Abstract

Canine coronavirus (CCV) causes a mild gastroenteritis in dogs. The virus is highly contagious. Although the virus was isolated more than thirty years ago, canine coronavirus infection continues to be a widespread problem. Mixed infections with both CCV and canine parvovirus (CPV) are common. Four kinds of commercial killed CCV vaccines are available in Korea. All the commercial vaccines should pass the National Assay for Veterinary Biologicals prior to release. For the potency test of CCV vaccine, it is necessary to use CCV antibody free dogs. The test requires not only kennels but high cost. To develop easy, efficient and economic potency test method for killed CCV vaccine using laboratory animals, a series of experiments with rabbits and guinea pigs were carried out in this study. In the preliminary test, the guinea pigs showed better immune responses than rabbits. The guinea pig was also easy to manage. So guinea pig was selected for the potency test animals. When the guinea pigs were inoculated twice with one dose of vaccine intramuscuarly each, slower and a little lower SN antibody titers were induced in guinea pigs than in dogs (about 2 kg body weight Beagle strain) given the same posology as guinea pigs'. It was concluded that guinea pigs could be substituted for dogs in the potency test of killed CCV vaccine.

주제어

#canine coronavirus   #inactivated vaccine  

참고문헌 (29)

1. 김병한. 최근 3년간 동물용 생물학적제제 수급 동향. 수의과학검역정보 2004, 24, 67-76 
2. 이병형, 전무형, 박종현, 황의경, 허원. 설사증 이환견으로부터 분리한 canine coronavirus의 성상에 관한 연구. 대한수의학회지 1994, 34, 517-527 
3. Appel M, Meunier P, Pollock R, Greisen H, Carmichael L, Glickman L. Canine viral enteritis. A report to practitioners. Canine Pract 1980, 7, 22-36 
4. Bandai C, Ishiguro S, Masuya N, Hohdatsu T, Mochizuki M. Canine coronavirus infections in Japan: Virological and epidemiological aspects. J Vet Med Sci 1999, 61, 731-736 
5. Binn LN, Marchwicki RH, Stephenson EH. Establishment of a canine cell line: Derivation, characterization, and viral spectrum. Am J Vet Res 1980, 41, 855-860 
6. Carmichael LE, Joubert JC, Pollack RVH. Hemagglutination by canine parvovirus: Serological studies and diagnostic applications. Am J Vet Res 1980, 41, 784-791 
7. Cartwright S, Lucas M. Vomiting and diarrhea in dogs. Vet Rec 1972, 91, 571-572 
8. Macartney L, McCandlish IAP, Thompson H, Cornwell HJC. Canine parvovirus enteritis 1: clinical, haematological and pathological features of experimental infection. Vet Rec 1984, 115, 201-210 
9. Tennant BJ, Gaskell RM, Kelly DF, Carter SD. Canine parvovirus infection in the dog following oronasal inoculation. Res Vet Sci 1991, 51, 11-18 
10. Yesilbag K, Yilmaz Z, Torun S, Pratelli A. Canine coronavirus infection in Turkish dog population. J Vet Med 2004, B51, 353-355 
11. Appel MJG. Does canine coronavirus augment the effects of subsequent parvovirus infection? Vet Med 1988, 83, 360-366 
12. Appel MJG, Cooper BJ, Greisen H, Scott F, Carmichael LE. Canine viral enteritis. I. Status report on corona- and parvo-like enteritis. Cornell Vet 1979, 69, 123-133 
13. Tizard IR. Veterinary immunology an introduction. 6th ed. pp. 235-252, Saunders, Philadelphia, 2000 
14. Evermann JF, Foreyt W, Maag-Miller L, Leathers CW, McKeirnan AJ, LeaMaster B. Acute hemorrhagic enteritis associated with canine coronavirus and parvovirus infections in a captive coyote population. J Am Vet Med Assoc 1980, 177, 784-786 
15. Keenan KP, Jervis HR, Marchwicki RH, Binn LN. Intestinal infection of neonatal dogs with canine coronavirus 1-71: studies by virologic, histologic, histochemical, and immunofluorescent techniques. Am J Vet Res 1976, 37, 247-256 
16. 이병형, 전무형, 허원. 국내 사육견에 대한 canine coronavirus 항체 분포 조사. 한국수의공중보건학회지 1993, 17, 295-300 
17. Siddell S, Wege H, Meulen V. The biology of coronaviruses. J Gen Virol 1983, 64, 761-776 
18. Macartney L, McCandlish IAP, Thompson H, Cornwell HJC. Canine parvovirus enteritis 2: pathogenesis. Vet Rec 1984, 115, 453-460 
19. Mills RM. Letters. Aus Vet J 2003, 81, 531 
20. Binn LN, Lazar EC, Keenan KP, Huxsoll DL, Marchwichi RH, Strano AJ. Recovery and characterization of a coronavirus from military dogs with diarrhea. In: Proceedings of the 78th Meeting of the U.S. Animal Health Association. PP. 359-366. Ranoke, 1974 
21. Council of Europe. European Pharmacopoeia. 4th ed. pp. 2245-2284, Council of Europe, Strasbourg, 2001 
22. Carmichael LE. Vaccines for dogs. In: Pastorel PP, Blancou J, Vannier P, Verschueren C (eds.). Veterinary Vaccinology. 1st ed. pp. 326-335, Elservier, New York, 1997 
23. Wesseling JG, Vennema H, Godeke G, Horzinek MC, Rottier PJM. Nucleotide sequence and expression of the spike (S) gene of canine coronavirus and comparison with the S proteins of feline and porcine coronaviruses. J Gen Virol 1994, 75, 1789-1794 
24. 윤기복, 강문일, 박남용, 한동운. 간접형광항체법에 의한 개의 바이러스 - canine distemper virus, canine parvovirus, canine coronavirus, canine adenovirus type-2, canine parainfluenza virus - 항체분포 조사. 대한수의학회지 1995, 35, 75-85 
25. Yasoshima A, Fujinami F, Doi K, Kojima A, Takada H, Okaniwa A. Case report on mixed infection of canine parvovirus and canine coronavirus - Electron microscopy and recovery of canine coronavirus. Jpn J Vet Sci 1983, 45, 217-225 
26. Appel MJG, Cooper BJ, Greisen H, Carmichael LE. Status report: canine viral enteritis. Am J Vet Res 1978, 173, 1516-1518 
27. 사단법인 한국동물약품협회. 동물용의약품등편람. pp. 1888-1898, 경성문화사, 2001 
28. Appel MJ. Virus infections of carnivores. 1st ed. pp. 115-122, Elsevier Science Publishers, Amsterdam, 1987 
29. Appel MJG, Scott FW, Carmichael LE. Isolation and immunization of a canine parvo-like virus from dogs with haemorrhagic enteritis. Vet Rec 1979, 105, 156-159 

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