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논문 상세정보

Abstract

Objectives : DNA methylation is one of the best characterized epigenetic mechanisms that play a regulatory role in genome programming and imprinting during embryogenesis. In this present study, we investigated the association between DNA methylation in the human placenta and the maternal folate and homocysteine concentrations on the Methylenetetrahydrofolatereductase (MTHFR) genetic polymorphism during pregnancy. Methods : We investigated 107 pregnant women who visited Ewha Woman's University Hospital for prenatal care during their $24{\sim}28$ weeks-period of gestation. During the second trimester, we measured the serum homocysteine and folate concentrations . The MTHFR 677 genetic polymorphism was determine by performing PCR-RFLP assay. The expression of DNA methylation in the human placentas was estimated by using immunohistochemistry method. Results : Serum folate was negatively correlated with the serum homocysteine concentration for all the MTHFR genotypes. We found positive correlation between the folate concentrations and the DNA methylation in the human placenta (p<0.05). An increasing concentration of homocysteine was associated with reduced DNA methylation in the human placenta. The coefficient value was -2.03 (-3.77, -0.29) on the regression model (p<0.05). Conclusion : These findings suggest that the maternal folate and homocysteine levels along with the MTHFR 677 genetic polymorphism during pregnancy affect the DNA methylation in the human placenta.

참고문헌 (33)

  1. Kim KN, Kim YJ, Chang NS. The interaction of the 5,10-Methylenetetrahydrofolate reductase polymorphism with folate and vitamin B12 Intakes and serum homocysteine concentrations in pregnant women. Korean Nutr Soc 2002; 35(10): 1045-1052 (Korean) 
  2. Jacob RA, Gretz DM, Taylor PC, James SJ, Pogribny IP, Miller BJ, Henning SM, Swendseid ME. Moderate folate depletion increases plasma homocysteine and decreases lymphocyte DNA methylation in postmenopau sal women. J Nutr 1998; 128: 1204-1212 
  3. Yao X, Hu JF, Daniels M, Shiran H, Zhou X, Yan H, Lu H, Zeng Z, Wang Q, Li T, Hoffman AR. A methylated oligonucleotide inhibits IGF2 expression and enhances survival in a model of hepatocellular carcinoma. J Clin Investig 2003; 111: 265-273 
  4. Laukkanen MO, Mannermaa S, Hiltunen MO, Aittomaki S, Airenne K, Janne J, Yla- Herttuala S. Local hypomethylation in atherosclerosis found in rabbit ec-sod gene. Arterioscler Thromb Vasc Biol 1999: 19: 2171- 2178 
  5. MacLennan NK, James SJ, Melnyk S, Piroozi A, Jernigan S, Hsu JL, Janke SM, Pham TD, Lane RH. Uteroplacental insufficiency alters DNA methylation, one-carbon metabolism, and histone acetylation in IUGR rats. Physiol Genomics 2004; 18(1): 43-50 
  6. Urnov FD. Methylation and the genome. the power of a small amendment. J Nutr 2002; 132: 2450s-2456S 
  7. Miller JW, Nadeau MR, Smith J, Smith D, Selhub J. Folate deficiency induced homocysteinaemia in rats: disruption of Sadenosylmethionine= s co-ordinate regulation of homocysteine metabolism. Biochem J 1994; 298: 415-419 
  8. Castro R, Rivera I, Struys EA, Jansen EW, Ravasco P, Camilo ME, Blom HJ, Jakobs C, Tavares de Almeida I. Increased homocysteine and S-adenosylhomocysteine concentrations and DNA hypomethylation in vascular disease. Clin Chem 2003; 49: 1292-1296 
  9. De Cabo SF, Santos J, Fernandez-Piqueras J. Molecular and cytological evidence of Sadenosyl- L-homocysteine as an innocuous undermethylating agent in vivo. Cytogenet Cell Genet 1995; 71: 187-192 
  10. Wolffe Ap, Matzke Ma. Epigenetics: regulation through repression. Science 1999; 286: 481-486 
  11. Coen VG, Coen DA. Hyperhomocysteinaemia and vascular disease: a role for DNA hypomethylation? Lancet 2003; 361: 17 
  12. Robertson KD, Wolffe AP. DNA methylation in health and disease. Nat Rev Genet 2000; 1:11-19 
  13. Wang H, Yoshizumi M, Lai K, Tsai JC, Perrella MA, Haber E, Lee ME. Inhibition of growth and p21ras methylation in vascular endothelial cells by homocysteine but not cysteine. J Biol Chem 1997; 272: 25380-25385 
  14. Powers RW, Minich LA, Lykins DL, Ness RB. Crombleholme Wr, Roberts JM. Methylenetetrahydrofolate reductase polymorp hism, folate, and susceptibility to preeclampsia. J Gynecol Invest 1999; 6: 74-79 
  15. Araki A, Sako Y. Determination of free and total homocysteine in human plasma by high performance liquid chromatography with fluorescence detection. J Chromatogr 1987; 422: 43-52 
  16. Chandrika JP, Gary LJ, Andra RF, Martin AW, Upendr M, William EG, Douglas CH, Alain N. Immunohistochemical evaluation of global DNA methylation. Biotech Histochem 2000; 75(6): 251-258 
  17. Trasler J, Deng L, MeInyk S, Pogribny I, Hiou- Tim F, Sibani S, Oakes C, Li E, James SJ, Rozen R. Impact of Dnmt1 deficiency, with and without low folate diets, on tumor numbers and DNA methylation in Min mice. Carcinogenesis 2003; 24: 39-45 
  18. Faraci FM, Lentz SR. Hyperhomocysteinemia, oxidative stress, and cerebral vascular dysfunction. Stroke 2004; 35: 345-347 
  19. Slattery ML, Potter JD, Samowitz W, Schaffer D, Leppert M. Methylenetetrahydrofolate reductase, diet and risk of colon cancer. Cancer Epidemiol Biomarkers Prev 1999; 8: 513-518 
  20. Fang JY, Xiao SD. Folic acid, polymorphism of methyl-group metabolism genes, and DNA methylation in relation to GI carcinogenesis. J Gastroenterol 2003; 38: 821-829 
  21. Kim KN, Kim YG, Chang NS. Effects of the interaction between the C677T 5,10- methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women. Eur J Clin Nutr 2004; 58: 10-16 
  22. Zaina S, Lindholm MW, Lund G. Nutrition and aberrant DNA methylation patterns in atherosclerosis: more than just hyperhomocysteinemia? J Nutr 2005; 135(1): 5-8 
  23. Esteller, M, Herman, JG. Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours. J Pathol 2002; 196: 1-7 
  24. Dong C, Yoon W, Goldschmidt-Clermont PJ. DNA methylation and atherosclerosis. J Nutr 2002; 132: 2406S-2409S 
  25. Hiltunen MO. Turunen MP, Hakkinen TP, Rutanen J, Hedman M, Makinen K, Turunen AM, Aalto-Setala K, Yla-Herttuala S. DNA hypomethylation and methyltransferase expression in atherosclerotic lesions. Vasc Med 2002; 7: 5-11. 
  26. Newman PE. Can reduced folic acid and vitamin B12 levels cause deficient DNA methylation producing mutations which initiate atherosclerosis? Med Hypotheses 1999; 53(5): 421-424 
  27. Lee BE, Hong YC, Park HS, Lee JT, Kim JY, Kim YJ, Kim SH, Goo JO, Ha EH. Maternal exposure to environmental tobacco smoke(ETS) and pregnancy outcome(low birth weight or preterm baby) in prospective cohort study. Korean J Prev Med 2003: 36(2): 117- 124 (Korean) 
  28. Chen Z, Karaplis AC, Ackerman SL, Pogribny IP, Melnyk S, Lussier-Cacan S, Chen MF, Pai A, John SW, Smith RS, Bottiglieri T, Bagley P, Selhub J, Rudnicki MA, James SJ, Rozen R. Mice deficient in methylenetetrahydrofolate reductase exhibit hyperhomocysteinemia and decreased methylation capacity, with neuropathology and aortic lipid deposition. Hum Mol Genet 2001; 10: 433-443 
  29. Perma AF, Ingrosso D, Zappia V, Galletti P, Capasso G, De Santo NG. Enzymatic methyl esterification of erythrocyte membrane proteins is impaired in chronic renal failure: evidence for high levels of the natural inhibitor Sadenosylhomocysteine. J Clin Invest 1993; 91: 2497-2503 
  30. Ueland PM, Hustad S, Schneede J, Refsum H, Vollest Se. Biological and clinical implications of the MTHFR C677T polymorphism. Trends Pharmacol Sci 2002; 22: 195-201 
  31. Park HS, Kim YJ, Ha EH, Lee HY, Chang NS, Hong YC, Pang MG, Kim WK. The risk of MTHFR variants, folate and vitamin $B_{12}$ deficiencies and hyperhomocysteinaemia during pregnancy associated with short gestational age and reduced birth weight. Environ Mutagen Carcinige 2003; 23: 1-6 (Korean) 
  32. Hiltunen MO, Yla-Herttuala S. DNA methylation, smooth muscle cells, and atherogenesis. Arterioscler Thromb Vasc Biol 2003; 23:1750-1753 
  33. Chen JS, Faller DV, Spanjaard RA. Short-chain fatty acid inhibitors of histone deacetylases: promising anticancer therapeutics? Curr Cancer Drug Targets 2003; 3: 219-236 

이 논문을 인용한 문헌 (1)

  1. 2011. "" Nutrition research and practice, 5(2): 112~116 

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