$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

DNA fingerprinting of Brucella abortus isolated from bovine brucellosis outbreaks by repetitive element sequence (rep)-PCR

Abstract

DNA fingerprint patterns of 8 Brucella reference strains and 15 B. abortus field isolates were characterized by repetitive element sequence-based PCR (rep-PCR) using BOX- and ERIC-primers in this study. AMOS PCR differentiated all Brucella field isolates from B. abortus RB51, a vaccine strain by producing a B. abortus-specific 498 bp band. Rep-PCR using BOX-primer produced 13 to 18 bands with sizes of between 230 and 3,300 bp, and discriminated Brucella strains to the species level except B. canis and B. suis. PCR products amplified with ERIC primers were, however, not appropriate for differentiating the Brucella isolates. DNA fingerprint patterns for all B. abortus field isolates were identical among them and were put on one cluster with B. abortus biovar 1 reference strain in the dendrogram, indicating they were highly clonal. These results suggested that rep-PCR using BOX primer might to be a useful tool for calculating genetic relatedness among the Brucella species and for the study of brucellosis epidemiology.

참고문헌 (28)

  1. 국립수의과학검역원. 국립수의과학검역원 연보. pp. 79-90, 국립수의과학검역원, 안양 2003 
  2. Bricker BJ, Ewalt AP, MacMillan AP, Foster G, Brew S. Molecular characterization of Brucella strains isolated from marine mammals. J Clin Microbiol 2000, 38, 1258-1262 
  3. Jersek B, Gilot P, Gubina M, Klun N, Mehle J, Tcherneva E, Rijpens N, Herman L. Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR. J Clin Microbiol 1999, 37, 103-109 
  4. Michaux-Charachon S, Bourg G, Jumas-Bilak E, Guigue-Talat P, Allardet-Servent A, O'Callaghan D, Ramuz M. Genome structure and phylogeny in the genus Brucella. J Bacteriol 1997, 179, 3244-3249 
  5. O'Hara MJ, Collins DM, de Lisle GW. Restriction endonuclease analysis of Brucella ovis and other Brucella species. Vet Microbiol 1985, 10, 425-429 
  6. Versalovic J, Koeuth T, Lupski JR. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucelic Acids Res 1991, 19, 6823-6831 
  7. Vizcaino N, Verger JM, Grayon M, Zygmunt MS, Cloeckaert A. DNA polymorphism at the Omp-31 locus of Brucella spp. Evidence for a large deletion in B. abortus, and other species-specific markers. Microbiology 1997, 143, 2913-2921 
  8. Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990, 18, 7213-7218 
  9. Ewalt DR, Bricker BJ. Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51. J Clin Microbial 2000, 38, 3085-3086 
  10. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994, 32, 2660-2666 
  11. Ficht TA, Husseinen HS, Derr J, Bearden SW. Species-specific sequences at the omp2 locus of Brucella type strains. Int J Syst Bacteriol 1996, 46, 329-331 
  12. Weigel RM, Qiao B, Teferedegne B, Suh DK, Barber DA, Isaacson RE, White BA. Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella. Vet Microbiol 2004, 100, 205-17 
  13. Allardet-Servent A, Bourg G, Ramuz M, Pages M, Bellis M, Roizes G. DNA polymorphism in strains of the genus Brucella. J Bacteriol 1988, 170, 4603-4607 
  14. Chung JS, Cho YJ, Park CK. Isolation and biotyping of Brucella abortus from dairy cattle in Kyungbuk area, Korea. Korean J Vet Res 1988, 28, 339-343 
  15. Verger JM, Grimont F, Grimont PAD, Grayon M. Brucella, monospecific genus as shown by deoxyribonucleic acid hybridization. Int J Syst Bacteriol 1985, 35, 292-295 
  16. Williams E. Brucellosis in humans-its diagnosis and treatment. APMIS Suppl 1988, 3, 21-25 
  17. Tcherneva E, Rijpens N, Naydensky C, Herman L. Repetitive element sequence based on polymerase chain reaction for typing of Brucella strains. Vet Microbiol 1996, 51, 169-178 
  18. Olsen JE, Skov MN, Threlfall EJ, Brown DJ. Clonal lines of Salmonella enterica serotype Enteritidis documented by IS200-, ribo-, and pulsed field gel electrophoresis and RFLP typing. J Med Micribiol 1994, 40, 15-22 
  19. Alton GG, Jones LM, Pietz DE. Laboratory Techniques in Brucellosis. Monograph Series. World Health Organization, Geneva, Switzerland. 1975 
  20. Mercier E, Jumas-Bilak E, Allardet-Servent A, O'Callaghan D, Ramuz M. Polymorphism in Brucella strains detected by studying distribution of two short repetitive DNA elements. J Clin Microbiol 1996, 34, 1299-1302 
  21. Dombek PE, Johnson LK, Zimmerley ST. Use of repetitive DNA sequences and the PCR to discriminate Escherichia coli isolates from human and animal sources. Appl Environ Microbiol 2000, 66, 2572-2577 
  22. Rijpens NJ, Jannes G, Van Asbroeck M, Rossau R, Herman LM. Direct detection of Brucella spp. In raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. Appl Environ Microbiol 1996, 62, 1683-1688 
  23. Burr MD, Josephson KL, Pepper IL. An evaluation of ERIC PCR and AP PCR fingerprinting for discriminating Salmonella serotypes. Lett Appl Microbiol 1998, 27, 24-30 
  24. Tcherneva E, Rijpens N, Jersek B, Herman LMF. Differentiation of Brucella species by random amplified polymorphic DNA analysis. J Appl Microbiol 2000, 88, 69-80 
  25. Fekete A, Bantle JA, Halling SM, Stich RW. Amplification fragment length polymorphism in Brucella strains by use of PCR with arbitrary primers. J Bacteriol 1992, 174, 7778-7783 
  26. Corbel MJ. Brucellosis: an overview. Emerg Infect Dis 1997, 3, 213-221 
  27. Thong KL, Ngeow YF, Altwegg M, Navaratnam P, Pang T. Molecular analysis of Salmonella enteritidis by pulsed-field gel electrophoresis and ribotyping. J Clin Microbiol 1995, 33, 1070-1074 
  28. Jensen AE, Cheville NF, Thoen CO, MacMillan AP, Miller WG. Genomic fingerprinting and development of a dendrogram for Brucella spp. Isolated from seals, porpoises, and dolphins. J Vet Diagn Invest 1999, 11, 152-157 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일