Kim Kyoung-Cheol
(Department of Civil, Geosystem and Environmental Engineering, College of Engineering, Chonnam National University)
,
Kim Si-Wouk
(Department of Environmental Engineering, Chosun University)
,
Kim Myong-Jun
(Department of Civil, Geosystem and Environmental Engineering, College of Engineering, Chonnam National University)
,
Kim Seong-Jun
(Department of Civil, Geosystem and Environmental Engineering, College of Engineering, Chonnam National University)
The study was targeted to saccharify foodwastes with the cellulolytic and amylolytic enzymes obtained from culture supernatant of Trichoderma harzianum FJ1 and analyze the kinetics of the saccharification in order to enlarge the utilization in industrial application. T. harzianum FJ1 highly produced...
The study was targeted to saccharify foodwastes with the cellulolytic and amylolytic enzymes obtained from culture supernatant of Trichoderma harzianum FJ1 and analyze the kinetics of the saccharification in order to enlarge the utilization in industrial application. T. harzianum FJ1 highly produced various cellulolytic (filter paperase 0.9, carboxymethyl cellulase 22.0, ${\beta}$-glucosidase 1.2, Avicelase 0.4, xylanase 30.8, as U/mL-supernatant) and amylolytic (${alpha}$-amylase 5.6, ${\beta}$-amylase 3.1, glucoamylase 2.6, as U/mL-supernatant) enzymes. The $23{\sim}98\;g/L$ of reducing sugars were obtained under various experimental conditions by changing FPase to between $0.2{\sim}0.6\;U/mL$ and foodwastes between $5{\sim}20\%$ (w/v), with fixed conditions at $50^{\circ}C$, pH 5.0, and 100 rpm for 24 h. As the enzymatic hydrolysis of foodwastes were performed in a heterogeneous solid-liquid reaction system, it was significantly influenced by enzyme and substrate concentrations used, where the pH and temperature were fixed at their experimental optima of 5.0 and $50^{\circ}C$, respectively. An empirical model was employed to simplify the kinetics of the saccharification reaction. The reducing sugars concentration (X, g/L) in the saccharification reaction was expressed by a power curve ($X=K{\cdot}t^n$) for the reaction time (t), where the coefficient, K and n. were related to functions of the enzymes concentrations (E) and foodwastes concentrations (S), as follow: $K=10.894{\cdot}Ln(E{\cdot}S^2)-56.768,\;n=0.0608{\cdot}(E/S)^{-0.2130}$. The kinetic developed to analyze the effective saccharification of foodwastes composed of complex organic compounds could adequately explain the cases under various saccharification conditions. The kinetics results would be available for reducing sugars production processes, with the reducing sugars obtained at a lower cost can be used as carbon and energy sources in various fermentation industries.
The study was targeted to saccharify foodwastes with the cellulolytic and amylolytic enzymes obtained from culture supernatant of Trichoderma harzianum FJ1 and analyze the kinetics of the saccharification in order to enlarge the utilization in industrial application. T. harzianum FJ1 highly produced various cellulolytic (filter paperase 0.9, carboxymethyl cellulase 22.0, ${\beta}$-glucosidase 1.2, Avicelase 0.4, xylanase 30.8, as U/mL-supernatant) and amylolytic (${alpha}$-amylase 5.6, ${\beta}$-amylase 3.1, glucoamylase 2.6, as U/mL-supernatant) enzymes. The $23{\sim}98\;g/L$ of reducing sugars were obtained under various experimental conditions by changing FPase to between $0.2{\sim}0.6\;U/mL$ and foodwastes between $5{\sim}20\%$ (w/v), with fixed conditions at $50^{\circ}C$, pH 5.0, and 100 rpm for 24 h. As the enzymatic hydrolysis of foodwastes were performed in a heterogeneous solid-liquid reaction system, it was significantly influenced by enzyme and substrate concentrations used, where the pH and temperature were fixed at their experimental optima of 5.0 and $50^{\circ}C$, respectively. An empirical model was employed to simplify the kinetics of the saccharification reaction. The reducing sugars concentration (X, g/L) in the saccharification reaction was expressed by a power curve ($X=K{\cdot}t^n$) for the reaction time (t), where the coefficient, K and n. were related to functions of the enzymes concentrations (E) and foodwastes concentrations (S), as follow: $K=10.894{\cdot}Ln(E{\cdot}S^2)-56.768,\;n=0.0608{\cdot}(E/S)^{-0.2130}$. The kinetic developed to analyze the effective saccharification of foodwastes composed of complex organic compounds could adequately explain the cases under various saccharification conditions. The kinetics results would be available for reducing sugars production processes, with the reducing sugars obtained at a lower cost can be used as carbon and energy sources in various fermentation industries.
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제안 방법
In this study, to analyze the kinetics of the enzymatic hydrolysis of foodwastes, the temperature and pH were fixed at the optimum values throughout the experiments, with the enzyme and foodwastes concentrations in the kinetic model considered as important factors. The kinetic model, X = K-tn, where X is conversion ratio (%), t is reaction time (hr), and K and n are empirical constants was introduced.
The amylolytic enzymes, composed of a-amylase, P-amylase and glucoamylase, can be combined, and substituted with a-amylase for soluble starch hydrolysis activity. Therefore, a-amylase and FPase activities were monitored as the combined cellulolytic and amylolytic activities, respectively, in this study.
This study focused on development of an economical and efficient recovery method or producing reducing sugars from Korean foodwastes for use as carbon and energy sources in various fermentation industries, lb improve the yield and rate of enzymatic hydrolysis of foodwastes, effective saccharification factors, such as substrate concentration, enzyme concentration, reaction time, temperature and pH, will be experimentally optimized. For the kinetics of the enzymatic hydrolysis reaction, the empirical models concerning two variables, the enzyme and foodwastes concentration, will be developed and applied in these kinetics analysis.
This study focused on the recovery of useful resources, simultaneously satisfying the treatment of foodwastes. In the composition of foodwastes, polymers with a high reducing ability, that is, with large energy content, such as amylose, cellulose and hemicellulose were converted to glucose and xylose by enzymatic hydrolysis involving various lignocellulolytic enzymes.
대상 데이터
ATP and Rest were collected from the storage tanks of apartments and restaurants around Gwangju city, respectively.
이론/모형
In this study, the reducing sugars concentration to the reaction time in the enzymatic hydrolysis of foodwastes composed of complex materials was expressed as a function of the power curve equation, which has been used by Wu and Ju [3이 and Park et al. [2이 etc as an empirical model.
0 citric acid buffer and a pertinent amount of each substrate, with the reaction run in a shaking water bath at 50℃, 100 rpm for 48 h. The reducing sugars concentration of the reaction supernatant was measured by the DNS method. The saccharification ratio of pure cellulosic materials was calculated using Eq.
참고문헌 (32)
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