Yoo Eun-Jeong
(Department of Biotechnology, Yosu National University)
,
Lim Hyun-Soo
(Department of Biotechnology, Yosu National University)
,
Park Kyung-Ok
(Department of Biotechnology, Yosu National University)
,
Choi Myeong-Rak
(Department of Biotechnology, Yosu National University)
This study was performed to know whether there is any change of physiological activity in DLMJ which is inoculated by lactic acid bacteria. Lactic acid bacteria were isolated from Dolsan leaf mustard Kimchi (DLMK) at $20^{\circ}C$. In the optimum ripening period, the population of Leucono...
This study was performed to know whether there is any change of physiological activity in DLMJ which is inoculated by lactic acid bacteria. Lactic acid bacteria were isolated from Dolsan leaf mustard Kimchi (DLMK) at $20^{\circ}C$. In the optimum ripening period, the population of Leuconostoc and Lactobacilli in the DLMK were found to be high. The Leuconostoc, Lactobacilli and Lactococci strains were identified as Leuconostoc mesenteroides, Leuconostoc gelidum, Weissella confusa, Lactobacillus plantarum, Lactobacillus raffinolactis, Lactococcus lactis and Weissella confusa using the Biolog system. The most predominant strain which was isolated from DLMK was Weissella confusa. As the results of the phylogenetic analysis using 16s rDNA sequence, the Weissella confusa turned out to be Weissella kimchii, with 99.0% similarity. To investigated the change of physiological activity in DLMJ by lactic acid bacteria, 7 predominant strains inoculated to DLMJ (Dolsan Leaf Mustard Juice). The cytotoxicity was found to be under $19.55\%$ all cases. Also, the antioxidative activity of the DLMJ treated with lactic acid bacteria was very low, which might have been due to the reduced antioxidative phytochemicals during the preparation of the sterile sample. The ACE inhibiting activity of DLMJ by inoculation with Weissella kimchii was shown to be the highest ($94.0\%$). This could be that the degradation of sulfur containing materials in DLMJ by Weissella kimchii gave rise to ACE inhibiting activity.
This study was performed to know whether there is any change of physiological activity in DLMJ which is inoculated by lactic acid bacteria. Lactic acid bacteria were isolated from Dolsan leaf mustard Kimchi (DLMK) at $20^{\circ}C$. In the optimum ripening period, the population of Leuconostoc and Lactobacilli in the DLMK were found to be high. The Leuconostoc, Lactobacilli and Lactococci strains were identified as Leuconostoc mesenteroides, Leuconostoc gelidum, Weissella confusa, Lactobacillus plantarum, Lactobacillus raffinolactis, Lactococcus lactis and Weissella confusa using the Biolog system. The most predominant strain which was isolated from DLMK was Weissella confusa. As the results of the phylogenetic analysis using 16s rDNA sequence, the Weissella confusa turned out to be Weissella kimchii, with 99.0% similarity. To investigated the change of physiological activity in DLMJ by lactic acid bacteria, 7 predominant strains inoculated to DLMJ (Dolsan Leaf Mustard Juice). The cytotoxicity was found to be under $19.55\%$ all cases. Also, the antioxidative activity of the DLMJ treated with lactic acid bacteria was very low, which might have been due to the reduced antioxidative phytochemicals during the preparation of the sterile sample. The ACE inhibiting activity of DLMJ by inoculation with Weissella kimchii was shown to be the highest ($94.0\%$). This could be that the degradation of sulfur containing materials in DLMJ by Weissella kimchii gave rise to ACE inhibiting activity.
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가설 설정
So, needs to study for the role of lactic acid bacteria to the change of physiological activities in DLMJ are existed. Accordingly, this study was performed to know whether there is any change of physiological activity in DLMJ which is inoculated by lactic acid bacteria. Thus, physio logical activities were examined for cytotoxicity, antioxi dative, and ACE inhibiting activities.
제안 방법
In a previous paper [21], the physiological activity in DLMK juice during the fermentation period increased significantly with an increase in the growth of microbes. In order to investigate the effect of the microbes on the physiological activity in DLMJ, the microbes in DLMK we isolated and identified using selective media. Fig.
대상 데이터
The leaf mustard was obtained from Dolsan, Jeol- lanamdo, Korea, cut into 2 to 3 cm sizes, washed twice with distilled water and then crushed (Hanil, HMF-340, Seoul, Korea) and filtered through sterilized gauze. The filtrate was centrifuged at 1, 500 rpm for 15 min, the su pernatant filtered through a Whatman No.
이론/모형
The antioxidative activity in DLMJ due to the addition of various lactic acid bacteria was determined using the DPPH (af-diphenyl--picrylhydrazyl) method [2기. 5 mL of the DPPH was added to 1 mL of sample and left to stand for 30 min.
성능/효과
Consequently, these results suggested that ACE activity inhibitory compounds are closely related to the metabo lite production of extracellular enzymes by the Weissella kimchii Dolsan in DLMJ. However, the antihypertensive active compounds of Weissella kimchii Dolsan in leaf mustard remain unknown.
The cytotoxicities were higher than those of the control (DLM|, itself), with the exception of Lactococcus lacis Dolsan inoculation. However, the cytotoxicities toward DLMJ by the addition of Leuconostoc mesenteroides Dolsan, Lactobacillus delbrueckii lactis Dolsan and Weis- sella kim사lii Dolsan were all under 20.0%. DLM con tains thiosulfates and organosulfur compounds, which are known to inhibit chemically-induced tumors [31].
As 7 is이ated predominant strains were added to DLMJ, the ACE inhibiting activity increased more than that toward DLMJ. The ACE inhibiting activity toward DLMJ by the addition of Leuconostoc mesenteroides Dolsan, Leuconostoc gelidum Dolsan and Weissella kimchii Dolsan were 88.8, 87.7 and 94.0%, respectively. Hence, the DLMJ on the addition of Weissella kimchii Dolsan showed the maxi mum value of 94, 0%.
2 shows the phylogenetic tree of Weissella confusa Dolsan. The Weissella kimchii was the closest phyloge netic relative of Weissella confusa Dolsan, with a 16S rRNA similarity of 99.0%. Nam et al.
후속연구
However, the antihypertensive active compounds of Weissella kimchii Dolsan in leaf mustard remain unknown. Further studies will be needed to clarify the ACE inhibiting activity of DLMJ and iden tify and purify the active compounds in DLMJ.
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