This study was conducted to examine the physiological and biochemical responses against UV-B radiation in the seedling of 15 different rice cultivars, having the different physiological sensitivities. Out of 15 rice cultivars tested, moderate and susceptible groups showed significant decreases in bi...
This study was conducted to examine the physiological and biochemical responses against UV-B radiation in the seedling of 15 different rice cultivars, having the different physiological sensitivities. Out of 15 rice cultivars tested, moderate and susceptible groups showed significant decreases in biomass and RGR (relative growth rate). Contents of total chlorophyll were reduced remarkedly by irradiation of UV-B. In all rice cultivars tested, the content of chlorophyll a was strongly decreased, while the contents of chlorophyll b were slightly reduced without showing clear different among three groups and 15 cultivars. Carotenoid content was largely reduced by UV-B radiation, whereas polyamine content was moderately increased. The contents of MDA (malondialdehyde) that reflect the level of lipid peroxidation of cell membranes were clearly increased by UV-B stress, showing higher content in susceptible cultivars than moderate and torelant cultivars. The physiological important parameters highly related to visible injury were leaf color, chlorophyll, carotenoid, and lipid peroxidation, whereas biomass and polyamines were not closely correlated. Based on this results, it was concluded that changes of visible injury and the contents of chlorophyll and MDA could be adequately applied and utilized as physiological indicators to UV-B radiation.
This study was conducted to examine the physiological and biochemical responses against UV-B radiation in the seedling of 15 different rice cultivars, having the different physiological sensitivities. Out of 15 rice cultivars tested, moderate and susceptible groups showed significant decreases in biomass and RGR (relative growth rate). Contents of total chlorophyll were reduced remarkedly by irradiation of UV-B. In all rice cultivars tested, the content of chlorophyll a was strongly decreased, while the contents of chlorophyll b were slightly reduced without showing clear different among three groups and 15 cultivars. Carotenoid content was largely reduced by UV-B radiation, whereas polyamine content was moderately increased. The contents of MDA (malondialdehyde) that reflect the level of lipid peroxidation of cell membranes were clearly increased by UV-B stress, showing higher content in susceptible cultivars than moderate and torelant cultivars. The physiological important parameters highly related to visible injury were leaf color, chlorophyll, carotenoid, and lipid peroxidation, whereas biomass and polyamines were not closely correlated. Based on this results, it was concluded that changes of visible injury and the contents of chlorophyll and MDA could be adequately applied and utilized as physiological indicators to UV-B radiation.
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문제 정의
, 1989) are discussed. This study was conducted to determine and evaluate the intraspecific differences in physiological and biochemical responses of 15 rice cultivars to UV-B radiation in a growth chamber under the absence or presence ofsupplemental levels of UVB radiation.
가설 설정
Fresh weights and growth rates ofrice seedling m three different groups of 15 cultivars, tolerant, moderate, and susceptible, were shown on Table 1. The irradiation of UV-B to the rice seedlings for a short time did remarkably influence m the reduction offresh weight. The reduction rate offresh weight of three different groups, tolerant, moderate, and susceptible, were 21.
Considerung the examined results of fresh weight, Odaebyeo, Jinbong- byeo, Woonjangbyeo, Seojinbyeo, and Gancheokbyeo were resistant cultivars to UV-B radiation. The contents of chloro phyll a and b and total chlorophyll in the leaves of nee seed ling treated with the irradiation of UV-B were shown on Table 2. The contents of chlorophyll a were remarkedly reduced with the treatment of UV-B radiation. The reduction rates of tolerate, moderate, susceptible cultivars were about 29.
성능/효과
6% respectively. Among the three groups of fifteen cultivars which were selected and grouped based on the damage level on nee seedling leaves by the irradiation of UV-B, the effect of UV-B on fresh weight have appeared little different, although it was reduced the highest m UV-B moderate nee seedlings and reduced higher in UV-B susceptible than those in UV-B tolerant rice seed lings. The relative growth rates (RGR) of rice seedlings with the treatment of UV-B radiation were ranged from 0.
05). Considering the examined results of chlorophyll a and b, Odaebyeo, Daeyabyeo, and Woonjangbyeo were resistant cultivars to UV-B radiation.
17 (mg g-1 day-1). Considerung the examined results of fresh weight, Odaebyeo, Jinbong- byeo, Woonjangbyeo, Seojinbyeo, and Gancheokbyeo were resistant cultivars to UV-B radiation. The contents of chloro phyll a and b and total chlorophyll in the leaves of nee seed ling treated with the irradiation of UV-B were shown on Table 2.
The lipid peroxidation was distinctly induced by UV-B stress. The MDA contents in three rice cultivar groups, tolerant, moderate, and susceptible, were increased by 27.7%, 115.4%, and 373.7%, respectively. Particularly, the content was increased remarkedly in the sus ceptible rice cultivars.
The irradiation of UV-B to the rice seedlings for a short time did remarkably influence m the reduction offresh weight. The reduction rate offresh weight of three different groups, tolerant, moderate, and susceptible, were 21.2%, 46.9% and 33.6% respectively. Among the three groups of fifteen cultivars which were selected and grouped based on the damage level on nee seedling leaves by the irradiation of UV-B, the effect of UV-B on fresh weight have appeared little different, although it was reduced the highest m UV-B moderate nee seedlings and reduced higher in UV-B susceptible than those in UV-B tolerant rice seed lings.
The contents of chlorophyll a were remarkedly reduced with the treatment of UV-B radiation. The reduction rates of tolerate, moderate, susceptible cultivars were about 29.1%, 37.2%, and 53.7%, respectively. The contents of chlorophyll b were not clearly different among three groups of cultivars.
It would be assumed that the damages of rice seedling leaves by UV-B radiation have closely related to the reduction of carotenoid content. Unlike the content of carotenoid, the content of polyamines of three groups, toler ant, moderate, and susceptible, were increased by 89.4%, 19.9%, and 13.9%, respectively. The lipid peroxidation was distinctly induced by UV-B stress.
후속연구
, 1994). The UV-B tolerant cultivars identified in this study might be used as materials for breeding programs; however, the genetic basis for these differences must be further examined. In a soybean study, the effect of UV-B on total plant biomass may be dependent on the concentration of UV-B absorbing compounds (D'sumey et al.
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