[논문]Selection of Mediators for Bioelectrochemical Nitrate Reduction

Kim Seung Hwan; Song Seung Hoon; Yoo Young Je

doi:10.1007/bf02931182

Selection of Mediators for Bioelectrochemical Nitrate Reduction 원문보기

Biotechnology and bioprocess engineering : Bbe, v.10 no.1, 2005년, pp.47 - 51

Kim Seung Hwan (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University) , Song Seung Hoon (Bio-MAX Institute, Seoul National University) , Yoo Young Je (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, School of Chemical Engineering, Seoul National University)

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The bioelectrochemical reduction of nitrate in the presence of various mediators including methyl viologen and azure A was studied using a 3-electrode voltammetric system. The catalytic potential for the reduction of the mediators was observed in the reactor, which for methyl viologen and azure A were -0.74 V and -0.32 V, respectively, with respect to the potential of Ag/AgCl reference electrode. This potential was then applied to a working electrode to reduce each mediator for enzymatic nitrate reduction. Nitrite, the product of the reaction, was measured to observe the enzymatic nitrate reduction in the reaction media. Methyl viologen was observed as the most efficient mediator among those tested, while azure A showed the highest electron efficiency at the intrinsic reduction potential when the mediated enzyme reactions were carried out with the freely solubilized mediator. The electron transfer of azure A with respect to time was due to the adhesion of azure A to the hydrophilic surface during the reduction. In addition, the use of the adsorbed mediator on conductive activated carbon was proposed to inhibit the change in the electron transfer rate during the reaction by maintaining a constant mediator concentration and active surface area of the electrode. Azure A showed better than nitrite formation than methyl viologen when used with activated carbon.

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1. A commercial Ag/AgCI electrode was used as the reference electrode and graphite (diameter, 5 mm; length, 15 mm), from the Sigma-Aldrich Company (USA), as the material for the working and counter electrodes. Both the current and potential were measured at the working, reference and counter electrodes.

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