Abstract

We present a high-throughput continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process. The continuous cell separation chip uses three planar electrodes in a separation channel, where the positive DEP cells are moved away from the central streamline while the negative DEP cells remain in the central streamline. In the experimental study, we use the mixture of viable (live) and nonviable (dead) yeast cells in order to obtain the continuous cell separation conditions. For the conditions of the electric fields frequency of 5MHz and the medium conductivity of $5{\mu}S/cm$, the fabricated chip performs a continuous separation of the yeast cell mixture at the varying flow-rate in the range of $0.1{\sim}{\mu{\ell}/min$.; thereby, resulting in the purity ranges of $95.9{\sim}97.3\%\;and\;64.5{\sim}74.3\%$ respectively for the viable and nonviable yeast cells. present chip demonstrates the constant cell separation performance for varying mixture flow-rates.

주제어

#유전영동   #유체동역학적과정   #연속세포분리  

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참고문헌 (6)

1. Yang, J., Huang, Y., Wang, X-B., Becker, F.F. and Gascoyne, P.R., 1999, 'Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/ Gravitational Field-Flow Fractionation,' Analytical Chemistry, Vol. 71, No.5, pp. 911-918 
2. Markx, G.H. and Pethig, R., 1995, 'Dielectrophoretic Separation of Cells: Continuous Separation,' Biotechnology and Bioengineering, Vol. 45, No.4, pp. 227-343 
3. Huang, Y., Holzel, R., Pethig, R. and Wang, X-B., 1992, 'Difference in the AC Electrodynamics of Viable and Non-viable Yeast Cells Determined Though Combined Dielectrophoresis and Electrotation Studies,' Physics in Medison & Biology, Vol. 37, No.7, pp. 1499-1517 
4. Hughes, M.P., 2002, 'Strategies for Dielectrophoretic Separation in Laboratory-on-a-chip systems,' Electrophoresis, Vol. 23, No. 16, pp. 2569-2582 
5. Yang, J., Huang, Y., Wang, X.-B., Becker, F.F. and Gascoyne, P.R., 1999, 'Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/ Gravitational Field-Flow Fractionation,' Analytical Chemistry, Vol. 71, No.5, pp. 911-918 
6. Stoicheva, N.G., Davey, C.L., Markx, G.H. and Kell, D.B., 1989, 'Dielectric Spectroscopy: A Rapid Method for the Detennination of Solvent Biocompatibility during Biotransformations,' Biocatalysis, Vol. 2, pp. 245-255 

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