[논문]Enhancement of Pyruvate Production by Torulopsis glabrata : through Supplement of Oxaloacetate as Carbon Source

Liu Li-Ming; Du Guo-Cheng; Li Vin; Li Hua-Zhong; Chen Jian

doi:10.1007/bf02932583

Enhancement of Pyruvate Production by Torulopsis glabrata : through Supplement of Oxaloacetate as Carbon Source 원문보기

Biotechnology and bioprocess engineering : Bbe, v.10 no.2, 2005년, pp.136 - 141

Liu Li-Ming (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University) , Du Guo-Cheng (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University) , Li Vin (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University) , Li Hua-Zhong (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University) , Chen Jian (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University)

Abstract ▼ AI-Helper

The capability of utilizing a TCA cycle intermediates as the sole carbon source by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019 was demonstrated with plate count method. It is indicated that T. glabrata could grew on a medium with one of the TCA cycle intermediates as the sole carbon source, but more colonies were observed when glucose, acetate and one of the TCA cycle intermediates coexisted in the medium. Among the intermediates of the TCA cycle examined in this study, cell growth was improved by supplementing oxaloacetate. Further investigation showed that the presence of acetate was necessary when oxaloacetate was supplemented. By supplementing with 10 g/L of oxaloacetate in pyruvate batch fermentation, dry cell weight increased from 11.8 g/L to 13.6 g/L, and pyruvate productivity was enhanced from $0.96\;gL^{-1}h^{-1}\;to\;1.19 gL^{-1}h^{-1}$ after cultivation of 56 h. The yield of pyruvate to glucose was also improved from 0.63 g/g to 0.66 g/g. These results indicate that under vitamins limitation, the productivity and yield of pyruvate could be enhanced via an increase of cell growth by the supplementation of oxaloacetate.

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문제 정의

2). This study was performed to investigate the influence of TCA cycle intermediates on cell growth and pyruvate production by T. glabrata.

대상 데이터

A multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019 which could use NH₄C₁ as a sole nitrogen source and acetate as supplemental carbon source [2] was used in this study.

이론/모형

Pyruvate concentration was determined by enzymatic assay method described by LamprechtW and Heinz F [17]. Glucose concentration was measured with the 3, 5-dinitrylsalicylic acid spectrometric method [18]. Cell growth (dry cell weight, DCW) was monitored by measuring the optical density (OD) at 660 nm after an appropriate dilution.

성능/효과

6). All of these results indicate that the enhancement of oxaloacetate on cell growth of T. glabrata was based on the fact that the strain could convert acetate into cytosolic acetyl-CoA by the PDH complex bypass pathway.
However, with glucose and acetate as carbon sources, 36 ± 2 colonies were obtained. It was interesting to find that all four examined TCA cycle intermediates including malate, succinate, a-ketoglutarate and oxaloacetate could be used as energy and carbon sources for the growth of T. glabrata. Moreover, more colonies were observed when glucose, acetate and one of the TCA cycle intermediates were used as co-substrates.
4b and c). These results indicate that the addition of TCA cycle intermediates can strengthen glucose metabolism, moreover cell growth and pyruvate production, The yields of dry cell weight to glucose consumed (») with glucose and one of the TCA cycle intermediates as carbon sources were 8% to 43.4% higher than that on glucose medium, while the yields of pyruvate to glucose were increased by 8.7% to 17.4% (Table 1).
This research has proven that strain Tbrulopsis glabrata CCTCC M202019 could utilize the TCA cycle intermediates as carbon and energy source for growth. Of these TCA cycle intermediates, oxaloacetate was propitious to cell growth and pyruvate production.

참고문헌 (21)

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DOI : 10.1007/BF02932583 [무료]
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