OLUT Ali I.
(Department of Infectious Diseases, Tepecik SSK Training Hospital)
,
ERGUVEN Sibel
(Department of Parasitology and Hacettepe University Hospital)
,
EMRI Salih
(Department of Chest Diseases, Hacettepe University Hospital)
,
OZUNLU Haluk
(Department of Infectious Diseases, Tepecik SSK Training Hospital)
,
AKAY Hadi
(Department of Chest Surgery, Ankara University Ibni Sina Hospital)
The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In this study the capabilities of a new rapid test, the hydatid antigen d...
The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In this study the capabilities of a new rapid test, the hydatid antigen dot immunobinding assay (HA-DIA), which was developed for the diagnosis of pulmonary hydatidosis, were studied and compared with another immunodiagnostic method, indirect hemagglutination (IHA). The study subjects included 18 patients, 9 women, 9 men; range 7 to 63 years; mean 30 years, with surgically proven pulmonary hydatidosis, a control group comprised of 14 patients; viral respiratory infections (1), cirrhosis (2), connective tissue disease (2), taeniasis (3), and 6 healthy donors. We found that the HA-DIA test had a sensitivity of $67\%$ and specificity of $100\%$, and that the IHA test had a sensitivity of $50\%$ and specificity of $100\%$. We conclude that HA-DIA is a simple, rapid, low cost assay that does not require instrumentation and has a higher sensitivity than IHA for the diagnosis of pulmonary hydatidosis.
The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In this study the capabilities of a new rapid test, the hydatid antigen dot immunobinding assay (HA-DIA), which was developed for the diagnosis of pulmonary hydatidosis, were studied and compared with another immunodiagnostic method, indirect hemagglutination (IHA). The study subjects included 18 patients, 9 women, 9 men; range 7 to 63 years; mean 30 years, with surgically proven pulmonary hydatidosis, a control group comprised of 14 patients; viral respiratory infections (1), cirrhosis (2), connective tissue disease (2), taeniasis (3), and 6 healthy donors. We found that the HA-DIA test had a sensitivity of $67\%$ and specificity of $100\%$, and that the IHA test had a sensitivity of $50\%$ and specificity of $100\%$. We conclude that HA-DIA is a simple, rapid, low cost assay that does not require instrumentation and has a higher sensitivity than IHA for the diagnosis of pulmonary hydatidosis.
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문제 정의
HA-DIA allows detection of the Echinococcus specific antibodies and is a form of dot immunobinding assay (DIA). The aim of this study was to determine the sensitivity and specificity of the HA-DIA assay and to compare its validity ver sus indirect hemagglutination (IHA) testing for the diagnosis of pulmonary hydatidosis.
제안 방법
HA-DIA is a new technology developed for the diagnosis of human hydatidosis that allows the detection of Echinococcus granulosus specific antibodies in blood samples (Echinostrip, Lofarma Laboratories, Milan). The test measures serum antigen binding, which is positively related to the concentration of the antibody specific for hydatid antigen. The antigen used in this test is obtained from hydatid fluid collect ed from fertile cysts in bovine livers and lungs, and bound to nitrocellulose reactive areas on plastic stick supports.
성능/효과
Another interesting finding was that, positive rates of IHA and HA-DIA tests were higher in post-opera tive cases than in pre-operative cases, although this difference was not statistically significant (p > 0.05). This might also have been due to an increased release of antigens to the circulation as a result of surgical manipulation.
(1992), which evalu ated eight serological tests for the diagnosis of human hydatidosis, the most sensitive serological test was found to be IgG ELISA (as has been found by many other studies) with a sensitivity of 94% for liver dis eases and 62% for lung diseases. In that study, the sensitivity of IHA for pulmonary hydatidosis was found to be 56%, which is similar to our finding of 50%. In our study, the sensitivity of HA-DIA was 67%, which appeared a little more sensitive than the IgG ELISA results of Force et al.
참고문헌 (5)
Babba H, Messedi A, Masmoudi S (1994) Diagnosis of human hydatidosis: Comparison between imagery and six serologic techniques. Am J Trap Med Hyg 50: 64-68
Devi CS, Parija SC (2003) A new serum hydatid antigen detection test for diagnosis of cystic echinococcosis. Am J Trap Med Hyg 69: 525-528
Force L, Torres JM, Carillo A, Busca J (1992) Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up. Clin Infect Dis 15: 473-480
King CH (2000) Echinococcosis. In Principles and Practice of Infectious Diseases, Mandell GL, Bennet JE, Dolin R (eds.). p2962-2963, Churchill Livingstone 5th ed. New York, USA
Misterello G, Gentili M, Falagini P, Roncarolo D, Riva G, Tinelli M (1995) Dot immunobinding assay as a new diagnostic test for human hydatid disease. Immunol Lett 47: 79-85
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