• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Intracellular Trafficking of Transferrin-Conjugated Liposome/DNA Complexes by Confocal Microscopy


Intracellular trafficking of transferrin-conjugated dimethyldioctadecyl-ammonium bromide liposome $(T_f-liposome)/DNA$ complexes in HeLa cells was studied using the double-labeled fluorescence technique and confocal microscopy. The size of the $T_f-liposome/DNA$ complex was about 367 nm in diameter and the zeta-potential of it at a 5:1 (w/w) ratio was almost neutral. The intracellular pathway of the $T_f-liposome/DNA$ complex, noted as green (FITC), red (rhodamine) or yellow (FITC + rhodamine) fluorescence, was elucidated from the plasma membrane to the endosome (or lysosome), and finally to the nucleus. The results of this study indicate that plasmid DNA enters into the nucleus not only as a free form but as an associated form complexed with $T_f-liposome$. More interestingly, the $T_f-liposome$ undergoes a nuclear location in the form of ordered structures. This could be a very useful piece of information in designing a safe and advanced gene delivery system.

저자의 다른 논문

참고문헌 (12)

  1. Godbey, W., Wu, T., Kenneth, K., and Mikos, A., Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc. Natl. Acad. Sci. U.S.A., 96, 5177-5181 (1999) 
  2. Mahato, R. I., Rolland, A., and Tomlinson, E., Cationic lipid-based gene delivery systems: pharmaceutical perspectives. Pharm. Res., 14(7), 853-859 (1997) 
  3. Ogris, M., Walker, G.., Blessing, T., Kircheis, R., Wolschek, M., and Wagner, E., Tumor-targeted gene therapy: strategies for the preparation of ligand-polyethylene glycol-polyethylenimine/ DNA complexes. J. Control. Rel., 28, 173-181 (2003) 
  4. Simoes, S., Pires, P., da Cruz M. T., Duzgunes N., and de Lima, M. C., Gene delivery by cationic liposome-DNA complexes containing transferrin or serum albumin. Methods in Enzymology, 373, 369-383 (2003) 
  5. Szoka, F. and Papahadjopoulos, D., Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc. Natl. Acad. Sci. U.S.A., 75, 4194-4198 (1978) 
  6. Wagner, E., Curiel, D., and Cotton, M., Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-mediated endocytosis. Adv. Drug Del. Rev., 14, 113- 135 (1994) 
  7. Carlsson, J., Devin, H., and Axon, R., Protein thiolation and reversible protein-protein conjugation. Biochem. J., 173, 723- 737 (1978) 
  8. Martin, F. J., Health, T. D., and New, R. R. C., Covalent attachment of proteins to liposomes, Liposomes: A practical approach. IRL Press, Oxford, pp. 163-182 (1990) 
  9. Martin, F. J. and Papahadjopoulos, D., Irreversible coupling of immunoglobulin fragments to preformed vesicles: An improved method for liposome targeting. J. Biol. Chem., 257(1), 286- 288 (1982) 
  10. Cheng, P., Receptor ligand-facilitated gene transfer: enhancement of liposome-mediated gene transfer and expression by transferrin. Human Gene Ther., 7(3), 275-282 (1996) 
  11. Thorstensen, K. and Romslo, I., The transferrin receptor: its diagnostic value and its potential as therapeutic target. Scand. J. Clin. Lab Invest. Suppl., 215, 113-120 (1993) 
  12. Gao, X. and Huang, L., Cationic liposome-mediated gene transfer. Gene Ther., 2, 710-722 (1995) 

이 논문을 인용한 문헌 (1)

  1. 2005. "" Macromolecular research, 13(3): 218~222 


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일