[논문]Optimum Conditions for the Biological Production of Lactic Acid by a Newly Isolated Lactic Acid Bacterium, Lactobacillus sp. RKY2

Wee Young-Jung; Kim Jin-Nam; Yun Jong-Sun; Ryu Hwa-Won

doi:10.1007/bf02931178

Optimum Conditions for the Biological Production of Lactic Acid by a Newly Isolated Lactic Acid Bacterium, Lactobacillus sp. RKY2 원문보기

Biotechnology and bioprocess engineering : Bbe, v.10 no.1, 2005년, pp.23 - 28

Wee Young-Jung (School of Biological Sciences and Technology, Chonnam National University) , Kim Jin-Nam (Department of Material Chemical and Biochemical Engineering, Chonnam National University) , Yun Jong-Sun (BioHelix, Biotechnology Industrialization Center) , Ryu Hwa-Won (School of Biological Sciences and Technology, Chonnam National University)

Abstract ▼ AI-Helper

Lactic acid is a green chemical that can be used as a raw material for biodegradable polymer. To produce lactic acid through microbial fermentation, we previously screened a novel lactic acid bacterium. In this work, we optimized lactic acid fermentation using a newly isolated and homofermentative lactic acid bacterium. The optimum medium components were found to be glucose, yeast extract, $(NH_4)_{2}HPO_4,\;and\;MnSO_4$. The optimum pH and temperature for a batch culture of Lactobacillus sp. RKY2 was found to be 6.0 and $36^{\circ}C$, respectively. Under the optimized culture conditions, the maximum lactic acid concentration (153.9 g/L) was obtained from 200 g/L of glucose and 15 g/L of yeast extract, and maximum lactic acid productivity ($6.21\;gL^{-1}h^{-1}$) was obtained from 100 g/L of glucose and 20 g/L of yeast extract. In all cases, the lactic acid yields were found to be above 0.91 g/g. This article provides the optimized conditions for a batch culture of Lactobacillus sp. RKY2, which resulted in highest productivity of lactic acid.

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대상 데이터

Lactobacillus sp. RKY2 [17, 18], which was screened from soybean paste, was used for all experiments. This species was previously deposited to the Korean Collection for Type Cultures (Daejon? Korea) as KCTC 10353BP.

이론/모형

6 mL/min, while the column temperature was maintained at 35°C. Glucose was measured enzymatically by a glu cose oxidase peroxidase method using a kit from YD Di agnostics (Seoul, Korea). All the experiments were car ried out in duplicate and the mean values are reported.

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