., H. Hamada, K. Nakanishi, and H. Hiratani (1990) Enhancement of the fibrinolytic-activity in plasma by oral-administration of nattokinase. Acta Haematol. 84: 139-143 
  7. Hu, H.-B. (1999) Adsorption Process in Expanded Bed: Purification of Anti-thrombus enzyme. Ph.D. Thesis. Zhejiang University, Hangzhou, China 
  8. Technical notes. FastLine$^{\circledR}$ Columns & adsorbents for expanded bed adsorption. UpFront Chromatography A/S: Denmark. http://www.upfront-dk.com 
  9. Huang, J., L.-H Mei, S. Hu, and S.-J. Yao (2003) Optimization of nattokinase fermentation and effect of fed-batch culture on the production of nattokinase. J. Chem. Eng. Chinese Univ. 17(Supplement): 63-66 
  10. Lin, D.-Q., S.-J. Yao, L.-H. Mei, and Z.-Q. Zhu (2004) Parallel strategy in process design of expanded bed adsorption. Chem. Ind. Eng. (China) 23: 33-37 
  11. Burton, S. C. and D. R. K. Harding (1998) Hydrophobic charge induction chromatography: Salt independent protein adsorption and facile elution with aqueous buffers. J. Chromatogr. A 814: 71-81 
  12. Asturp, T. and S. Mullertz (1952) The fibrin plate method for the estimating fibrinolytic activity. Arch. Biochem. Biophys. 40: 346-356 
  13. Hjorth, R. (2002) Design of expanded bed adsorbents towards perfection. Proceedings of 4th International Conference on Expanded Bed Adsorption. September 8-11. Petersburg, USA 
  14. Hjorth, R., P. Leijon, A. K. Barnfield-Frej, and C. Jägersten (1998) Expanded bed adsorption. pp. 199-225. In: G. Subramanian (ed.). Bioseparation and Bioprocessing. Weinheim, Wiley-VCH, NY, USA 
  15. Thommes, J. (1997) Fluidized bed adsorption as a primary recovery step in protein purification. Adv. Biochem. Eng. 58: 185-230 
  16. Zhong, L.-N., D.-Q. Lin, M.-H. Lu, and S.-J. Yao (2004) Quantitative evaluation of biomass/adsorbent interactions in expanded bed: Biomass pulse response method. J. Chem. Ind. Eng. (China) 55: 1908-1911 
  17. Hu, S. (2003) Fundamental Study of a Novel Fibrinolytic Enzyme: Nattokinase. M.D. Thesis. Zhejiang University, Hangzhou, China 
  18. Burton, S. C., N. W. Haggarty, and D. R. K. Harding (1997) One step purification of chymosin by mixed mode chromatography. Biotechnol. Bioeng. 56: 45-55 
  19. Sumi, H., H. Hamada, H. Tsushima, H. Mihara, and H. Muraki (1987) A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese natto: Atypical and popular soybean food in the Japanese diet. Experientia 43: 1110-1111 
  20. Fujita, M., K. Hong, Y. Ito, S. Misawa, N. Takeuchi, K. Kariya, and S. Nishimuro (1995) Transport of nattokinase across the rat intestinal tract. Biol. Pharm. Bull. 18: 1194-1196 
  21. Burton, S. C. and D. R. K. Harding (1997) High-density ligand attachment to brominated allyl matrices and application to mixed mode chromatography of chymosin. J. Chromatogr. A 775: 39-50 
  22. Fujita, M., K. Hong, Y. Ito, R. Fujii, K. Kariya, and S. Nishimuro (1995) Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat. Biol. Pharm. Bull. 18: 1387-1391 
  23. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254 
  24. Lei, Y.-L. (2003) Preparation of Spherical Cellulose/$TiO_2$ Composite Adsorbent for Expanded Bed Adsorption and Its Application to Purification of Proteins. Ph.D. Thesis. Zhejiang University, Hangzhou, China 
  25. Lin, D.-Q., H. M. Fernandez-Lahore, M.-R. Kula, and J. Thommes (2001) Minimising biomass/adsorbent interactions in expanded bed adsorption processes: A methodological methodological design approach. Bioseparation 10: 7-19 

이 논문을 인용한 문헌 (1)

  1. 2007. "" Biotechnology and bioprocess engineering, 12(3): 265~270 


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