$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"

과학기술 지식인프라 ScienceON

상세검색 다국어 입력
도움말도움말
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

MyON담기
내보내기

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection

The Korean journal of parasitology v.43 no.1 = no.133 , 2005년, pp.19 - 25  
CHO Jung-Hwa (Department of Biology, College of Natural Science, Chung-Ang University, Department of Tropical and Endemic Parasitic Diseases, National Institute of Health ) ;  CHUNG Woo-Suk ( National Veterinary Research and Quarantine Service ) ;  SONG Kyoung-Ju ( Department of Microbiology, Ajou University School of Medicine ) ;  NA Byoung-Kuk ( Department of Molecular Parasitology, Sungkyunkwan University School of Medicine ) ;  KANG Seung-Won ( National Veterinary Research and Quarantine Service ) ;  SONG Chul-Yong ( Department of Biology, College of Natural Science, Chung-Ang University ) ;  KIM Tong-Soo ( Department of Tropical and Endemic Parasitic Diseases, National Institute of Health)
Abstract

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N. caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo.

주제어

#Neospora caninum   #recombinant antigens   #vaccine  

저자의 다른 논문

송철용(23)

참고문헌 (14)

  1. Andrianarivo AG, Choromanski L, McDonough SP, Packham AE, Conrad PA (1999) Immunogenicity of a killed Neospora caninum tachyzoite preparation formulated with different adjuvants. Int J Parasitol 29: 1613-1625 
  2. Augustine PC, Jenkins MC, Dubey JP (1999) Effect of polyclonal antisera developed against dense granule-associated Neospora caninum proteins on cell invasion and development in vitro by N. caninum tachyzoites. Parasitology 119: 441-445 
  3. Cannas A, Naguleswaran A, Muller N, Eperon S, Gottstein B, Hemphill A (2003) Vaccination of mice against experimental Neospora caninum infection using NcSAG1- and NcSRS2-based recombinant antigens and DNA vaccines. Parasitology 126: 303-312 
  4. Long MT, Baszler TV, Mathison BA (1998) Comparison of intracerebral parasite load, lesion development, and systemic cytokines in mouse strains infected with Neospora caninum. J Parasitol 84: 316-320 
  5. Romero JJ, Perez E, Frankena K (2004) Effect of a killed whole Neospora caninum tachyzoite vaccine on the crude abortion rate of Costa Rican dairy cows under field conditions. Vet Parasitol 123: 149-159 
  6. Innes EA, Andrianarivo AG, Bjorkman C, Williams DJ, Conrad PA (2002) Immune responses to Neospora caninum and prospects for vaccination. Trends Parasitol 18: 497-504 
  7. Choromanski L, Block W (2000) Humoral immune responses and safety of experimental formulations of inactivated Neospora vaccines. Parasitol Res 86: 851-853 
  8. Dubey JP, Lindsay DS (1996) A review of Neospora caninum and neosporosis. Vet Parasitol 67: 1-59 
  9. Howe DK, Crawford AC, Lindsay D, Sibley LD (1998) The p29 and p35 immunodominant antigens of Neospora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii. Infect Immun 66: 5322-532 
  10. Liddell S, Jenkins MC, Collica CM, Dubey JP (1999) Prevention of vertical transfer of Neospora caninum in BALB/c mice by vaccination. J Parasitol 85: 1072-1075 
  11. Mutapi F, Roddam A (2002) P values for pathogens: statistical inference from infectious-disease data. Lancet Infect Dis 2: 219-230 
  12. Dubey JP (2003) Review of Neospora caninum and neosporosis in animals. Korean J Parasitol 41: 1-16 
  13. Nishikawa Y, Xuan X, Nagasawa H, Igarashi I, Fujisaki K, Otsuka H, Mikami T (2001) Prevention of vertical transmission of Neospora caninum in BALB/c mice by recombinant vaccinia virus carrying NcSRS2 gene. Vaccine 19: 1710-1716 
  14. Hemphill A, Gottstein B (1996) Identification of a major surface protein on Neospora caninum tachyzoites. Parasitol Res 82: 497-504 

이 논문을 인용한 문헌 (1)

  1. Lee, Sang-Eun ; Lee, Jung-Youn 2008. "Detection of Neospora caninum in the blood of Korean native cattle and dairy cows using PCR" 大韓獸醫學會誌 = Korean journal of veterinary research, 48(2): 191~195 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

이 논문과 연관된 기능

DOI 인용 스타일