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Effects of Mancozeb on the Activities of Murine Peritoneal Macrophages In Vitro and Ex Vivo

Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, v.28 no.1, 2005년, pp.100 - 105  

Chung Ae-Hee (Seoul Metropolitan Government Research Institute of Public and Environment) ,  Pyo Myoung-Yun (College of Pharmacy, Sookmyung Women's University)

Abstract AI-Helper 아이콘AI-Helper

Mancozeb (MCZ) is known to have detrimental effects on the reproductive system, but the toxicity of MCZ on immune responses has not been systematically investigated. We investigated the effects of MCZ exposure on the activities of murine peritoneal macrophages through evaluation of MCZ-induced alter...

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제안 방법

  • In conclusion, on the basis of our data, we propose that MCZ induces an modulation of macrophage activity, in that NO production and TNF-a release are suppressed in vitro and enhanced ex vivo. In order to clearly understand the contrasting effect of MCZ on peritoneal macrophage activity, it is necessary to determine other immunological parameters and to further investigate the influence of m히or metabolite of MCZ (ETU) exposure on the NO production and TNF-a synthesis.
  • With regard to immunotoxicological effects of MCZ, few systemic investigations have been undertaken on human or experimental animal immune system. To initiate delineation of the immunotoxic mechanisms of MCZ, we evaluated the effects of MCZ exposure on activities of macrophages through evaluation of MCZ-induced alteration of NO production, TNF-a synthesis by peritoneal exudates macrophages. Macrophages have been known to play an important role in the host protection and also present antigen to lymphocytes during the development of specific immunity.

대상 데이터

  • Control mice were administered with the same volume of DDW. Five mice were used for each group per experiment and the experiment was performed three times. For evahjation of in vitro MCZ effects, peritoneal macrophages from naive mice untreated with MCZ were exposed to DMEM with 1% DMSO containing 0.
  • Mice were acclimated for 3-4 wks prior to use in our experiments. They were housed in the College of Pharmacy, Sookmyung Women's University (SeouI, Korea) and maintained at 21-24 ℃, 40-60% humi제ty, and 12 h cycle of light/dark. Animals were freely accessible to feed and water.

데이터처리

  • Data were expressed as arithmetical mean ±S.D. Student's f-test was used to establish the differences between control and experimental groups. Significance was concluded at p<0.
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참고문헌 (38)

  1. Binder, C., Schulz, M., Hiddemann, W., and Oellerich, M., Induction of inducible nitric oxide synthase is an essential part of tumor necrosis factor- ${\alpha}$ -induced apoptosis in MCF-7 and other epithelial tumor cells. Lab Invest., 79, 1703-1712 (1999) 

  2. Bisson, M. and Hontela, A., Cytotoxic and endocrine-disrupting potential of atrazine, diazinon, endosulfan, and mancozeb in adrenocortical steroidogenic cells of rainbow Trout exposed in vitro. Toxicol. Appl. Pharmacol., 180, 110-117 (2002) 

  3. Chung, H-T., Pae, H-C., Choi, B-M., Billiar, T. R., and Kim, Y-M., Nitric oxide as a bioregulator of apoptosis. Biochem. Biophys. Res. Commun., 282, 1075-1079 (2001) 

  4. Colosio, C., Barcellini, W., Maroni, M., Alcini, D., Bersani, M., Cavallo, D., Galli, A., Meroni, P., Pastorelli, R., Rozzardi, G. P., Soleo, L., and Foa V., Immunomodulatory effects of occupational exposure to mancozeb. Arch. Environ. Health, 51, 445-451 (1996) 

  5. Colosio, C., Fustinoni, S., Birindelli, S., Bonomi, I., Paschale, G. D., Mammone, T., Tiramani, M., Vercelli, F., Visentin, S., and Maroni, M., Ethylenethiourea in urine as an indicator of exposure to mancozeb in vineyard workers. Toxicol. Lett., 134, 133-140 (2002) 

  6. Daniel, V., Huber, W., Baue, K., Suesal, C., Conradt, C., and Opelz, G., Associations of blood levels of PCB, HCHS, and HCB with numbers of lymphocytes subpopulations, in vitro lymphocyte reponse, plasma cytokine levels, and immunoglobulin autoantibodies. Environ. Health Perspect., 109, 173-178 (2001) 

  7. Estlander, T., Jolanki, R., Henriks-Eckerman, M., and Kanerva, L., Occupational contact allergy to bisphenol A. Contact Dermititis, 40, 52-53 (1999) 

  8. Fine, J. S., Gasiewicz, T. A., and Silverstone, A. E., Lymphocyte stem cell alteration following perinatal exposure to 2,3,7,8- tetrachlorodibenzo-p-dioxin. Mol. Pharmacol., 35, 18-25 (1988) 

  9. Gablicks, J., Askari, E. M., and Yolen, N., DDT and immunological responses. 1. Serum antibodies and anaphylactic shock in guinea-pigs. Arch. Environ. Health, 26, 305 (1973) 

  10. Green, L. C., Wagner, D. A., Glogowski, J. S., Skipper, P. L., Wishnok, J. S., and Tannenbaum, S. R., Analysis of nitrate, nitrite and [15N] nitrite in biological fluids. Analyt. Biochem., 126, 131-138 (1992) 

  11. Gulshan, S., Mccrrden, A. B., and Stimson, W. H., Oestrogen receptors in macrophages. Scand. J. Immunol., 31, 691-697 (1990) 

  12. Hakoda, S., Ishikura, H., Takeyama, N., and Tanaka, T., Tumor necrosis factor- ${\alpha}$ -plus actinomycin D-induced apoptosis of L929 cells is prevented by nitric oxide. Surg Today, 29, 1059- 1067 (1999) 

  13. Kackar, R., Srivastava, M. K., and Raizada, R. B., Studies on rat thyroid after oral administration of mancozeb: Morphological and biochemical evaluations. J. Appl. Toxicol., 17, 369-375 (1997) 

  14. Kiekens, R. C. M., Thepen, T., Ossting, A. J., Bihari, I. C., van de Winkel, J. G. J., Bruijnzeel-Koomen, C. A. F. M., and Knol, E. F., Heterogeneity within tissue-specific macrophage and dendritic cell populations during cutaneous inflammation in atopic dermatitis. Br. J. Dermatol., 145, 957-965 (2001) 

  15. Kim, Y. M., Talanian, R. V., Li, J., and Billiar, T. R., Nitric oxide inhibits apoptosis by preventing increases in caspase-3-like activity via two distinct mechanisms. J. Bio. Chem., 272, 31138-31148 (1997) 

  16. Koch, P., Occupational allergic contact pdermatitis and airborne contact dermatitis from 5 fungicides in a vineyard worker. Cross-reactions between fungicides of the dithiocarbamate group. Contact Dermatitis, 34, 324-329 (1996) 

  17. Kolb, H. and Kolb-Bachofen, V., Nitric oxide in autoimmune disease: Cytotoxic or regulatory mediator. Immunol., Today, 19, 556-561 (1998) 

  18. Lukic, M. L., Popeskovic, L., and Jan, B. D., Potentiation of immune responsiveness in rats treated with DDT. Fed. Proceed., 32, 1037 (1973) 

  19. MacMicking, J., Xie, Q., and Nathan, C., Nitric oxide and macrophage function. Annu. Rev. Immunol., 15, 323-350 (1997) 

  20. Matejuk, A., Adlard, K., Zamora, A., Silverman, M., Vandenbark, A. A., and Offner, H., 17 ${\beta}$ -estradiol inhibits cytokine, chemokine, and chemokine receptor mRNA expression in the central nerveous system of female mice with experimental autoimmune encephalomyelitis. J. Neurosci., 65, 529-542 (2001) 

  21. Mishell, B. B. and Shiigi, S. M., Selected methods in cellular immunology. W. H. Freeman and Company, p.14, (1980) 

  22. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63 (1983) 

  23. Narthan, C. F., Secretory products of macrophages. J. Clin. Invest., 79, 319-326 (1987) 

  24. Ruh, M. F., Bi, Y., Cox, D., Berk, D., Howlett, A. C., and Bellone, C. J., Effect of environmental estrogens on IL-1beta promoter activity in a macrophage cell line. Endocrine, 9, 207-211 (1998) 

  25. Salem, M. L., Matsuzaki, G., Madkour, G. A., and Nomoto, K., Beta-estradiol-induced decrease in IL-12 and TNF- ${\alpha}$ expression suppresses macrophage function in the course of Listeria monocytogenes function in mice. Int. J.Immunopharmacol., 21, 481-497 (1999) 

  26. Shepherd, D. M., Dearstyne, E. A., and Kerkvliet, N. I., The effects of TCDD on the activation of ovalbumin(OVA)-specific DO11.10 transgenic $CD4^+$ T cells in adoptively transferred mice. Toxicol. Science, 56, 340-359 (2000) 

  27. Siddiqui, A., Ali, B., and Srivastava, S. P., Effect of mancozeb on hepatic glutathione S-transferase in rat. Toxicology Letters., 68, 301-305 (1993) 

  28. Stefano, G. B., Prevot, V., Beauvillain, J. C., Fimiani, C., Welters, I., Cadet, P., Breton, C., Pestel, J., Salzet, M., and Bilfinger, T. V., Estradiol coupling to human monocytes nitric oxide release is dependent on intracellular calcium transients: Evidence for an estrogen surface receptor. J. Immunol., 163, 3758-5763 (1999) 

  29. Thomas, P. S., Tumor necrosis factor- ${\alpha}$ : The role of this multifunctional cytokine in asthma. Immunol. Cell. Biol., 79, 132-140 (2001) 

  30. Underhill, D. M. and Ozinsky, A., Phagocytosis of microbes: Complexity in action. Annu. Rev. Immunol., 20, 825-852 (2002) 

  31. USA Environmental Protection Agency (EPA), Mancozeb Pesticide Fact Sheet (1995) 

  32. Van Dervort, A. L., Yan, L., Madara, P. J., Cobb, J. P., Wesley, R. A., Corriveau, C. C., et al., Nitric oxide regulates endotoxin induced TNF- ${\alpha}$ production by human neutrophils. J. Immunol., 152, 4102-4109 (1994) 

  33. Vegeto, E., Bonincontro, C., Pollio, G., Sala, A., Viappiani, S., Nardi, F., Brusadelli, A., Viviani, B., Ciana, P., and Maggi, A., Estrogen prevents the lipopolysaccharide-induced inflammatory response in microglia. J. Neurosci., 21, 1809- 1818 (2001) 

  34. Vergova, M., Jablonica, A., and Janota, S., Occupational exposure to mancozeb in employees in the production of Novozir Mn 80. Prakov Lak, 40, 425-430 (1988) 

  35. Vermeulen, L. A., Reinecke, A. J., and Reinecke, S. A., Evaluation of the fungicide manganese-zinc ethylene bis(dithiocarbamate) (mancozeb) for sublethal and acute toxicity to Eisenia fetida(Oligochaeta). Ecotoxico. Environ. Saf., 48, 183-189 (2001) 

  36. Woodfork, K. A., Schuller, K. C., and Huffman, L. J., Cytokine and nitric oxide release by J774A.1 macrophages is not regulated by estradiol. Life Sci., 69, 2287-2294 (2001) 

  37. Xing, Z., Current understanding of macrophage type 1 cytokine responses during intracellular infections. Histol. Histopathol., 15, 199-205 (2000) 

  38. Zhang, X., Wang, L., Zhang, H., Guo, D., Qiao, Z., and Qiao, J., Estrogen inhibits lipopolysaccharide-induced tumor necrosis factor-alpha release from murine macrophages. Methods Fund. Exp. Clin. Pharmacol., 23, 169-173 (2001) 

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