$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental factors are critical players in cellular damage and aging. In order to develop a new antiphotoaging agent, this work focused on the antioxidant effects of the extract of tinged autumnal leaves of Acer palmatum. One compound was isolated from an ethyl acetate soluble fraction of the A. palmatum extract using silica gel column chromatography. The chemical structure was identified as apigenin-8-C-beta-D-glucopyranoside, more commonly known as vitexin, by spectral analysis including LC-MS, FT-IR, UV, $^{1}H-$, and $^{13}C-NMR$. The biological activities of vitexin were investigated for the potential application of its anti-aging effects in the cosmetic field. Vitexin inhibited superoxide radicals by about $70\%$ at a concentration of $100\;{\mu}g/mL$ and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals by about $60\%$ at a concentration of $100\;{\mu}g/mL$. Intracellular ROS scavenging activity was indicated by increases in dichlorofluorescein (DCF) fluorescence upon exposure to UVB $20\;mJ/cm^2$ in cultured human dermal fibroblasts (HDFs) after the treatment of vitexin. The results show that oxidation of 5-(6-)chloromethyl-2',7'-dichlo-rodihydrofluorescein diacetate ($CM-H_{2}DCFDA$) is inhibited by vitexin effectively and that vitexin has a potent free radical scavenging activity in UVB-irradiated HDFs. In ROS imaging using a confocal microscope we visualized DCF fluorescence in HDFs directly. In conclusion, our findings suggest that vitexin can be effectively used for the prevention of UV-induced adverse skin reactions such as free radical production and skin cell damage.

참고문헌 (38)

  1. Bestwick, C. S. and Milne, L., Quercetin modifies reactive oxygen levels but exerts only partial protection against oxidative stress within HL-60 cells. Biochim. Biophys. Acta, 1528, 48-59 (2001) 
  2. Blois, M. S., Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200 (1958) 
  3. Bonina, F., Puglia, C., Ventura, D., Aquino, R., Tortora, S., Sacchi, A., Saija, A., Tomaino, A., Pellegrino, M. L., and de Capariis P., In vitro antioxidant and in vivo photoprotective effects of a lyophilized extract of Capparis spinosa L. buds. J. Cosmet. Sci., 53, 321-335 (2002) 
  4. Cathcart, R., Schwiers, E., and Ames, B. N., Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay. Anal. Biochem., 134, 111-116 (1983) 
  5. Harborne, J. B., Nature, Distribution and function of plant flavonoids. Prog. Clin. Biol. Res., 213, 15-24 (1986) 
  6. Harman, D., Role of free radical reactions in aging and disease. J. Geriat. Dermatol., 5, 114-127 (1997) 
  7. Mathews-Roth, M. M., Carotenoid pigment administration and the delay in development of UVB-induced tumors. Photochem. Photobiol., 42, 35-38 (1983) 
  8. Oh, I. S., Whang, W. K., and Kim, I. H., Constituents of Crataegus pinnatifida val. psilosa Leaves (II) Flavonoids from BuOH fraction. Arch. Pharm. Res., 17, 314-317 (1994) 
  9. Prabhakar, M. C., Bano, H., Kumar, I., Shamsi, M. A., and Khan, M. S. Y., Pharmacological investigations on vitexin. Planta medica, 43, 396-403 (1981) 
  10. Trayner, I. D., Rayner, A. P., Freeamn, G. E., and Farzaneh, F., Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate ($H_2DCFDA$) or dihydrorhodamine 123 ($H_2R123$). J. Immunol. Methods, 186, 275-284 (1995) 
  11. Zhang, H., Joseph, J., Felix, C., and Kalyanaraman, B., Bicarbonate enhances the hydroxylation, nitration, and peroxidation reactions catalyzed by copper, zinc superoxide dismutase : intermediacy of carbomate anion radical. J. Biol. Chem., 275, 14038-14045 (2000) 
  12. Afitlhile, M. M., Dent, R. M., and Cowan, A. K., Changes in carotenoid composition in senescing leaves of Hordeum vulgare L. cv. Dyan. J. Plant Physiol., 142, 4349 (1993) 
  13. Middleton, E. Jr., Kandaswami, C., and Theoharides, T. C., The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol. Rev., 52, 673-751 (2000) 
  14. Savini, I., DAngelo, I., Ranalli, M., Melino, G., and Avigliano, L., Ascorbic acid maintenance in HaCaT cells prevents radical formation and apoptosis by UVB. Free Radic. Biol. Med., 26, 1172-1180 (1999) 
  15. Gaitan, E., Cooksey, R. C., Legan, J., and Lindsay, R. H., Antithyroid effects in vivo and in vitro of vitexin : a Cglycosylflavone in millet. J. Clin. Endocrinol. Metab., 80, 1144-1147 (1995) 
  16. Ryoo, Y. W., Suh, S. I., Mun, K. C., Kim, B. C., and Lee, K. S., The effects of the melatonin on ultraviolet-B irradiated cultured dermal fibroblasts. J. Dermatol. Sci., 27, 162-169 (2001) 
  17. Young, A. J., Wellings R., and Britton G., The fate of chloroplast pigments during senescence of primary leaves of Hordeum vulgare and Avena sativum. J. Plant Physiol., 137, 701-705 (1991) 
  18. Furuno, K., Akasako, T., and Sugihara, N., The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids. Biol. Pharm. Bull., 25, 19-23 (2002) 
  19. Bandoniene, D. and Murkovic, M., On-line HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.). J. Agric. Food Chem., 50, 2482-2487 (2002) 
  20. LeBel, C. P., Ischiropoulos, H., and Bondy, S. C., Evaluation of the probe 2,7-dichlorofluorescein as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol., 5, 227-231 (1992) 
  21. Riipi, M., Ossipov V., Lempa, K., Haukioja, E., Koricheva, J., Ossipova, S., and Pihlaja, K., Seasonal changes in birch leaf chemistry: are there trade-offs between leaf growth and accumulation of phenolics? Oecologia, 130, 380-390 (2002) 
  22. Katiyar, S. K., Afaq, F., Perex, A., and Mukhtar, H., Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress. Carcinogenesis, 22, 287-294 (2001) 
  23. Nijveldt, R. J., Nood, E., Hoorn, D. EC, Boelens, P. G., Norren, K., and Leeuwen, P.AM, Flavonoids: a review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr., 74, 418-425 (2001) 
  24. Yoo, S. W., Kim, J. S., Kang, S. S., Son, K. H., Chang, H. W., Kim, H. P., Bae, K. H., and Lee, C. O., Constituents of the fruits and leaves of Euodia danielli. Arch. Pharm. Res., 25, 824-830 (2002) 
  25. Dauborn, B. and Brueggemann, W., A spontaneous point mutation in rubisco large subunit gene impairing holoenzyme assemblyrenders the IV beta plastome mutant of Oenothera extremely light and chilling sensitive. Physiol. Plant, 104, 116-124 (1998) 
  26. Feild, T. S., Lee, D. W., and Holbrook, N. M., Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood, Plant Physiol., 127, 566-574 (2001) 
  27. Norins, A. L., Free radical formation in the skin following exposure to ultraviolet light. J. Invest. Dermatol., 39, 445-448 (1962) 
  28. Aritomi, M., Chemical constituents in Aceraceous plants. 1. Flavonoid constituents in the leaves of Acer palmatum Thunberg. Yakugaku Zasshi, 83, 737-740 (1963) 
  29. Lee, B. C., Bae, J. T., Pyo, H. B., Choe, T. B., Kim, S. W., Hwang, H. J., and Yun, J. W., Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola Frondosa. Enzyme Microb. Technol., 6274, 1-8 (2003) 
  30. Seo, S. Y., Kim, E. Y., Kim, H., and Gwang, B. J., Neuroprotective effect of high glucose agains NMDA, free radical and oxygen-glucose deprivation through enhanced mitochondrial potentials. J. Neurosci., 19, 8849-8855 (1999) 
  31. Greenham, J., Harborne, J. B., and Williams, C. A., Identification of lipophilic flavones and flavonols by comparative HPLC, TLC, and UV spectral analysis. Phytochem. Anal., 14, 100-118 (2003) 
  32. Tampo, Y., Kotamraju, S., Chitambar, C. R., Kalivendi, S. V., Keszler, A., Joseph, J., and Kalyanaraman, B., Oxidative stress-induced iron signaling is responsible for peroxidedependent oxidation of dichlorodihydrofluorescein in endothelial cells. Circ. Res., 92, 56-63 (2003) 
  33. Bors, W. and Michel, C., Chemistry of the antioxidant effect of polyphenols. Ann. N. Y. Acad. Sci., 957, 57-69 (2002) 
  34. Khettab, N., Amory, M. C., Brigand, G., Bousquet, B., and Combre, A., Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis. Biochimie, 70, 1709-1713 (1988) 
  35. Tobi, S. E., Gilbert, M., Paul, N., and McMillan, T. J., The green tea polyphenol, epigallocatechin-3-gallate, protects against the oxidative cellular and genotoxic damage of UVA radiation. Int. J. Cancer, 102, 439-444 (2002) 
  36. Shibamoto, T., Sulfur-containing heterocyclic compounds with antioxidative activity. Am. Chem. Soc., 564, 247-256 (1994) 
  37. Ham, I. H., Oh, I. S., Whang, W. K., and Kim I. H., Pharmacoconstituents of Korean cultivated Rhubarb leaves-the flavonoids from leaves. Yakhak Hoeji, 38, 469-475 (1994) 
  38. Aritomi, M., Chemical constituents in Aceraceous plants. 3. Flavonoid constituents in leaves of Acer cissifolium K. Koch. Chem. Pharm. Bull., 12, 841-843 (1964) 

이 논문을 인용한 문헌 (6)

  1. 2007. "" Journal of applied biological chemistry, 50(4): 215~220 
  2. 2007. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 30(10): 1293~1301 
  3. 2007. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 30(9): 1116~1123 
  4. Kim, Soo-Hwa ; Jung, Hee ; Shin, Yong-Cheol ; Ko, Seong-Gyu 2008. "Research of Traditional Herbal Medicines for Anti-aging, Inhibition Effect of Wrinkle and Whitening Effect in the Skin" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 22(3): 691~698 
  5. 2009. "" 목재공학 = Journal of the Korean wood science and technology, 37(2): 171~176 
  6. Lee, Min-Ki ; Park, Suk-Hoon ; Kim, Sun-Ju 2011. "A time-course study of flavonoids in buckwheats (Fagopyrum species)" 농업과학연구 = CNU Journal of agricultural science, 38(1): 87~94 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일